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Introgression and characterization of a goatgrass gene for a high level of resistance to ug99 stem rust in tetraploid wheat.

Klindworth DL, Niu Z, Chao S, Friesen TL, Jin Y, Faris JD, Cai X, Xu SS - G3 (Bethesda) (2012)

Bottom Line: The second gene conditioned an IT 0; and was located on chromosome arm 2BL.Allosyndetic recombinant lines carrying each gene on minimal alien chromosomal segments were identified as were molecular markers distinguishing each alien segment.This study demonstrated that chromosome engineering of Ae. speltoides segments is feasible in tetraploid wheat.

View Article: PubMed Central - PubMed

ABSTRACT
The transfer of alien genes to crop plants using chromosome engineering has been attempted infrequently in tetraploid durum wheat (Triticum turgidum L. subsp. durum). Here, we report a highly efficient approach for the transfer of two genes conferring resistance to stem rust race Pgt-TTKSK (Ug99) from goatgrass (Aegilops speltoides) to tetraploid wheat. The durum line DAS15, carrying the stem rust resistance gene Sr47 derived from Ae. speltoides, was crossed, and backcrossed, to durum 5D(5B) aneuploids to induce homeologous pairing. After a final cross to 'Rusty' durum, allosyndetic recombinants were recovered. The Ae. speltoides chromosomal segment carrying Sr47 was found to have two stem rust resistance genes. One gene conditioning an infection type (IT) 2 was located in the same chromosomal region of 2BS as Sr39 and was assigned the temporary gene symbol SrAes7t. Based on ITs observed on a diverse set of rust races, SrAes7t may be the same as Sr39. The second gene conditioned an IT 0; and was located on chromosome arm 2BL. This gene retained the symbol Sr47 because it had a different IT and map location from other stem rust resistance genes derived from Ae. speltoides. Allosyndetic recombinant lines carrying each gene on minimal alien chromosomal segments were identified as were molecular markers distinguishing each alien segment. This study demonstrated that chromosome engineering of Ae. speltoides segments is feasible in tetraploid wheat. The Sr47 gene confers high-level and broad spectrum resistance to stem rust and should be very useful in efforts to control TTKSK.

No MeSH data available.


Related in: MedlinePlus

Stem rust ITs observed in (1) Rusty and (2−4) three BC2F1 plants of Rusty/3/Rusty 5D(5B)/DAS15//47-1 5D(5B). Plants 1 and 2 exhibit IT 34 (susceptible), whereas plant 3 has IT 2 and plant 4 has a IT fleck (0;).
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fig3: Stem rust ITs observed in (1) Rusty and (2−4) three BC2F1 plants of Rusty/3/Rusty 5D(5B)/DAS15//47-1 5D(5B). Plants 1 and 2 exhibit IT 34 (susceptible), whereas plant 3 has IT 2 and plant 4 has a IT fleck (0;).

Mentions: The 1086 BC2F1 plants were tested with TMLKC. There were 893 resistant and 193 susceptible plants, which did not fit a 1:1 segregation ratio (χ2 = 451.2, P < 0.001). Because Rusty was the female parent of the cross, the results indicated male gametes had strong segregation distortion favoring transmission of the Ae. speltoides segment. The resistant plants comprised two distinct ITs. There were 856 plants that had an IT 0; and 37 plants that had IT 2 (Figure 3), indicating that the Ae. speltoides segment in DAS15 carried two stem rust resistance genes. The two genes are here temporarily referred to as the IT 0; and IT 2 gene. The 1086 BC2F1 plants were genotyped with the five SSR markers, Xgwm55 (Figure S3), Xgwm319, Xwmc474, Xbarc55, and Xcfa2278, using capillary electrophoresis. The marker analysis and stem rust test identified 81 allosyndetic recombinant plants (Table S2) that are summarized in Table 1. Forty-two of the plants had IT 0; and 37 plants had IT 2. Among the IT 2 plants, 32 retained the Ae. speltoides alleles for all five SSR markers. These 32 plants were identified as new allosyndetic recombinants because of the absence of the IT 0; gene. Two of the 81 allosyndetic recombinant plants were susceptible to TMLKC (Table 1 and Table S2). For both plants, the wheat alleles at four of the five SSR loci were replaced by the Ae. speltoides alleles.


