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Community-acquired methicillin-resistant Staphylococcus aureus, Uruguay.

Ma XX, Galiana A, Pedreira W, Mowszowicz M, Christophersen I, Machiavello S, Lope L, Benaderet S, Buela F, Vincentino W, Albini M, Bertaux O, Constenla I, Bagnulo H, Llosa L, Ito T, Hiramatsu K - Emerging Infect. Dis. (2005)

Bottom Line: A novel, methicillin-resistant [corrected] Staphylococcus aureus clone (Uruguay clone) with a non-multidrug-resistant phenotype caused a large outbreak, including 7 deaths, in Montevideo, Uruguay.The clone was distinct from the highly virulent community clone represented by strain MW2, although both clones carried Panton-Valentine leukocidin gene and cna gene.

View Article: PubMed Central - PubMed

Affiliation: Juntendo University, Tokyo, Japan.

ABSTRACT
A novel, methicillin-resistant [corrected] Staphylococcus aureus clone (Uruguay clone) with a non-multidrug-resistant phenotype caused a large outbreak, including 7 deaths, in Montevideo, Uruguay. The clone was distinct from the highly virulent community clone represented by strain MW2, although both clones carried Panton-Valentine leukocidin gene and cna gene.

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Related in: MedlinePlus

Dendrogram of pulsed-field gel electrophoresis (PFGE) banding pattern ofrepresentative Uruguay clone. Pulsotypes of representative Uruguay strains,a CA-MRSA strain isolated in the United States (MW2), 3 CA-MRSA strainsisolated in Australia (A803355, A823549, and E802537), and a Japanese strainisolated from an outpatient (81/108) were compared by using a BioNumericssoftware program (Applied Maths, Sint-Martens-Latem, Belgium). Similaritycoefficient was calculated by using Pearson correlation with positiontolerance of 5%, and cluster analysis was performed by the unweightedpair-group method. Pulsotypes in parentheses indicate the types previouslyreported (7). PFGE was performed for22 h with a CHEF MAPPER (Bio-Rad, Hercules, CA, USA) with a pulse time of 5s to 40 s. SmaI-restriction patterns of the tested strainsand reference strains were compared by using BioNumerics software. Genotypesof representative strains were determined by multilocus sequence typing asdescribed by Enright et al. (9).Sequence type (ST) and clonal complex were assigned using programs in theS. aureus multilocus sequence typing database(http://www.mlst.net).
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Figure 2: Dendrogram of pulsed-field gel electrophoresis (PFGE) banding pattern ofrepresentative Uruguay clone. Pulsotypes of representative Uruguay strains,a CA-MRSA strain isolated in the United States (MW2), 3 CA-MRSA strainsisolated in Australia (A803355, A823549, and E802537), and a Japanese strainisolated from an outpatient (81/108) were compared by using a BioNumericssoftware program (Applied Maths, Sint-Martens-Latem, Belgium). Similaritycoefficient was calculated by using Pearson correlation with positiontolerance of 5%, and cluster analysis was performed by the unweightedpair-group method. Pulsotypes in parentheses indicate the types previouslyreported (7). PFGE was performed for22 h with a CHEF MAPPER (Bio-Rad, Hercules, CA, USA) with a pulse time of 5s to 40 s. SmaI-restriction patterns of the tested strainsand reference strains were compared by using BioNumerics software. Genotypesof representative strains were determined by multilocus sequence typing asdescribed by Enright et al. (9).Sequence type (ST) and clonal complex were assigned using programs in theS. aureus multilocus sequence typing database(http://www.mlst.net).

Mentions: We compared characteristics of outbreak strains with those of previously investigatedCA-MRSA strains isolated in the United States (MW2) and Australia (A803355, A823549,and E802537), and a strain isolated from an outpatient in Japan in 1981 (81/108)(Figure 2). All of them possessed bothcna andlukS,F-PV genes as well. Thedominant outbreak strains belonged to ST-30, which was the same as Australian andJapanese strains and distinct from MW2 (ST-1). In addition, we found that thepulsotype of strain A803355 (reported previously as H1) was identical to pulsotypeA1, the most representative pulsotype among tested strains. Pulsotypes of other 2Australian strains, A823549 and E8025347, were classified into the same cluster asA, while that of 81/108 showed a similar type on pulsed-field gel electrophoresis(PFGE) to pulsotype A. In contrast, only 2 Uruguay isolates,UR20 and UR41, had aPFGE pattern similar to that of MW2.


