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Rifampicin resistance in tuberculosis outbreak, London, England.

Jenkins C - Emerging Infect. Dis. (2005)

Bottom Line: Mycobacterium tuberculosis isolates cultured from 6 patients associated with an isoniazid-resistant M. tuberculosis outbreak acquired rifampicin resistance.The rpoB gene sequence showed that resistance was associated with rare mutations in each isolate.Three isolates had a mutation outside the rifampicin resistance-determining region.

View Article: PubMed Central - PubMed

Affiliation: Royal Free Hospital, London, United Kingdom. claire.jenkins@royalfree.nhs.uk

ABSTRACT
Mycobacterium tuberculosis isolates cultured from 6 patients associated with an isoniazid-resistant M. tuberculosis outbreak acquired rifampicin resistance. The rpoB gene sequence showed that resistance was associated with rare mutations in each isolate. Three isolates had a mutation outside the rifampicin resistance-determining region.

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Alignment of rpoB gene sequences showing the mutations detected in this case study. WT, wildtype; T7, 018, 604 – V176F (G to T, highlighted in blue); 483, H526R (A to G, highlighted in yellow); 915, 317, S531W (C to G, highlighted in pink).
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Figure 1: Alignment of rpoB gene sequences showing the mutations detected in this case study. WT, wildtype; T7, 018, 604 – V176F (G to T, highlighted in blue); 483, H526R (A to G, highlighted in yellow); 915, 317, S531W (C to G, highlighted in pink).

Mentions: Analysis of the sequence data identified specific mutations in rpoB in all 6 strains studied (Figure 1). Three had mutations within the RRDR, and of these, 2 were C-to-G mutations at codon 456, inducing a serine to tryptophan amino acid conversion (S456W). The third was an A-to-G mutation at codon 451, resulting in a change from histidine to arginine (H451R) (Figure 1). No other mutations were identified in the DNA sequences outside the RRDR in these 3 isolates. The 3 other isolates had the mutation G to T at codon 176, outside the RRDR, which caused a change from valine to phenylalanine (V176F). Neither RRDR mutations nor any other mutations were found in the rpoB gene sequences of these 3 isolates.


Rifampicin resistance in tuberculosis outbreak, London, England.

Jenkins C - Emerging Infect. Dis. (2005)

Alignment of rpoB gene sequences showing the mutations detected in this case study. WT, wildtype; T7, 018, 604 – V176F (G to T, highlighted in blue); 483, H526R (A to G, highlighted in yellow); 915, 317, S531W (C to G, highlighted in pink).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3367579&req=5

Figure 1: Alignment of rpoB gene sequences showing the mutations detected in this case study. WT, wildtype; T7, 018, 604 – V176F (G to T, highlighted in blue); 483, H526R (A to G, highlighted in yellow); 915, 317, S531W (C to G, highlighted in pink).
Mentions: Analysis of the sequence data identified specific mutations in rpoB in all 6 strains studied (Figure 1). Three had mutations within the RRDR, and of these, 2 were C-to-G mutations at codon 456, inducing a serine to tryptophan amino acid conversion (S456W). The third was an A-to-G mutation at codon 451, resulting in a change from histidine to arginine (H451R) (Figure 1). No other mutations were identified in the DNA sequences outside the RRDR in these 3 isolates. The 3 other isolates had the mutation G to T at codon 176, outside the RRDR, which caused a change from valine to phenylalanine (V176F). Neither RRDR mutations nor any other mutations were found in the rpoB gene sequences of these 3 isolates.

Bottom Line: Mycobacterium tuberculosis isolates cultured from 6 patients associated with an isoniazid-resistant M. tuberculosis outbreak acquired rifampicin resistance.The rpoB gene sequence showed that resistance was associated with rare mutations in each isolate.Three isolates had a mutation outside the rifampicin resistance-determining region.

View Article: PubMed Central - PubMed

Affiliation: Royal Free Hospital, London, United Kingdom. claire.jenkins@royalfree.nhs.uk

ABSTRACT
Mycobacterium tuberculosis isolates cultured from 6 patients associated with an isoniazid-resistant M. tuberculosis outbreak acquired rifampicin resistance. The rpoB gene sequence showed that resistance was associated with rare mutations in each isolate. Three isolates had a mutation outside the rifampicin resistance-determining region.

Show MeSH
Related in: MedlinePlus