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Bifidobacterium infantis 35624 protects against salmonella-induced reductions in digestive enzyme activity in mice by attenuation of the host inflammatory response.

Symonds EL, O'Mahony C, Lapthorne S, O'Mahony D, Sharry JM, O'Mahony L, Shanahan F - Clin Transl Gastroenterol (2012)

Bottom Line: Typhimurium infection significantly reduced the activity of all brush border enzymes in a dose- and time-dependent manner (P<0.05).Salmonella infection reduces the small intestinal brush border enzyme activity in mice, with the level of reduction and associated weight loss increasing with dose and duration of infection.B. longum subsp. infantis 35624 treatment attenuated the effect of Salmonella infection on brush border enzyme activity and weight loss, which may be due to modulation of the host immune response.

View Article: PubMed Central - PubMed

Affiliation: Alimentary Pharmabiotic Centre, National University Ireland, Cork, Ireland.

ABSTRACT

Objectives: Salmonella-induced damage to the small intestine may decrease the villi-associated enzyme activity, causing malabsorption of nutrients and diarrhea, and thus contribute to the symptoms of infection. The objective of this study was to determine the mechanism by which different doses and durations of Salmonella infection and lipopolysaccharide (LPS) affect brush border enzyme activity in the mouse, and to determine if the probiotic Bifidobacterium longum subspecies infantis 35624 could attenuate the intestinal damage.

Methods: BALB/c mice were challenged with Salmonella enterica serovar Typhimurium UK1 at various doses (10(2)-10(8) colony-forming unit (CFU)) and durations (10(6) CFU for 1-6 days). Mice were also treated with B. longum subsp. infantis 35624 for 2 weeks before and during a 6-day S. Typhimurium challenge (10(6) CFU), or before injection of LPS. The small intestine was assessed for morphological changes, mRNA expression of cytokines, and activity of the brush border enzymes sucrase-isomaltase, maltase, and alkaline phosphatase.

Results: S. Typhimurium infection significantly reduced the activity of all brush border enzymes in a dose- and time-dependent manner (P<0.05). This also occurred following injection of LPS. Pre-treatment with B. longum subsp. infantis 35624 prevented weight loss, protected brush border enzyme activity, reduced the small intestinal damage, and inhibited the increase in interleukin (IL)-10 and IL-8 expression due to Salmonella challenge.

Conclusions: Salmonella infection reduces the small intestinal brush border enzyme activity in mice, with the level of reduction and associated weight loss increasing with dose and duration of infection. B. longum subsp. infantis 35624 treatment attenuated the effect of Salmonella infection on brush border enzyme activity and weight loss, which may be due to modulation of the host immune response.

No MeSH data available.


Related in: MedlinePlus

Brush border enzyme activity of (a) the jejunum and (b) the ileum over 6 days of infection with 106S. Typhimurium UK1 (n=10 per group). The results are expressed as % of non-infected value, with diamonds representing sucrase–isomaltase activity, squares representing maltase activity and the broken line representing alkaline phosphatase activity. Data are mean±s.e., with “a” indicative of P<0.05 compared with the sucrase–isomaltase non-infected values, “b” indicative of P<0.05 compared with the maltase non-infected values and “c” indicative of P<0.05 compared with the alkaline phosphatase non-infected values. CFU, colony-forming unit.
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fig2: Brush border enzyme activity of (a) the jejunum and (b) the ileum over 6 days of infection with 106S. Typhimurium UK1 (n=10 per group). The results are expressed as % of non-infected value, with diamonds representing sucrase–isomaltase activity, squares representing maltase activity and the broken line representing alkaline phosphatase activity. Data are mean±s.e., with “a” indicative of P<0.05 compared with the sucrase–isomaltase non-infected values, “b” indicative of P<0.05 compared with the maltase non-infected values and “c” indicative of P<0.05 compared with the alkaline phosphatase non-infected values. CFU, colony-forming unit.

Mentions: Duration of Salmonella infection was associated with weight loss (r=−0.521, P<0.001) and became significant at day 6 compared with non-infected mice (weight change compared with baseline: non-infected mice=1.41±0.87%, day 1=−2.16±0.97%, day 3=−1.32±0.71%, day 6=−6.64±2.34%, P<0.005). Following 24 h of infection, Salmonella was detected in the liver, spleen, and small intestine, and these levels did not significantly change over the 6 days of infection (Table 3; P>0.05). There were significant changes in the enzyme activity, with sucrase–isomaltase, maltase, and ALP activity in the jejunum significantly reduced at all days of infection (P<0.0001; Figure 2a), whereas in the ileum the changes were more variable, with a decrease in maltase activity at all days post-infection, but a decrease in sucrase–isomaltase activity after only 3 days (Figure 2b). There was a negative correlation between the activity of all enzymes and duration of infection in the jejunum (sucrase–isomaltase r=−0.679, maltase r=−0.632, ALP r=−0.510, P<0.005), and for sucrase–isomaltase and maltase in the ileum (sucrase–isomaltase r=−0.748, maltase r=−0.470, P<0.005).


