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Diversity in the enteric viruses detected in outbreaks of gastroenteritis from Mumbai, Western India.

Chitambar S, Gopalkrishna V, Chhabra P, Patil P, Verma H, Lahon A, Arora R, Tatte V, Ranshing S, Dhale G, Kolhapure R, Tikute S, Kulkarni J, Bhardwaj R, Akarte S, Pawar S - Int J Environ Res Public Health (2012)

Bottom Line: Single viral infections in both, March and October showed predominance of enterovirus (EV, 33.3% and 40%) and rotavirus A (RVA, 33.3% and 25%).VP1/2A junction region based genotyping showed presence of 11 different serotypes of EVs.Although no virus was detected in the tested water samples, examination of both water and sewage pipelines in gastroenteritis affected localities indicated leakages and possibility of contamination of drinking water with sewage water.

View Article: PubMed Central - PubMed

Affiliation: Enteric Viruses Group, National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411001, India. drshobha.niv@gmail.com

ABSTRACT
Faecal specimens collected from two outbreaks of acute gastroenteritis that occurred in southern Mumbai, India in March and October, 2006 were tested for seven different enteric viruses. Among the 218 specimens tested, 95 (43.6%) were positive, 73 (76.8%) for a single virus and 22 (23.2%) for multiple viruses. Single viral infections in both, March and October showed predominance of enterovirus (EV, 33.3% and 40%) and rotavirus A (RVA, 33.3% and 25%). The other viruses detected in these months were norovirus (NoV, 12.1% and 10%), rotavirus B (RVB, 12.1% and 10%), enteric adenovirus (AdV, 6.1% and 7.5%), Aichivirus (AiV, 3% and 7.5%) and human astrovirus (HAstV, 3% and 0%). Mixed viral infections were largely represented by two viruses (84.6% and 88.9%), a small proportion showed presence of three (7.7% and 11%) and four (7.7% and 0%) viruses in the two outbreaks. Genotyping of the viruses revealed predominance of RVA G2P[4], RVB G2 (Indian Bangladeshi lineage), NoV GII.4, AdV-40, HAstV-8 and AiV B types. VP1/2A junction region based genotyping showed presence of 11 different serotypes of EVs. Although no virus was detected in the tested water samples, examination of both water and sewage pipelines in gastroenteritis affected localities indicated leakages and possibility of contamination of drinking water with sewage water. Coexistence of multiple enteric viruses during the two outbreaks of gastroenteritis emphasizes the need to expand such investigations to other parts of India.

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Related in: MedlinePlus

Frequencies of admission of patients with gastroenteritis referred to GTH and JJH in the year 2006. * includes 66 children, 12 adolescents and 222 adults hospitalized during 13th–26th March, 2006; ** includes 53 children, 13 adolescents and 198 adults hospitalized during 5th–15th October, 2006.
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ijerph-09-00895-f001: Frequencies of admission of patients with gastroenteritis referred to GTH and JJH in the year 2006. * includes 66 children, 12 adolescents and 222 adults hospitalized during 13th–26th March, 2006; ** includes 53 children, 13 adolescents and 198 adults hospitalized during 5th–15th October, 2006.

Mentions: Two outbreaks of acute gastroenteritis were reported in the months of March and October 2006, respectively, from two and one localities of southern Mumbai. The outbreak affected localities, each 1–1.5 km2 in area that was occupied by 4500 and 7000 inhabitants respectively. Nearly 7% and 4% of the inhabitants were affected by the disease, respectively, during the months of March and October, 2006. In order to establish the incidence of gastroenteritis outbreaks, the number of gastroenteritis cases admitted monthly to GTH and JJH was recorded for the year, 2006. Figure 1 shows that the frequencies of admission for gastroenteritis in the hospitals were increased remarkably in GTH and JJH in March and October, 2006 respectively. The records of both hospitals also showed higher number of admissions in 2006 [886 (GTH), 1322 (JJH)] as compared to those in 2005 [278 (GTH), 477 (JJH)].


