Limits...
Tickborne pathogen detection, Western Siberia, Russia.

Rar VA, Fomenko NV, Dobrotvorsky AK, Livanova NN, Rudakova SA, Fedorov EG, Astanin VB, Morozova OV - Emerging Infect. Dis. (2005)

Bottom Line: D. reticulatus ticks contained DNA of B. burgdorferi sensu lato (3.6% +/- 2.0%), Bartonella spp. (21.4% +/- 4.5%), and Babesia canis canis (3.6% +/- 2.0%).Borrelia garinii, Borrelia afzelii, and their mixed infections were observed among I. persulcatus, whereas B. garinii NT29 DNA was seen in samples from D. reticulatus.Among the I. persulcatus ticks studied, no Babesia spp. were observed, whereas B. canis canis was the single subspecies found in D. reticulatus.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch--Russian Academy of Sciences, Lavrentyev's Avenue 8, 630090 Novosibirsk, Russia.

ABSTRACT
Ixodes persulcatus (n = 125) and Dermacentor reticulatus (n = 84) ticks from Western Siberia, Russia, were tested for infection with Borrelia, Anaplasma/Ehrlichia, Bartonella, and Babesia spp. by using nested polymerase chain reaction assays with subsequent sequencing. I. persulcatus ticks were infected with Borrelia burgdorferi sensu lato (37.6% +/- 4.3% [standard deviation]), Anaplasma phagocytophilum (2.4% +/- 1.4%), Ehrlichia muris (8.8% +/- 2.5%), and Bartonella spp. (37.6% +/- 4.3%). D. reticulatus ticks contained DNA of B. burgdorferi sensu lato (3.6% +/- 2.0%), Bartonella spp. (21.4% +/- 4.5%), and Babesia canis canis (3.6% +/- 2.0%). Borrelia garinii, Borrelia afzelii, and their mixed infections were observed among I. persulcatus, whereas B. garinii NT29 DNA was seen in samples from D. reticulatus. Among the I. persulcatus ticks studied, no Babesia spp. were observed, whereas B. canis canis was the single subspecies found in D. reticulatus.

Show MeSH

Related in: MedlinePlus

Phylogenetic tree based on the Bartonella groEL gene fragment sequences. Scale bar indicates an evolutionary distance of 0.02 nucleotides per position in the sequence. Bartonella bacilliformis was used as outgroup. Numbers above the branches indicate bootstrap support indexes. Samples from Ixodes persulcatus (Ip) and Dermacentor reticulatus (Dr) from this study are in boldface.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3367347&req=5

Figure 4: Phylogenetic tree based on the Bartonella groEL gene fragment sequences. Scale bar indicates an evolutionary distance of 0.02 nucleotides per position in the sequence. Bartonella bacilliformis was used as outgroup. Numbers above the branches indicate bootstrap support indexes. Samples from Ixodes persulcatus (Ip) and Dermacentor reticulatus (Dr) from this study are in boldface.

Mentions: Bartonella DNA was detected by using nested PCR with primers that corresponded to the groEL gene in 47 I. persulcatus and 18 D. reticulatus ticks (Table). Comparative analysis of the groEL gene fragment nucleotide sequences of 190 bp showed 2 species, B. henselae and B. quintana, in both tick species. Part of the data is shown in Figure 4. The evidently separated 2 clades, B. henselae and B. quintana, were monophyletic with good statistical support (99% and 90%, respectively).


Tickborne pathogen detection, Western Siberia, Russia.

Rar VA, Fomenko NV, Dobrotvorsky AK, Livanova NN, Rudakova SA, Fedorov EG, Astanin VB, Morozova OV - Emerging Infect. Dis. (2005)

Phylogenetic tree based on the Bartonella groEL gene fragment sequences. Scale bar indicates an evolutionary distance of 0.02 nucleotides per position in the sequence. Bartonella bacilliformis was used as outgroup. Numbers above the branches indicate bootstrap support indexes. Samples from Ixodes persulcatus (Ip) and Dermacentor reticulatus (Dr) from this study are in boldface.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3367347&req=5

Figure 4: Phylogenetic tree based on the Bartonella groEL gene fragment sequences. Scale bar indicates an evolutionary distance of 0.02 nucleotides per position in the sequence. Bartonella bacilliformis was used as outgroup. Numbers above the branches indicate bootstrap support indexes. Samples from Ixodes persulcatus (Ip) and Dermacentor reticulatus (Dr) from this study are in boldface.
Mentions: Bartonella DNA was detected by using nested PCR with primers that corresponded to the groEL gene in 47 I. persulcatus and 18 D. reticulatus ticks (Table). Comparative analysis of the groEL gene fragment nucleotide sequences of 190 bp showed 2 species, B. henselae and B. quintana, in both tick species. Part of the data is shown in Figure 4. The evidently separated 2 clades, B. henselae and B. quintana, were monophyletic with good statistical support (99% and 90%, respectively).

Bottom Line: D. reticulatus ticks contained DNA of B. burgdorferi sensu lato (3.6% +/- 2.0%), Bartonella spp. (21.4% +/- 4.5%), and Babesia canis canis (3.6% +/- 2.0%).Borrelia garinii, Borrelia afzelii, and their mixed infections were observed among I. persulcatus, whereas B. garinii NT29 DNA was seen in samples from D. reticulatus.Among the I. persulcatus ticks studied, no Babesia spp. were observed, whereas B. canis canis was the single subspecies found in D. reticulatus.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch--Russian Academy of Sciences, Lavrentyev's Avenue 8, 630090 Novosibirsk, Russia.

ABSTRACT
Ixodes persulcatus (n = 125) and Dermacentor reticulatus (n = 84) ticks from Western Siberia, Russia, were tested for infection with Borrelia, Anaplasma/Ehrlichia, Bartonella, and Babesia spp. by using nested polymerase chain reaction assays with subsequent sequencing. I. persulcatus ticks were infected with Borrelia burgdorferi sensu lato (37.6% +/- 4.3% [standard deviation]), Anaplasma phagocytophilum (2.4% +/- 1.4%), Ehrlichia muris (8.8% +/- 2.5%), and Bartonella spp. (37.6% +/- 4.3%). D. reticulatus ticks contained DNA of B. burgdorferi sensu lato (3.6% +/- 2.0%), Bartonella spp. (21.4% +/- 4.5%), and Babesia canis canis (3.6% +/- 2.0%). Borrelia garinii, Borrelia afzelii, and their mixed infections were observed among I. persulcatus, whereas B. garinii NT29 DNA was seen in samples from D. reticulatus. Among the I. persulcatus ticks studied, no Babesia spp. were observed, whereas B. canis canis was the single subspecies found in D. reticulatus.

Show MeSH
Related in: MedlinePlus