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Norovirus outbreaks from drinking water.

Maunula L, Miettinen IT, von Bonsdorff CH - Emerging Infect. Dis. (2005)

Bottom Line: As judged by RT-PCR results from patient samples, noroviruses caused 18 outbreaks.In 10 outbreaks, the water sample also yielded a norovirus.In all but 1 instance, the amplicon sequence was identical to that recovered from the patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine, HUCH Laboratory Diagnostics, University of Helsinki, 00014 Helsinki, Finland. Leena.Maunula@Helsinki.Fi

ABSTRACT
As part of an intensified monitoring program for foodborne disease outbreaks in Finland, waterborne outbreaks were investigated for viruses. The diagnostic procedure included analysis of patients' stool samples by electron microscopy and reverse transcription-polymerase chain reaction (RT-PCR) for noroviruses and astroviruses. When these test results were positive for a virus, the water sample was analyzed. Virus concentration was based on positively charged filters from 1-L samples. Of the total 41 waterborne outbreaks reported during the observation period (1998-2003), samples from 28 outbreaks were available for analysis. As judged by RT-PCR results from patient samples, noroviruses caused 18 outbreaks. In 10 outbreaks, the water sample also yielded a norovirus. In all but 1 instance, the amplicon sequence was identical to that recovered from the patients. The ubiquity of waterborne norovirus outbreaks calls for measures to monitor water for viruses.

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Related in: MedlinePlus

Monthly distribution of waterborne outbreaks, including norovirus outbreaks, Finland, 1998–2003.
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Figure 2: Monthly distribution of waterborne outbreaks, including norovirus outbreaks, Finland, 1998–2003.

Mentions: The geographic distribution of the waterborne norovirus outbreaks is shown in Figure 1. Outbreaks occurred all over the country, from the southern archipelago to the northernmost parts of Finland. Seasonal risk for waterborne norovirus outbreak seemed to be approximately equal (Figure 2). Half (20 of 41) of the waterborne epidemics occurred in summer, and norovirus outbreaks (11 of 15) were most common in late winter to spring (February–May). In fact most outbreaks in winter were caused by noroviruses, while in summer they were mainly caused by bacteria.


Norovirus outbreaks from drinking water.

Maunula L, Miettinen IT, von Bonsdorff CH - Emerging Infect. Dis. (2005)

Monthly distribution of waterborne outbreaks, including norovirus outbreaks, Finland, 1998–2003.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3367355&req=5

Figure 2: Monthly distribution of waterborne outbreaks, including norovirus outbreaks, Finland, 1998–2003.
Mentions: The geographic distribution of the waterborne norovirus outbreaks is shown in Figure 1. Outbreaks occurred all over the country, from the southern archipelago to the northernmost parts of Finland. Seasonal risk for waterborne norovirus outbreak seemed to be approximately equal (Figure 2). Half (20 of 41) of the waterborne epidemics occurred in summer, and norovirus outbreaks (11 of 15) were most common in late winter to spring (February–May). In fact most outbreaks in winter were caused by noroviruses, while in summer they were mainly caused by bacteria.

Bottom Line: As judged by RT-PCR results from patient samples, noroviruses caused 18 outbreaks.In 10 outbreaks, the water sample also yielded a norovirus.In all but 1 instance, the amplicon sequence was identical to that recovered from the patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine, HUCH Laboratory Diagnostics, University of Helsinki, 00014 Helsinki, Finland. Leena.Maunula@Helsinki.Fi

ABSTRACT
As part of an intensified monitoring program for foodborne disease outbreaks in Finland, waterborne outbreaks were investigated for viruses. The diagnostic procedure included analysis of patients' stool samples by electron microscopy and reverse transcription-polymerase chain reaction (RT-PCR) for noroviruses and astroviruses. When these test results were positive for a virus, the water sample was analyzed. Virus concentration was based on positively charged filters from 1-L samples. Of the total 41 waterborne outbreaks reported during the observation period (1998-2003), samples from 28 outbreaks were available for analysis. As judged by RT-PCR results from patient samples, noroviruses caused 18 outbreaks. In 10 outbreaks, the water sample also yielded a norovirus. In all but 1 instance, the amplicon sequence was identical to that recovered from the patients. The ubiquity of waterborne norovirus outbreaks calls for measures to monitor water for viruses.

Show MeSH
Related in: MedlinePlus