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Bifidobacterium infantis 35624 protects against salmonella-induced reductions in digestive enzyme activity in mice by attenuation of the host inflammatory response.

Symonds EL, O'Mahony C, Lapthorne S, O'Mahony D, Sharry JM, O'Mahony L, Shanahan F - Clin Transl Gastroenterol (2012)

Bottom Line: Typhimurium infection significantly reduced the activity of all brush border enzymes in a dose- and time-dependent manner (P<0.05).Salmonella infection reduces the small intestinal brush border enzyme activity in mice, with the level of reduction and associated weight loss increasing with dose and duration of infection.B. longum subsp. infantis 35624 treatment attenuated the effect of Salmonella infection on brush border enzyme activity and weight loss, which may be due to modulation of the host immune response.

View Article: PubMed Central - PubMed

Affiliation: Alimentary Pharmabiotic Centre, National University Ireland, Cork, Ireland.

ABSTRACT

Objectives: Salmonella-induced damage to the small intestine may decrease the villi-associated enzyme activity, causing malabsorption of nutrients and diarrhea, and thus contribute to the symptoms of infection. The objective of this study was to determine the mechanism by which different doses and durations of Salmonella infection and lipopolysaccharide (LPS) affect brush border enzyme activity in the mouse, and to determine if the probiotic Bifidobacterium longum subspecies infantis 35624 could attenuate the intestinal damage.

Methods: BALB/c mice were challenged with Salmonella enterica serovar Typhimurium UK1 at various doses (10(2)-10(8) colony-forming unit (CFU)) and durations (10(6) CFU for 1-6 days). Mice were also treated with B. longum subsp. infantis 35624 for 2 weeks before and during a 6-day S. Typhimurium challenge (10(6) CFU), or before injection of LPS. The small intestine was assessed for morphological changes, mRNA expression of cytokines, and activity of the brush border enzymes sucrase-isomaltase, maltase, and alkaline phosphatase.

Results: S. Typhimurium infection significantly reduced the activity of all brush border enzymes in a dose- and time-dependent manner (P<0.05). This also occurred following injection of LPS. Pre-treatment with B. longum subsp. infantis 35624 prevented weight loss, protected brush border enzyme activity, reduced the small intestinal damage, and inhibited the increase in interleukin (IL)-10 and IL-8 expression due to Salmonella challenge.

Conclusions: Salmonella infection reduces the small intestinal brush border enzyme activity in mice, with the level of reduction and associated weight loss increasing with dose and duration of infection. B. longum subsp. infantis 35624 treatment attenuated the effect of Salmonella infection on brush border enzyme activity and weight loss, which may be due to modulation of the host immune response.

No MeSH data available.


Related in: MedlinePlus

Levels of viable B. longum subsp. infantis 35624 in the cecum, proximal and distal colon (gray), and in the corresponding luminal contents (striped). Data are mean±s.e., with *indicating P<0.05. N=6 per group. CFU, colony-forming unit.
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fig4: Levels of viable B. longum subsp. infantis 35624 in the cecum, proximal and distal colon (gray), and in the corresponding luminal contents (striped). Data are mean±s.e., with *indicating P<0.05. N=6 per group. CFU, colony-forming unit.

Mentions: Viable B. longum subsp. infantis 35624 was not detected either in the small intestinal tissue or contents, or in the spleen or in the liver. It was detected in the cecal tissue and contents (at similar levels), but was at 102- to 103-fold higher levels in the proximal and distal colon luminal contents compared with their corresponding tissue sections (Figure 4). Consumption of B. longum subsp. infantis 35624 (for 3 weeks) did not significantly alter the brush border enzyme activity (P>0.05) or the villi or crypt length of the jejunum (P>0.05, data not shown).


Bifidobacterium infantis 35624 protects against salmonella-induced reductions in digestive enzyme activity in mice by attenuation of the host inflammatory response.

Symonds EL, O'Mahony C, Lapthorne S, O'Mahony D, Sharry JM, O'Mahony L, Shanahan F - Clin Transl Gastroenterol (2012)

Levels of viable B. longum subsp. infantis 35624 in the cecum, proximal and distal colon (gray), and in the corresponding luminal contents (striped). Data are mean±s.e., with *indicating P<0.05. N=6 per group. CFU, colony-forming unit.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3367613&req=5

fig4: Levels of viable B. longum subsp. infantis 35624 in the cecum, proximal and distal colon (gray), and in the corresponding luminal contents (striped). Data are mean±s.e., with *indicating P<0.05. N=6 per group. CFU, colony-forming unit.
Mentions: Viable B. longum subsp. infantis 35624 was not detected either in the small intestinal tissue or contents, or in the spleen or in the liver. It was detected in the cecal tissue and contents (at similar levels), but was at 102- to 103-fold higher levels in the proximal and distal colon luminal contents compared with their corresponding tissue sections (Figure 4). Consumption of B. longum subsp. infantis 35624 (for 3 weeks) did not significantly alter the brush border enzyme activity (P>0.05) or the villi or crypt length of the jejunum (P>0.05, data not shown).

Bottom Line: Typhimurium infection significantly reduced the activity of all brush border enzymes in a dose- and time-dependent manner (P<0.05).Salmonella infection reduces the small intestinal brush border enzyme activity in mice, with the level of reduction and associated weight loss increasing with dose and duration of infection.B. longum subsp. infantis 35624 treatment attenuated the effect of Salmonella infection on brush border enzyme activity and weight loss, which may be due to modulation of the host immune response.

View Article: PubMed Central - PubMed

Affiliation: Alimentary Pharmabiotic Centre, National University Ireland, Cork, Ireland.

ABSTRACT

Objectives: Salmonella-induced damage to the small intestine may decrease the villi-associated enzyme activity, causing malabsorption of nutrients and diarrhea, and thus contribute to the symptoms of infection. The objective of this study was to determine the mechanism by which different doses and durations of Salmonella infection and lipopolysaccharide (LPS) affect brush border enzyme activity in the mouse, and to determine if the probiotic Bifidobacterium longum subspecies infantis 35624 could attenuate the intestinal damage.

Methods: BALB/c mice were challenged with Salmonella enterica serovar Typhimurium UK1 at various doses (10(2)-10(8) colony-forming unit (CFU)) and durations (10(6) CFU for 1-6 days). Mice were also treated with B. longum subsp. infantis 35624 for 2 weeks before and during a 6-day S. Typhimurium challenge (10(6) CFU), or before injection of LPS. The small intestine was assessed for morphological changes, mRNA expression of cytokines, and activity of the brush border enzymes sucrase-isomaltase, maltase, and alkaline phosphatase.

Results: S. Typhimurium infection significantly reduced the activity of all brush border enzymes in a dose- and time-dependent manner (P<0.05). This also occurred following injection of LPS. Pre-treatment with B. longum subsp. infantis 35624 prevented weight loss, protected brush border enzyme activity, reduced the small intestinal damage, and inhibited the increase in interleukin (IL)-10 and IL-8 expression due to Salmonella challenge.

Conclusions: Salmonella infection reduces the small intestinal brush border enzyme activity in mice, with the level of reduction and associated weight loss increasing with dose and duration of infection. B. longum subsp. infantis 35624 treatment attenuated the effect of Salmonella infection on brush border enzyme activity and weight loss, which may be due to modulation of the host immune response.

No MeSH data available.


Related in: MedlinePlus