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High-fat diet transition reduces brain DHA levels associated with altered brain plasticity and behaviour.

Sharma S, Zhuang Y, Gomez-Pinilla F - Sci Rep (2012)

Bottom Line: To assess how the shift from a healthy diet rich in omega-3 fatty acids to a diet rich in saturated fatty acid affects the substrates for brain plasticity and function, we used pregnant rats fed with omega-3 supplemented diet from their 2nd day of gestation period as well as their male pups for 12 weeks.We found that the HFD increased vulnerability for anxiety-like behavior, and that these modifications harmonized with changes in the anxiety-related NPY1 receptor and the reduced levels of BDNF, and its signalling receptor pTrkB, as well as the CREB protein.Brain DHA contents were significantly associated with the levels of anxiety-like behavior in these rats.

View Article: PubMed Central - PubMed

Affiliation: Dept. Integrative Biology & Physiology, UCLA, Los Angeles, CA 90095, USA.

ABSTRACT
To assess how the shift from a healthy diet rich in omega-3 fatty acids to a diet rich in saturated fatty acid affects the substrates for brain plasticity and function, we used pregnant rats fed with omega-3 supplemented diet from their 2nd day of gestation period as well as their male pups for 12 weeks. Afterwards, the animals were randomly assigned to either a group fed on the same diet or a group fed on a high-fat diet (HFD) rich in saturated fats for 3 weeks. We found that the HFD increased vulnerability for anxiety-like behavior, and that these modifications harmonized with changes in the anxiety-related NPY1 receptor and the reduced levels of BDNF, and its signalling receptor pTrkB, as well as the CREB protein. Brain DHA contents were significantly associated with the levels of anxiety-like behavior in these rats.

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Related in: MedlinePlus

Effect of diet switch to HFD on body weight gain and anxiety-like behaviors.(A) Diet switch to a HFD food significantly increased body weight gain as early as second week (p<0.0001) which remains significantly higher at the end of three weeks of HFD (p<0.0001) as compared to the rats on a healthy omega-3 supplemented diet. (B) Open field: significant decrease in the distance travelled in the open field (p<0.001) in rats switched to a HFD was noticed after 3 weeks of diet switch. (C–D) EPM: a non-significant trend toward decrease in percentage open arm entries in the rats subjected to diet switch to a HFD and a significant decrease in percentage time spent in open arm (p<0.05) in rats subjected to diet switch to a HFD was observed. Values are expressed in mean ±SEM. *p<0.05, *** p<0.001 Vs DHA diet.
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f2: Effect of diet switch to HFD on body weight gain and anxiety-like behaviors.(A) Diet switch to a HFD food significantly increased body weight gain as early as second week (p<0.0001) which remains significantly higher at the end of three weeks of HFD (p<0.0001) as compared to the rats on a healthy omega-3 supplemented diet. (B) Open field: significant decrease in the distance travelled in the open field (p<0.001) in rats switched to a HFD was noticed after 3 weeks of diet switch. (C–D) EPM: a non-significant trend toward decrease in percentage open arm entries in the rats subjected to diet switch to a HFD and a significant decrease in percentage time spent in open arm (p<0.05) in rats subjected to diet switch to a HFD was observed. Values are expressed in mean ±SEM. *p<0.05, *** p<0.001 Vs DHA diet.

Mentions: The diet transition to HFD in the animals previously on DHA diet (Fig. 1; experimental design) resulted in metabolic adverse effects. In the present study animals subjected to diet transition on a HFD for 3 weeks gain significantly more body weight as compared to their counterparts continued a healthy DHA supplemented diet (p< 0.001; Figure 2A). The animals fed HFD for 3 weeks also showed significantly higher blood glucose, cholesterol, triglycerides (p<0.05) and higher uric acid (p<0.01). The results are shown in Table 1.


High-fat diet transition reduces brain DHA levels associated with altered brain plasticity and behaviour.

Sharma S, Zhuang Y, Gomez-Pinilla F - Sci Rep (2012)

Effect of diet switch to HFD on body weight gain and anxiety-like behaviors.(A) Diet switch to a HFD food significantly increased body weight gain as early as second week (p<0.0001) which remains significantly higher at the end of three weeks of HFD (p<0.0001) as compared to the rats on a healthy omega-3 supplemented diet. (B) Open field: significant decrease in the distance travelled in the open field (p<0.001) in rats switched to a HFD was noticed after 3 weeks of diet switch. (C–D) EPM: a non-significant trend toward decrease in percentage open arm entries in the rats subjected to diet switch to a HFD and a significant decrease in percentage time spent in open arm (p<0.05) in rats subjected to diet switch to a HFD was observed. Values are expressed in mean ±SEM. *p<0.05, *** p<0.001 Vs DHA diet.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3362800&req=5

f2: Effect of diet switch to HFD on body weight gain and anxiety-like behaviors.(A) Diet switch to a HFD food significantly increased body weight gain as early as second week (p<0.0001) which remains significantly higher at the end of three weeks of HFD (p<0.0001) as compared to the rats on a healthy omega-3 supplemented diet. (B) Open field: significant decrease in the distance travelled in the open field (p<0.001) in rats switched to a HFD was noticed after 3 weeks of diet switch. (C–D) EPM: a non-significant trend toward decrease in percentage open arm entries in the rats subjected to diet switch to a HFD and a significant decrease in percentage time spent in open arm (p<0.05) in rats subjected to diet switch to a HFD was observed. Values are expressed in mean ±SEM. *p<0.05, *** p<0.001 Vs DHA diet.
Mentions: The diet transition to HFD in the animals previously on DHA diet (Fig. 1; experimental design) resulted in metabolic adverse effects. In the present study animals subjected to diet transition on a HFD for 3 weeks gain significantly more body weight as compared to their counterparts continued a healthy DHA supplemented diet (p< 0.001; Figure 2A). The animals fed HFD for 3 weeks also showed significantly higher blood glucose, cholesterol, triglycerides (p<0.05) and higher uric acid (p<0.01). The results are shown in Table 1.

Bottom Line: To assess how the shift from a healthy diet rich in omega-3 fatty acids to a diet rich in saturated fatty acid affects the substrates for brain plasticity and function, we used pregnant rats fed with omega-3 supplemented diet from their 2nd day of gestation period as well as their male pups for 12 weeks.We found that the HFD increased vulnerability for anxiety-like behavior, and that these modifications harmonized with changes in the anxiety-related NPY1 receptor and the reduced levels of BDNF, and its signalling receptor pTrkB, as well as the CREB protein.Brain DHA contents were significantly associated with the levels of anxiety-like behavior in these rats.

View Article: PubMed Central - PubMed

Affiliation: Dept. Integrative Biology & Physiology, UCLA, Los Angeles, CA 90095, USA.

ABSTRACT
To assess how the shift from a healthy diet rich in omega-3 fatty acids to a diet rich in saturated fatty acid affects the substrates for brain plasticity and function, we used pregnant rats fed with omega-3 supplemented diet from their 2nd day of gestation period as well as their male pups for 12 weeks. Afterwards, the animals were randomly assigned to either a group fed on the same diet or a group fed on a high-fat diet (HFD) rich in saturated fats for 3 weeks. We found that the HFD increased vulnerability for anxiety-like behavior, and that these modifications harmonized with changes in the anxiety-related NPY1 receptor and the reduced levels of BDNF, and its signalling receptor pTrkB, as well as the CREB protein. Brain DHA contents were significantly associated with the levels of anxiety-like behavior in these rats.

Show MeSH
Related in: MedlinePlus