Community-acquired methicillin-resistant Staphylococcus aureus, Uruguay.

Ma XX, Galiana A, Pedreira W, Mowszowicz M, Christophersen I, Machiavello S, Lope L, Benaderet S, Buela F, Vincentino W, Albini M, Bertaux O, Constenla I, Bagnulo H, Llosa L, Ito T, Hiramatsu K - Emerging Infect. Dis. (2005)

Dendrogram of pulsed-field gel electrophoresis (PFGE) banding pattern ofrepresentative Uruguay clone. Pulsotypes of representative Uruguay strains,a CA-MRSA strain isolated in the United States (MW2), 3 CA-MRSA strainsisolated in Australia (A803355, A823549, and E802537), and a Japanese strainisolated from an outpatient (81/108) were compared by using a BioNumericssoftware program (Applied Maths, Sint-Martens-Latem, Belgium). Similaritycoefficient was calculated by using Pearson correlation with positiontolerance of 5%, and cluster analysis was performed by the unweightedpair-group method. Pulsotypes in parentheses indicate the types previouslyreported (7). PFGE was performed for22 h with a CHEF MAPPER (Bio-Rad, Hercules, CA, USA) with a pulse time of 5s to 40 s. SmaI-restriction patterns of the tested strainsand reference strains were compared by using BioNumerics software. Genotypesof representative strains were determined by multilocus sequence typing asdescribed by Enright et al. (9).Sequence type (ST) and clonal complex were assigned using programs in theS. aureus multilocus sequence typing database(http://www.mlst.net).
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3367603&req=5

Figure 2: Dendrogram of pulsed-field gel electrophoresis (PFGE) banding pattern ofrepresentative Uruguay clone. Pulsotypes of representative Uruguay strains,a CA-MRSA strain isolated in the United States (MW2), 3 CA-MRSA strainsisolated in Australia (A803355, A823549, and E802537), and a Japanese strainisolated from an outpatient (81/108) were compared by using a BioNumericssoftware program (Applied Maths, Sint-Martens-Latem, Belgium). Similaritycoefficient was calculated by using Pearson correlation with positiontolerance of 5%, and cluster analysis was performed by the unweightedpair-group method. Pulsotypes in parentheses indicate the types previouslyreported (7). PFGE was performed for22 h with a CHEF MAPPER (Bio-Rad, Hercules, CA, USA) with a pulse time of 5s to 40 s. SmaI-restriction patterns of the tested strainsand reference strains were compared by using BioNumerics software. Genotypesof representative strains were determined by multilocus sequence typing asdescribed by Enright et al. (9).Sequence type (ST) and clonal complex were assigned using programs in theS. aureus multilocus sequence typing database(http://www.mlst.net).
Mentions: We compared characteristics of outbreak strains with those of previously investigatedCA-MRSA strains isolated in the United States (MW2) and Australia (A803355, A823549,and E802537), and a strain isolated from an outpatient in Japan in 1981 (81/108)(Figure 2). All of them possessed bothcna andlukS,F-PV genes as well. Thedominant outbreak strains belonged to ST-30, which was the same as Australian andJapanese strains and distinct from MW2 (ST-1). In addition, we found that thepulsotype of strain A803355 (reported previously as H1) was identical to pulsotypeA1, the most representative pulsotype among tested strains. Pulsotypes of other 2Australian strains, A823549 and E8025347, were classified into the same cluster asA, while that of 81/108 showed a similar type on pulsed-field gel electrophoresis(PFGE) to pulsotype A. In contrast, only 2 Uruguay isolates,UR20 and UR41, had aPFGE pattern similar to that of MW2.

Bottom Line: A novel, methicillin-resistant [corrected] Staphylococcus aureus clone (Uruguay clone) with a non-multidrug-resistant phenotype caused a large outbreak, including 7 deaths, in Montevideo, Uruguay.The clone was distinct from the highly virulent community clone represented by strain MW2, although both clones carried Panton-Valentine leukocidin gene and cna gene.

View Article: PubMed Central - PubMed

Affiliation: Juntendo University, Tokyo, Japan.

ABSTRACT
A novel, methicillin-resistant [corrected] Staphylococcus aureus clone (Uruguay clone) with a non-multidrug-resistant phenotype caused a large outbreak, including 7 deaths, in Montevideo, Uruguay. The clone was distinct from the highly virulent community clone represented by strain MW2, although both clones carried Panton-Valentine leukocidin gene and cna gene.

Show MeSH
Related in: MedlinePlus