Limits...
Tuberculosis due to resistant Haarlem strain, Tunisia.

Mardassi H, Namouchi A, Haltiti R, Zarrouk M, Mhenni B, Karboul A, Khabouchi N, Gey van Pittius NC, Streicher EM, Rauzier J, Gicquel B, Dellagi K - Emerging Infect. Dis. (2005)

Bottom Line: Multidrug-resistant tuberculosis was diagnosed in 21 HIV-negative, nonhospitalized male patients residing in northern Tunisia.A detailed investigation showed accelerated transmission of a Mycobacterium tuberculosis clone of the Haarlem type in 90% of all patients.This finding highlights the epidemic potential of this prevalent genotype.

View Article: PubMed Central - PubMed

Affiliation: Institut Pasteur de Tunis, Tunis-Belvédère, Tunisia. helmi.merdassi@pasteur.rns.tn

ABSTRACT
Multidrug-resistant tuberculosis was diagnosed in 21 HIV-negative, nonhospitalized male patients residing in northern Tunisia. A detailed investigation showed accelerated transmission of a Mycobacterium tuberculosis clone of the Haarlem type in 90% of all patients. This finding highlights the epidemic potential of this prevalent genotype.

Show MeSH

Related in: MedlinePlus

A) IS6110 restriction fragment length polymorphism (RFLP) analysis (left) and polymorphic GC-rich repetitive sequence (PGRS) typing (right) of patient P11. Lane 1, initial isolate; lane 2, follow-up isolate. B) IS6110 RFLP (left) and PGRS typing (right) of patient P20 (lane 1) compared with patient P3 (lane 2), a typical outbreak-associated patient. Lane M, reference strain MTB14323.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3367586&req=5

Figure 2: A) IS6110 restriction fragment length polymorphism (RFLP) analysis (left) and polymorphic GC-rich repetitive sequence (PGRS) typing (right) of patient P11. Lane 1, initial isolate; lane 2, follow-up isolate. B) IS6110 RFLP (left) and PGRS typing (right) of patient P20 (lane 1) compared with patient P3 (lane 2), a typical outbreak-associated patient. Lane M, reference strain MTB14323.

Mentions: As indicated in Table 1, with the exception of patient P20, the DNA samples subjected to molecular typing were obtained from the initial isolate of all new patients. RFLP showed that 18 patients had nearly identical IS6110 profiles (Figures 1 and 2). The predominant profile (occurring in 13 patients) showed 11 bands, while the remaining 5 patients had an additional IS6110 band. The presence or absence of the additional IS6110 band was not restricted to new or previously treated patients. The RFLP pattern of patient P11, a new patient, clearly showed a mixture of the 12-band profile and some additional IS6110 bands (Figure 2A). Typing of his follow-up culture, which was obtained after 6 months of directly observed short-course therapy, as recommended by the World Health Organization, yielded only the 12-band profile (Figure 2A). Laboratory records and epidemiologic data indicate that this patient likely had a dual infection.


Tuberculosis due to resistant Haarlem strain, Tunisia.

Mardassi H, Namouchi A, Haltiti R, Zarrouk M, Mhenni B, Karboul A, Khabouchi N, Gey van Pittius NC, Streicher EM, Rauzier J, Gicquel B, Dellagi K - Emerging Infect. Dis. (2005)

A) IS6110 restriction fragment length polymorphism (RFLP) analysis (left) and polymorphic GC-rich repetitive sequence (PGRS) typing (right) of patient P11. Lane 1, initial isolate; lane 2, follow-up isolate. B) IS6110 RFLP (left) and PGRS typing (right) of patient P20 (lane 1) compared with patient P3 (lane 2), a typical outbreak-associated patient. Lane M, reference strain MTB14323.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3367586&req=5

Figure 2: A) IS6110 restriction fragment length polymorphism (RFLP) analysis (left) and polymorphic GC-rich repetitive sequence (PGRS) typing (right) of patient P11. Lane 1, initial isolate; lane 2, follow-up isolate. B) IS6110 RFLP (left) and PGRS typing (right) of patient P20 (lane 1) compared with patient P3 (lane 2), a typical outbreak-associated patient. Lane M, reference strain MTB14323.
Mentions: As indicated in Table 1, with the exception of patient P20, the DNA samples subjected to molecular typing were obtained from the initial isolate of all new patients. RFLP showed that 18 patients had nearly identical IS6110 profiles (Figures 1 and 2). The predominant profile (occurring in 13 patients) showed 11 bands, while the remaining 5 patients had an additional IS6110 band. The presence or absence of the additional IS6110 band was not restricted to new or previously treated patients. The RFLP pattern of patient P11, a new patient, clearly showed a mixture of the 12-band profile and some additional IS6110 bands (Figure 2A). Typing of his follow-up culture, which was obtained after 6 months of directly observed short-course therapy, as recommended by the World Health Organization, yielded only the 12-band profile (Figure 2A). Laboratory records and epidemiologic data indicate that this patient likely had a dual infection.

Bottom Line: Multidrug-resistant tuberculosis was diagnosed in 21 HIV-negative, nonhospitalized male patients residing in northern Tunisia.A detailed investigation showed accelerated transmission of a Mycobacterium tuberculosis clone of the Haarlem type in 90% of all patients.This finding highlights the epidemic potential of this prevalent genotype.

View Article: PubMed Central - PubMed

Affiliation: Institut Pasteur de Tunis, Tunis-Belvédère, Tunisia. helmi.merdassi@pasteur.rns.tn

ABSTRACT
Multidrug-resistant tuberculosis was diagnosed in 21 HIV-negative, nonhospitalized male patients residing in northern Tunisia. A detailed investigation showed accelerated transmission of a Mycobacterium tuberculosis clone of the Haarlem type in 90% of all patients. This finding highlights the epidemic potential of this prevalent genotype.

Show MeSH
Related in: MedlinePlus