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Toscana virus in Spain.

Sanbonmatsu-Gámez S, Pérez-Ruiz M, Collao X, Sánchez-Seco MP, Morillas-Márquez F, de la Rosa-Fraile M, Navarro-Mari JM, Tenorio A - Emerging Infect. Dis. (2005)

Bottom Line: The overall seroprevalence rate was 24.9%, significantly increasing with age.Nucleotide sequence homology was 99%-100% in TOSV from vectors and patients and 80%-81% compared to the Italian strain ISS Phl.3.Sequencing of the N gene of TOSV isolates from patients and vectors indicated 87%-88% and 100% homology at the nucleotide and amino acid levels, respectively, compared to the Italian strain.

View Article: PubMed Central - PubMed

Affiliation: Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Avda. Fuerzas Armadas, s/n 18014-Granada, Spain.

ABSTRACT
Toscana virus (TOSV, Phlebovirus, family Bunyaviridae) infection is one of the most prevalent arboviruses in Spain. Within the objectives of a multidisciplinary network, a study on the epidemiology of TOSV was conducted in Granada, in southern Spain. The overall seroprevalence rate was 24.9%, significantly increasing with age. TOSV was detected in 3 of 103 sandfly pools by viral culture or reverse transcription-polymerase chain reaction from a region of the L gene. Nucleotide sequence homology was 99%-100% in TOSV from vectors and patients and 80%-81% compared to the Italian strain ISS Phl.3. Sequencing of the N gene of TOSV isolates from patients and vectors indicated 87%-88% and 100% homology at the nucleotide and amino acid levels, respectively, compared to the Italian strain. These findings demonstrate the circulation of at least 2 different lineages of TOSV in the Mediterranean basin, the Italian lineage and the Spanish lineage.

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Related in: MedlinePlus

Phylogenetic trees illustrating the relationship between representatives of different phleboviruses and the Spanish Toscana virus isolates within the nucleotide (nt, N) and deduced amino acids sequences (aa, N) of the N gene.
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Figure 3: Phylogenetic trees illustrating the relationship between representatives of different phleboviruses and the Spanish Toscana virus isolates within the nucleotide (nt, N) and deduced amino acids sequences (aa, N) of the N gene.

Mentions: Nucleotide and amino acid sequence homology within the N gene was ≈98%–100% among STIs from vectors and patients. Compared with the Italian strain, a 100% homology at the amino acid level was observed between STIs and the Italian strain. However, nucleotide sequences showed a 12%–13% difference between the strains (Table 4). The phylogenetic tree of nucleotide sequences shows that, within the TOSV group, STIs form a different cluster. However, at the amino acid level, both STIs and Italian TOSV strain group into a unique cluster (Figure 3).


Toscana virus in Spain.

Sanbonmatsu-Gámez S, Pérez-Ruiz M, Collao X, Sánchez-Seco MP, Morillas-Márquez F, de la Rosa-Fraile M, Navarro-Mari JM, Tenorio A - Emerging Infect. Dis. (2005)

Phylogenetic trees illustrating the relationship between representatives of different phleboviruses and the Spanish Toscana virus isolates within the nucleotide (nt, N) and deduced amino acids sequences (aa, N) of the N gene.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3367369&req=5

Figure 3: Phylogenetic trees illustrating the relationship between representatives of different phleboviruses and the Spanish Toscana virus isolates within the nucleotide (nt, N) and deduced amino acids sequences (aa, N) of the N gene.
Mentions: Nucleotide and amino acid sequence homology within the N gene was ≈98%–100% among STIs from vectors and patients. Compared with the Italian strain, a 100% homology at the amino acid level was observed between STIs and the Italian strain. However, nucleotide sequences showed a 12%–13% difference between the strains (Table 4). The phylogenetic tree of nucleotide sequences shows that, within the TOSV group, STIs form a different cluster. However, at the amino acid level, both STIs and Italian TOSV strain group into a unique cluster (Figure 3).

Bottom Line: The overall seroprevalence rate was 24.9%, significantly increasing with age.Nucleotide sequence homology was 99%-100% in TOSV from vectors and patients and 80%-81% compared to the Italian strain ISS Phl.3.Sequencing of the N gene of TOSV isolates from patients and vectors indicated 87%-88% and 100% homology at the nucleotide and amino acid levels, respectively, compared to the Italian strain.

View Article: PubMed Central - PubMed

Affiliation: Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Avda. Fuerzas Armadas, s/n 18014-Granada, Spain.

ABSTRACT
Toscana virus (TOSV, Phlebovirus, family Bunyaviridae) infection is one of the most prevalent arboviruses in Spain. Within the objectives of a multidisciplinary network, a study on the epidemiology of TOSV was conducted in Granada, in southern Spain. The overall seroprevalence rate was 24.9%, significantly increasing with age. TOSV was detected in 3 of 103 sandfly pools by viral culture or reverse transcription-polymerase chain reaction from a region of the L gene. Nucleotide sequence homology was 99%-100% in TOSV from vectors and patients and 80%-81% compared to the Italian strain ISS Phl.3. Sequencing of the N gene of TOSV isolates from patients and vectors indicated 87%-88% and 100% homology at the nucleotide and amino acid levels, respectively, compared to the Italian strain. These findings demonstrate the circulation of at least 2 different lineages of TOSV in the Mediterranean basin, the Italian lineage and the Spanish lineage.

Show MeSH
Related in: MedlinePlus