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Cephalosporin and ciprofloxacin resistance in Salmonella, Taiwan.

Yan JJ, Chiou CS, Lauderdale TL, Tsai SH, Wu JJ - Emerging Infect. Dis. (2005)

Bottom Line: We report the prevalence and characteristics of Salmonella strains resistant to ciprofloxacin and extended-spectrum cephalosporins in Taiwan from January to May 2004.All isolates resistant to extended-spectrum cephalosporins carried blaCMY-2, and all ciprofloxacin-resistant Salmonella enterica serotype Choleraesuis isolates were genetically related.

View Article: PubMed Central - PubMed

Affiliation: National Cheng Kung University College of Medicine, Tainan, Taiwan.

ABSTRACT
We report the prevalence and characteristics of Salmonella strains resistant to ciprofloxacin and extended-spectrum cephalosporins in Taiwan from January to May 2004. All isolates resistant to extended-spectrum cephalosporins carried blaCMY-2, and all ciprofloxacin-resistant Salmonella enterica serotype Choleraesuis isolates were genetically related.

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EcoRI restriction patterns of transferred CMY-2–encoding plasmids of 18 Salmonella isolates. The result of the hybridization assay with the blaCMY-2 probe labeled with digoxigenin (Roche Molecular Biochemicals, Mannheim, Germany) is shown below the gel, and arrowheads indicate the locations of the restriction fragments that were hybridized. Lanes 2–21, plasmids from transconjugants of Salmonella isolates NB04.022, SB04.003, NL04.050, SA04.028, CG04.039, SG04.039, SG04.042, SE04.006, SG04.047, SE04.005, NG04.011, NG04.016, NG04.018, NC04.001, NC04.002, NC04.003, NC04.004, and CE04.015; lanes 1 and 22, molecular marker II (Roche Molecular Biochemicals); lanes 12 and 13, a 1-kb molecular marker (Promega Co., Madison, WI, USA).
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Figure 1: EcoRI restriction patterns of transferred CMY-2–encoding plasmids of 18 Salmonella isolates. The result of the hybridization assay with the blaCMY-2 probe labeled with digoxigenin (Roche Molecular Biochemicals, Mannheim, Germany) is shown below the gel, and arrowheads indicate the locations of the restriction fragments that were hybridized. Lanes 2–21, plasmids from transconjugants of Salmonella isolates NB04.022, SB04.003, NL04.050, SA04.028, CG04.039, SG04.039, SG04.042, SE04.006, SG04.047, SE04.005, NG04.011, NG04.016, NG04.018, NC04.001, NC04.002, NC04.003, NC04.004, and CE04.015; lanes 1 and 22, molecular marker II (Roche Molecular Biochemicals); lanes 12 and 13, a 1-kb molecular marker (Promega Co., Madison, WI, USA).

Mentions: ESC resistance was transferred from 18 of the 20 ESC-resistant Salmonella isolates to Escherichia coli C600 in the liquid mating-out assay (3,12). All transconjugants showed decreased susceptibilities to the 4 ESCs tested (MICs 16–64 μg/mL) and cefoxitin (MIC 64–128 μg/mL) and were susceptible to all non–β-lactam agents tested. A pI 9.0 vz β-lactamase and blaCMY-2 were detected by isoelectric focusing and PCR assays, respectively, in all transconjugants. Restricted by the endonuclease EcoRI, the 18 transferred plasmids produced 9 major restriction patterns (Figure 1 and Table 2). Patterns E and I were further divided into 4 and 2 subtypes, respectively. blaCMY-2 on the transferred plasmids was demonstrated by Southern hybridization with the blaCMY-2 probe.


Cephalosporin and ciprofloxacin resistance in Salmonella, Taiwan.

Yan JJ, Chiou CS, Lauderdale TL, Tsai SH, Wu JJ - Emerging Infect. Dis. (2005)

EcoRI restriction patterns of transferred CMY-2–encoding plasmids of 18 Salmonella isolates. The result of the hybridization assay with the blaCMY-2 probe labeled with digoxigenin (Roche Molecular Biochemicals, Mannheim, Germany) is shown below the gel, and arrowheads indicate the locations of the restriction fragments that were hybridized. Lanes 2–21, plasmids from transconjugants of Salmonella isolates NB04.022, SB04.003, NL04.050, SA04.028, CG04.039, SG04.039, SG04.042, SE04.006, SG04.047, SE04.005, NG04.011, NG04.016, NG04.018, NC04.001, NC04.002, NC04.003, NC04.004, and CE04.015; lanes 1 and 22, molecular marker II (Roche Molecular Biochemicals); lanes 12 and 13, a 1-kb molecular marker (Promega Co., Madison, WI, USA).
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Related In: Results  -  Collection

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Figure 1: EcoRI restriction patterns of transferred CMY-2–encoding plasmids of 18 Salmonella isolates. The result of the hybridization assay with the blaCMY-2 probe labeled with digoxigenin (Roche Molecular Biochemicals, Mannheim, Germany) is shown below the gel, and arrowheads indicate the locations of the restriction fragments that were hybridized. Lanes 2–21, plasmids from transconjugants of Salmonella isolates NB04.022, SB04.003, NL04.050, SA04.028, CG04.039, SG04.039, SG04.042, SE04.006, SG04.047, SE04.005, NG04.011, NG04.016, NG04.018, NC04.001, NC04.002, NC04.003, NC04.004, and CE04.015; lanes 1 and 22, molecular marker II (Roche Molecular Biochemicals); lanes 12 and 13, a 1-kb molecular marker (Promega Co., Madison, WI, USA).
Mentions: ESC resistance was transferred from 18 of the 20 ESC-resistant Salmonella isolates to Escherichia coli C600 in the liquid mating-out assay (3,12). All transconjugants showed decreased susceptibilities to the 4 ESCs tested (MICs 16–64 μg/mL) and cefoxitin (MIC 64–128 μg/mL) and were susceptible to all non–β-lactam agents tested. A pI 9.0 vz β-lactamase and blaCMY-2 were detected by isoelectric focusing and PCR assays, respectively, in all transconjugants. Restricted by the endonuclease EcoRI, the 18 transferred plasmids produced 9 major restriction patterns (Figure 1 and Table 2). Patterns E and I were further divided into 4 and 2 subtypes, respectively. blaCMY-2 on the transferred plasmids was demonstrated by Southern hybridization with the blaCMY-2 probe.

Bottom Line: We report the prevalence and characteristics of Salmonella strains resistant to ciprofloxacin and extended-spectrum cephalosporins in Taiwan from January to May 2004.All isolates resistant to extended-spectrum cephalosporins carried blaCMY-2, and all ciprofloxacin-resistant Salmonella enterica serotype Choleraesuis isolates were genetically related.

View Article: PubMed Central - PubMed

Affiliation: National Cheng Kung University College of Medicine, Tainan, Taiwan.

ABSTRACT
We report the prevalence and characteristics of Salmonella strains resistant to ciprofloxacin and extended-spectrum cephalosporins in Taiwan from January to May 2004. All isolates resistant to extended-spectrum cephalosporins carried blaCMY-2, and all ciprofloxacin-resistant Salmonella enterica serotype Choleraesuis isolates were genetically related.

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