Limits...
Tickborne pathogen detection, Western Siberia, Russia.

Rar VA, Fomenko NV, Dobrotvorsky AK, Livanova NN, Rudakova SA, Fedorov EG, Astanin VB, Morozova OV - Emerging Infect. Dis. (2005)

Bottom Line: D. reticulatus ticks contained DNA of B. burgdorferi sensu lato (3.6% +/- 2.0%), Bartonella spp. (21.4% +/- 4.5%), and Babesia canis canis (3.6% +/- 2.0%).Borrelia garinii, Borrelia afzelii, and their mixed infections were observed among I. persulcatus, whereas B. garinii NT29 DNA was seen in samples from D. reticulatus.Among the I. persulcatus ticks studied, no Babesia spp. were observed, whereas B. canis canis was the single subspecies found in D. reticulatus.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch--Russian Academy of Sciences, Lavrentyev's Avenue 8, 630090 Novosibirsk, Russia.

ABSTRACT
Ixodes persulcatus (n = 125) and Dermacentor reticulatus (n = 84) ticks from Western Siberia, Russia, were tested for infection with Borrelia, Anaplasma/Ehrlichia, Bartonella, and Babesia spp. by using nested polymerase chain reaction assays with subsequent sequencing. I. persulcatus ticks were infected with Borrelia burgdorferi sensu lato (37.6% +/- 4.3% [standard deviation]), Anaplasma phagocytophilum (2.4% +/- 1.4%), Ehrlichia muris (8.8% +/- 2.5%), and Bartonella spp. (37.6% +/- 4.3%). D. reticulatus ticks contained DNA of B. burgdorferi sensu lato (3.6% +/- 2.0%), Bartonella spp. (21.4% +/- 4.5%), and Babesia canis canis (3.6% +/- 2.0%). Borrelia garinii, Borrelia afzelii, and their mixed infections were observed among I. persulcatus, whereas B. garinii NT29 DNA was seen in samples from D. reticulatus. Among the I. persulcatus ticks studied, no Babesia spp. were observed, whereas B. canis canis was the single subspecies found in D. reticulatus.

Show MeSH

Related in: MedlinePlus

Phylogenetic tree based on the Borrelia burgdorferi sensu lato 5S-23S rRNA intergenic spacer fragment sequences. Scale bar indicates an evolutionary distance of 0.02 nucleotides per position in the sequence. Borrelia andersonii was used as outgroup. Numbers above the branches indicate bootstrap support indexes. Samples isolated from Ixodes persulcatus (Ip) and Dermacentor reticulatus (Dr) in this research are in boldface.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3367347&req=5

Figure 2: Phylogenetic tree based on the Borrelia burgdorferi sensu lato 5S-23S rRNA intergenic spacer fragment sequences. Scale bar indicates an evolutionary distance of 0.02 nucleotides per position in the sequence. Borrelia andersonii was used as outgroup. Numbers above the branches indicate bootstrap support indexes. Samples isolated from Ixodes persulcatus (Ip) and Dermacentor reticulatus (Dr) in this research are in boldface.

Mentions: Nucleotide sequences of PCR products determined in this study were analyzed by BlastN and aligned with ClustalW (29). Phylogenetic analysis was performed with MEGA 3.0 software (30). We used the unweighted pair-group method with arithmetic mean (UPGMA) and neighbor-joining algorithms with the Kimura 2-parameter model to generate the distance matrix as well as maximum parsimony and minimal evolution with a heuristic search. Bootstrap analysis was performed with 1,000 replications. GenBank accession numbers for the sequences used in the phylogenetic analysis are shown in Figures 2–5.


Tickborne pathogen detection, Western Siberia, Russia.

Rar VA, Fomenko NV, Dobrotvorsky AK, Livanova NN, Rudakova SA, Fedorov EG, Astanin VB, Morozova OV - Emerging Infect. Dis. (2005)

Phylogenetic tree based on the Borrelia burgdorferi sensu lato 5S-23S rRNA intergenic spacer fragment sequences. Scale bar indicates an evolutionary distance of 0.02 nucleotides per position in the sequence. Borrelia andersonii was used as outgroup. Numbers above the branches indicate bootstrap support indexes. Samples isolated from Ixodes persulcatus (Ip) and Dermacentor reticulatus (Dr) in this research are in boldface.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3367347&req=5

Figure 2: Phylogenetic tree based on the Borrelia burgdorferi sensu lato 5S-23S rRNA intergenic spacer fragment sequences. Scale bar indicates an evolutionary distance of 0.02 nucleotides per position in the sequence. Borrelia andersonii was used as outgroup. Numbers above the branches indicate bootstrap support indexes. Samples isolated from Ixodes persulcatus (Ip) and Dermacentor reticulatus (Dr) in this research are in boldface.
Mentions: Nucleotide sequences of PCR products determined in this study were analyzed by BlastN and aligned with ClustalW (29). Phylogenetic analysis was performed with MEGA 3.0 software (30). We used the unweighted pair-group method with arithmetic mean (UPGMA) and neighbor-joining algorithms with the Kimura 2-parameter model to generate the distance matrix as well as maximum parsimony and minimal evolution with a heuristic search. Bootstrap analysis was performed with 1,000 replications. GenBank accession numbers for the sequences used in the phylogenetic analysis are shown in Figures 2–5.

Bottom Line: D. reticulatus ticks contained DNA of B. burgdorferi sensu lato (3.6% +/- 2.0%), Bartonella spp. (21.4% +/- 4.5%), and Babesia canis canis (3.6% +/- 2.0%).Borrelia garinii, Borrelia afzelii, and their mixed infections were observed among I. persulcatus, whereas B. garinii NT29 DNA was seen in samples from D. reticulatus.Among the I. persulcatus ticks studied, no Babesia spp. were observed, whereas B. canis canis was the single subspecies found in D. reticulatus.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch--Russian Academy of Sciences, Lavrentyev's Avenue 8, 630090 Novosibirsk, Russia.

ABSTRACT
Ixodes persulcatus (n = 125) and Dermacentor reticulatus (n = 84) ticks from Western Siberia, Russia, were tested for infection with Borrelia, Anaplasma/Ehrlichia, Bartonella, and Babesia spp. by using nested polymerase chain reaction assays with subsequent sequencing. I. persulcatus ticks were infected with Borrelia burgdorferi sensu lato (37.6% +/- 4.3% [standard deviation]), Anaplasma phagocytophilum (2.4% +/- 1.4%), Ehrlichia muris (8.8% +/- 2.5%), and Bartonella spp. (37.6% +/- 4.3%). D. reticulatus ticks contained DNA of B. burgdorferi sensu lato (3.6% +/- 2.0%), Bartonella spp. (21.4% +/- 4.5%), and Babesia canis canis (3.6% +/- 2.0%). Borrelia garinii, Borrelia afzelii, and their mixed infections were observed among I. persulcatus, whereas B. garinii NT29 DNA was seen in samples from D. reticulatus. Among the I. persulcatus ticks studied, no Babesia spp. were observed, whereas B. canis canis was the single subspecies found in D. reticulatus.

Show MeSH
Related in: MedlinePlus