Diversity in the enteric viruses detected in outbreaks of gastroenteritis from Mumbai, Western India.

Chitambar S, Gopalkrishna V, Chhabra P, Patil P, Verma H, Lahon A, Arora R, Tatte V, Ranshing S, Dhale G, Kolhapure R, Tikute S, Kulkarni J, Bhardwaj R, Akarte S, Pawar S - Int J Environ Res Public Health (2012)

Frequencies of admission of patients with gastroenteritis referred to GTH and JJH in the year 2006. * includes 66 children, 12 adolescents and 222 adults hospitalized during 13th–26th March, 2006; ** includes 53 children, 13 adolescents and 198 adults hospitalized during 5th–15th October, 2006.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3367285&req=5

ijerph-09-00895-f001: Frequencies of admission of patients with gastroenteritis referred to GTH and JJH in the year 2006. * includes 66 children, 12 adolescents and 222 adults hospitalized during 13th–26th March, 2006; ** includes 53 children, 13 adolescents and 198 adults hospitalized during 5th–15th October, 2006.
Mentions: Two outbreaks of acute gastroenteritis were reported in the months of March and October 2006, respectively, from two and one localities of southern Mumbai. The outbreak affected localities, each 1–1.5 km2 in area that was occupied by 4500 and 7000 inhabitants respectively. Nearly 7% and 4% of the inhabitants were affected by the disease, respectively, during the months of March and October, 2006. In order to establish the incidence of gastroenteritis outbreaks, the number of gastroenteritis cases admitted monthly to GTH and JJH was recorded for the year, 2006. Figure 1 shows that the frequencies of admission for gastroenteritis in the hospitals were increased remarkably in GTH and JJH in March and October, 2006 respectively. The records of both hospitals also showed higher number of admissions in 2006 [886 (GTH), 1322 (JJH)] as compared to those in 2005 [278 (GTH), 477 (JJH)].

Bottom Line: Single viral infections in both, March and October showed predominance of enterovirus (EV, 33.3% and 40%) and rotavirus A (RVA, 33.3% and 25%).VP1/2A junction region based genotyping showed presence of 11 different serotypes of EVs.Although no virus was detected in the tested water samples, examination of both water and sewage pipelines in gastroenteritis affected localities indicated leakages and possibility of contamination of drinking water with sewage water.

View Article: PubMed Central - PubMed

Affiliation: Enteric Viruses Group, National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411001, India. drshobha.niv@gmail.com

ABSTRACT
Faecal specimens collected from two outbreaks of acute gastroenteritis that occurred in southern Mumbai, India in March and October, 2006 were tested for seven different enteric viruses. Among the 218 specimens tested, 95 (43.6%) were positive, 73 (76.8%) for a single virus and 22 (23.2%) for multiple viruses. Single viral infections in both, March and October showed predominance of enterovirus (EV, 33.3% and 40%) and rotavirus A (RVA, 33.3% and 25%). The other viruses detected in these months were norovirus (NoV, 12.1% and 10%), rotavirus B (RVB, 12.1% and 10%), enteric adenovirus (AdV, 6.1% and 7.5%), Aichivirus (AiV, 3% and 7.5%) and human astrovirus (HAstV, 3% and 0%). Mixed viral infections were largely represented by two viruses (84.6% and 88.9%), a small proportion showed presence of three (7.7% and 11%) and four (7.7% and 0%) viruses in the two outbreaks. Genotyping of the viruses revealed predominance of RVA G2P[4], RVB G2 (Indian Bangladeshi lineage), NoV GII.4, AdV-40, HAstV-8 and AiV B types. VP1/2A junction region based genotyping showed presence of 11 different serotypes of EVs. Although no virus was detected in the tested water samples, examination of both water and sewage pipelines in gastroenteritis affected localities indicated leakages and possibility of contamination of drinking water with sewage water. Coexistence of multiple enteric viruses during the two outbreaks of gastroenteritis emphasizes the need to expand such investigations to other parts of India.

Show MeSH
Related in: MedlinePlus