Bifidobacterium infantis 35624 protects against salmonella-induced reductions in digestive enzyme activity in mice by attenuation of the host inflammatory response.

Symonds EL, O'Mahony C, Lapthorne S, O'Mahony D, Sharry JM, O'Mahony L, Shanahan F - Clin Transl Gastroenterol (2012)

Brush border enzyme activity of (a) the jejunum and (b) the ileum over 6 days of infection with 106S. Typhimurium UK1 (n=10 per group). The results are expressed as % of non-infected value, with diamonds representing sucrase–isomaltase activity, squares representing maltase activity and the broken line representing alkaline phosphatase activity. Data are mean±s.e., with “a” indicative of P<0.05 compared with the sucrase–isomaltase non-infected values, “b” indicative of P<0.05 compared with the maltase non-infected values and “c” indicative of P<0.05 compared with the alkaline phosphatase non-infected values. CFU, colony-forming unit.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3367613&req=5

fig2: Brush border enzyme activity of (a) the jejunum and (b) the ileum over 6 days of infection with 106S. Typhimurium UK1 (n=10 per group). The results are expressed as % of non-infected value, with diamonds representing sucrase–isomaltase activity, squares representing maltase activity and the broken line representing alkaline phosphatase activity. Data are mean±s.e., with “a” indicative of P<0.05 compared with the sucrase–isomaltase non-infected values, “b” indicative of P<0.05 compared with the maltase non-infected values and “c” indicative of P<0.05 compared with the alkaline phosphatase non-infected values. CFU, colony-forming unit.
Mentions: Duration of Salmonella infection was associated with weight loss (r=−0.521, P<0.001) and became significant at day 6 compared with non-infected mice (weight change compared with baseline: non-infected mice=1.41±0.87%, day 1=−2.16±0.97%, day 3=−1.32±0.71%, day 6=−6.64±2.34%, P<0.005). Following 24 h of infection, Salmonella was detected in the liver, spleen, and small intestine, and these levels did not significantly change over the 6 days of infection (Table 3; P>0.05). There were significant changes in the enzyme activity, with sucrase–isomaltase, maltase, and ALP activity in the jejunum significantly reduced at all days of infection (P<0.0001; Figure 2a), whereas in the ileum the changes were more variable, with a decrease in maltase activity at all days post-infection, but a decrease in sucrase–isomaltase activity after only 3 days (Figure 2b). There was a negative correlation between the activity of all enzymes and duration of infection in the jejunum (sucrase–isomaltase r=−0.679, maltase r=−0.632, ALP r=−0.510, P<0.005), and for sucrase–isomaltase and maltase in the ileum (sucrase–isomaltase r=−0.748, maltase r=−0.470, P<0.005).

Bottom Line: Typhimurium infection significantly reduced the activity of all brush border enzymes in a dose- and time-dependent manner (P<0.05).Salmonella infection reduces the small intestinal brush border enzyme activity in mice, with the level of reduction and associated weight loss increasing with dose and duration of infection.B. longum subsp. infantis 35624 treatment attenuated the effect of Salmonella infection on brush border enzyme activity and weight loss, which may be due to modulation of the host immune response.

View Article: PubMed Central - PubMed

Affiliation: Alimentary Pharmabiotic Centre, National University Ireland, Cork, Ireland.

ABSTRACT

Objectives: Salmonella-induced damage to the small intestine may decrease the villi-associated enzyme activity, causing malabsorption of nutrients and diarrhea, and thus contribute to the symptoms of infection. The objective of this study was to determine the mechanism by which different doses and durations of Salmonella infection and lipopolysaccharide (LPS) affect brush border enzyme activity in the mouse, and to determine if the probiotic Bifidobacterium longum subspecies infantis 35624 could attenuate the intestinal damage.

Methods: BALB/c mice were challenged with Salmonella enterica serovar Typhimurium UK1 at various doses (10(2)-10(8) colony-forming unit (CFU)) and durations (10(6) CFU for 1-6 days). Mice were also treated with B. longum subsp. infantis 35624 for 2 weeks before and during a 6-day S. Typhimurium challenge (10(6) CFU), or before injection of LPS. The small intestine was assessed for morphological changes, mRNA expression of cytokines, and activity of the brush border enzymes sucrase-isomaltase, maltase, and alkaline phosphatase.

Results: S. Typhimurium infection significantly reduced the activity of all brush border enzymes in a dose- and time-dependent manner (P<0.05). This also occurred following injection of LPS. Pre-treatment with B. longum subsp. infantis 35624 prevented weight loss, protected brush border enzyme activity, reduced the small intestinal damage, and inhibited the increase in interleukin (IL)-10 and IL-8 expression due to Salmonella challenge.

Conclusions: Salmonella infection reduces the small intestinal brush border enzyme activity in mice, with the level of reduction and associated weight loss increasing with dose and duration of infection. B. longum subsp. infantis 35624 treatment attenuated the effect of Salmonella infection on brush border enzyme activity and weight loss, which may be due to modulation of the host immune response.

No MeSH data available.


Related in: MedlinePlus