Introgression and characterization of a goatgrass gene for a high level of resistance to ug99 stem rust in tetraploid wheat.

Klindworth DL, Niu Z, Chao S, Friesen TL, Jin Y, Faris JD, Cai X, Xu SS - G3 (Bethesda) (2012)

Stem rust ITs observed in (1) Rusty and (2−4) three BC2F1 plants of Rusty/3/Rusty 5D(5B)/DAS15//47-1 5D(5B). Plants 1 and 2 exhibit IT 34 (susceptible), whereas plant 3 has IT 2 and plant 4 has a IT fleck (0;).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3362296&req=5

fig3: Stem rust ITs observed in (1) Rusty and (2−4) three BC2F1 plants of Rusty/3/Rusty 5D(5B)/DAS15//47-1 5D(5B). Plants 1 and 2 exhibit IT 34 (susceptible), whereas plant 3 has IT 2 and plant 4 has a IT fleck (0;).
Mentions: The 1086 BC2F1 plants were tested with TMLKC. There were 893 resistant and 193 susceptible plants, which did not fit a 1:1 segregation ratio (χ2 = 451.2, P < 0.001). Because Rusty was the female parent of the cross, the results indicated male gametes had strong segregation distortion favoring transmission of the Ae. speltoides segment. The resistant plants comprised two distinct ITs. There were 856 plants that had an IT 0; and 37 plants that had IT 2 (Figure 3), indicating that the Ae. speltoides segment in DAS15 carried two stem rust resistance genes. The two genes are here temporarily referred to as the IT 0; and IT 2 gene. The 1086 BC2F1 plants were genotyped with the five SSR markers, Xgwm55 (Figure S3), Xgwm319, Xwmc474, Xbarc55, and Xcfa2278, using capillary electrophoresis. The marker analysis and stem rust test identified 81 allosyndetic recombinant plants (Table S2) that are summarized in Table 1. Forty-two of the plants had IT 0; and 37 plants had IT 2. Among the IT 2 plants, 32 retained the Ae. speltoides alleles for all five SSR markers. These 32 plants were identified as new allosyndetic recombinants because of the absence of the IT 0; gene. Two of the 81 allosyndetic recombinant plants were susceptible to TMLKC (Table 1 and Table S2). For both plants, the wheat alleles at four of the five SSR loci were replaced by the Ae. speltoides alleles.

Bottom Line: The second gene conditioned an IT 0; and was located on chromosome arm 2BL.Allosyndetic recombinant lines carrying each gene on minimal alien chromosomal segments were identified as were molecular markers distinguishing each alien segment.This study demonstrated that chromosome engineering of Ae. speltoides segments is feasible in tetraploid wheat.

View Article: PubMed Central - PubMed

ABSTRACT
The transfer of alien genes to crop plants using chromosome engineering has been attempted infrequently in tetraploid durum wheat (Triticum turgidum L. subsp. durum). Here, we report a highly efficient approach for the transfer of two genes conferring resistance to stem rust race Pgt-TTKSK (Ug99) from goatgrass (Aegilops speltoides) to tetraploid wheat. The durum line DAS15, carrying the stem rust resistance gene Sr47 derived from Ae. speltoides, was crossed, and backcrossed, to durum 5D(5B) aneuploids to induce homeologous pairing. After a final cross to 'Rusty' durum, allosyndetic recombinants were recovered. The Ae. speltoides chromosomal segment carrying Sr47 was found to have two stem rust resistance genes. One gene conditioning an infection type (IT) 2 was located in the same chromosomal region of 2BS as Sr39 and was assigned the temporary gene symbol SrAes7t. Based on ITs observed on a diverse set of rust races, SrAes7t may be the same as Sr39. The second gene conditioned an IT 0; and was located on chromosome arm 2BL. This gene retained the symbol Sr47 because it had a different IT and map location from other stem rust resistance genes derived from Ae. speltoides. Allosyndetic recombinant lines carrying each gene on minimal alien chromosomal segments were identified as were molecular markers distinguishing each alien segment. This study demonstrated that chromosome engineering of Ae. speltoides segments is feasible in tetraploid wheat. The Sr47 gene confers high-level and broad spectrum resistance to stem rust and should be very useful in efforts to control TTKSK.

No MeSH data available.


Related in: MedlinePlus