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Geographical variation in antibiotic-resistant Escherichia coli isolates from stool, cow-dung and drinking water.

Sahoo KC, Tamhankar AJ, Sahoo S, Sahu PS, Klintz SR, Lundborg CS - Int J Environ Res Public Health (2012)

Bottom Line: Resistance to at least one antibiotic was detected in 90% or more of the E. coli isolates.Ciprofloxacin MIC values ranged from 8 to 32 µg/mL.Similarly, the co-resistance in cow-dung (OR = 2.5, 95% CI 1.39-4.37, P = 0.002) and drinking water (OR = 3.2, 95% CI 1.36-7.41, P = 0.008) as well as the multi-resistance in cow-dung (OR = 2.2, 95% CI 1.12-4.34, P = 0.022) and drinking water (OR = 2.7, 95% CI 1.06-7.07, P = 0.036) were also higher in the non-coastal compared to the coastal region.

View Article: PubMed Central - PubMed

Affiliation: Division of Global Health (IHCAR), Department of Public Health Sciences, Karolinska Institutet, Nobels väg 9, SE 171 77 Stockholm, Sweden. sahookrushna@yahoo.com

ABSTRACT
Little information is available on relationships between the biophysical environment and antibiotic resistance. This study was conducted to investigate the antibiotic resistance pattern of Escherichia coli isolated from child stool samples, cow-dung and drinking water from the non-coastal (230 households) and coastal (187 households) regions of Odisha, India. Susceptibility testing of E. coli isolates (n = 696) to the following antibiotics: tetracycline, ampicillin/sulbactam, cefuroxime, cefotaxime, cefixime, cotrimoxazole, amikacin, ciprofloxacin, norfloxacin and nalidixic acid was performed by the disk diffusion method. Ciprofloxacin minimum inhibitory concentration (MIC) values were determined for ciprofloxacin-resistant isolates (n = 83). Resistance to at least one antibiotic was detected in 90% or more of the E. coli isolates. Ciprofloxacin MIC values ranged from 8 to 32 µg/mL. The odds ratio (OR) of resistance in E. coli isolates from children's stool (OR = 3.1, 95% CI 1.18-8.01), cow-dung (OR = 3.6, 95% CI 1.59-8.03, P = 0.002) and drinking water (OR = 3.8, 95% CI 1.00-14.44, P = 0.049) were higher in non-coastal compared to coastal region. Similarly, the co-resistance in cow-dung (OR = 2.5, 95% CI 1.39-4.37, P = 0.002) and drinking water (OR = 3.2, 95% CI 1.36-7.41, P = 0.008) as well as the multi-resistance in cow-dung (OR = 2.2, 95% CI 1.12-4.34, P = 0.022) and drinking water (OR = 2.7, 95% CI 1.06-7.07, P = 0.036) were also higher in the non-coastal compared to the coastal region.

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Ciprofloxacin MIC (Minimum inhibitory concentration) values among ciprofloxacin-resistant E. coli isolates from (a) Children’s stools samples (n = 39); (b) Cow-dung samples (n = 27); (c) Drinking water samples (n = 17); and (d) Total number of isolates (n = 83).
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ijerph-09-00746-f001: Ciprofloxacin MIC (Minimum inhibitory concentration) values among ciprofloxacin-resistant E. coli isolates from (a) Children’s stools samples (n = 39); (b) Cow-dung samples (n = 27); (c) Drinking water samples (n = 17); and (d) Total number of isolates (n = 83).

Mentions: Ciprofloxacin MIC values ranged from 8 to 32 μg/mL, regardless of type of sample and geographic location, as shown in Figure 1. E. coli isolates from children’s stools had significantly higher levels of resistance (≥24 µg/mL, P = 0.025) when compared to cow-dung and drinking water isolates. Amongst cow-dung E. coli isolates, the highest level of resistance (≥32 µg/mL) was observed in isolates from the non-coastal region only. Overall, E. coli isolates originating from the non-coastal region tended to have higher levels of resistance as compared to those from the coastal region, albeit not statistically significant.


Geographical variation in antibiotic-resistant Escherichia coli isolates from stool, cow-dung and drinking water.

Sahoo KC, Tamhankar AJ, Sahoo S, Sahu PS, Klintz SR, Lundborg CS - Int J Environ Res Public Health (2012)

Ciprofloxacin MIC (Minimum inhibitory concentration) values among ciprofloxacin-resistant E. coli isolates from (a) Children’s stools samples (n = 39); (b) Cow-dung samples (n = 27); (c) Drinking water samples (n = 17); and (d) Total number of isolates (n = 83).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3367274&req=5

ijerph-09-00746-f001: Ciprofloxacin MIC (Minimum inhibitory concentration) values among ciprofloxacin-resistant E. coli isolates from (a) Children’s stools samples (n = 39); (b) Cow-dung samples (n = 27); (c) Drinking water samples (n = 17); and (d) Total number of isolates (n = 83).
Mentions: Ciprofloxacin MIC values ranged from 8 to 32 μg/mL, regardless of type of sample and geographic location, as shown in Figure 1. E. coli isolates from children’s stools had significantly higher levels of resistance (≥24 µg/mL, P = 0.025) when compared to cow-dung and drinking water isolates. Amongst cow-dung E. coli isolates, the highest level of resistance (≥32 µg/mL) was observed in isolates from the non-coastal region only. Overall, E. coli isolates originating from the non-coastal region tended to have higher levels of resistance as compared to those from the coastal region, albeit not statistically significant.

Bottom Line: Resistance to at least one antibiotic was detected in 90% or more of the E. coli isolates.Ciprofloxacin MIC values ranged from 8 to 32 µg/mL.Similarly, the co-resistance in cow-dung (OR = 2.5, 95% CI 1.39-4.37, P = 0.002) and drinking water (OR = 3.2, 95% CI 1.36-7.41, P = 0.008) as well as the multi-resistance in cow-dung (OR = 2.2, 95% CI 1.12-4.34, P = 0.022) and drinking water (OR = 2.7, 95% CI 1.06-7.07, P = 0.036) were also higher in the non-coastal compared to the coastal region.

View Article: PubMed Central - PubMed

Affiliation: Division of Global Health (IHCAR), Department of Public Health Sciences, Karolinska Institutet, Nobels väg 9, SE 171 77 Stockholm, Sweden. sahookrushna@yahoo.com

ABSTRACT
Little information is available on relationships between the biophysical environment and antibiotic resistance. This study was conducted to investigate the antibiotic resistance pattern of Escherichia coli isolated from child stool samples, cow-dung and drinking water from the non-coastal (230 households) and coastal (187 households) regions of Odisha, India. Susceptibility testing of E. coli isolates (n = 696) to the following antibiotics: tetracycline, ampicillin/sulbactam, cefuroxime, cefotaxime, cefixime, cotrimoxazole, amikacin, ciprofloxacin, norfloxacin and nalidixic acid was performed by the disk diffusion method. Ciprofloxacin minimum inhibitory concentration (MIC) values were determined for ciprofloxacin-resistant isolates (n = 83). Resistance to at least one antibiotic was detected in 90% or more of the E. coli isolates. Ciprofloxacin MIC values ranged from 8 to 32 µg/mL. The odds ratio (OR) of resistance in E. coli isolates from children's stool (OR = 3.1, 95% CI 1.18-8.01), cow-dung (OR = 3.6, 95% CI 1.59-8.03, P = 0.002) and drinking water (OR = 3.8, 95% CI 1.00-14.44, P = 0.049) were higher in non-coastal compared to coastal region. Similarly, the co-resistance in cow-dung (OR = 2.5, 95% CI 1.39-4.37, P = 0.002) and drinking water (OR = 3.2, 95% CI 1.36-7.41, P = 0.008) as well as the multi-resistance in cow-dung (OR = 2.2, 95% CI 1.12-4.34, P = 0.022) and drinking water (OR = 2.7, 95% CI 1.06-7.07, P = 0.036) were also higher in the non-coastal compared to the coastal region.

Show MeSH
Related in: MedlinePlus