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Expression of neutral endopeptidase, endothelin-1, and nuclear factor kappa B in prostate cancer: interrelations and associations with prostate-specific antigen recurrence after radical prostatectomy.

Vlachostergios PJ, Karasavvidou F, Kakkas G, Moutzouris G, Patrikidou A, Voutsadakis IA, Daliani DD, Zintzaras E, Melekos MD, Papandreou CN - Prostate Cancer (2012)

Bottom Line: NEP was associated with ET-1 (P < 0.001) and NFκB (P < 0.001).Conclusions.There seems to be a clinical model of NEP/NPs and NFκB pathways interconnection, with their constituents following inverse patterns of expression in accordance with their biological roles and molecular interrelations.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Oncology, University Hospital of Larissa, University of Thessaly School of Medicine, 41110 Larissa, Greece.

ABSTRACT
Objective. To study the impact of the neutral endopeptidase (NEP)/neuropeptides (NPs) axis and nuclear factor kappa B (NFκB) as predictors of prostate-specific antigen (PSA) recurrence after radical prostatectomy (RP). Patients and Methods. 70 patients with early-stage PC were treated with RP and their tumor samples were evaluated for expression of NEP, endothelin-1 (ET-1) and NFκB (p65). Time to PSA recurrence was correlated with the examined parameters and combined with preoperative PSA level, Gleason score, pathological TNM (pT) stage, and surgical margin (SM) assessment. Results and Limitations. Membranous expression of NEP (P < 0.001), cytoplasmic ET-1 (P = 0.002), and cytoplasmic NFκB (P < 0.001) were correlated with time to PSA relapse. NEP was associated with ET-1 (P < 0.001) and NFκB (P < 0.001). ET-1 was also correlated with NFκB (P < 0.001). NEP expression (P = 0.017), pT stage (P = 0.013), and SMs (P = 0.036) were independent predictors of time to PSA recurrence. Conclusions. There seems to be a clinical model of NEP/NPs and NFκB pathways interconnection, with their constituents following inverse patterns of expression in accordance with their biological roles and molecular interrelations.

No MeSH data available.


Related in: MedlinePlus

Prostate adenocarcinoma Gleason pattern 3. The same area as in Figure 4. (a) Weak-to-moderate cytoplasmic IHC staining for NFκB (×400); (b) Negative immunoreactivity for ET-1. Positive marker the capillary endothelium (arrows) (×200).
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fig5: Prostate adenocarcinoma Gleason pattern 3. The same area as in Figure 4. (a) Weak-to-moderate cytoplasmic IHC staining for NFκB (×400); (b) Negative immunoreactivity for ET-1. Positive marker the capillary endothelium (arrows) (×200).

Mentions: In univariate analysis, a significant correlation was found between IHC expression of NEP and ET-1 (P < 0.001). Same was the case for the NEP-NFκB relationship (P < 0.001) (Table 3). ET-1 and NFκB expressions were also interrelated (P < 0.001). IHC expression patterns of NEP, ET-1, and NFκB are depicted in Figures 4, 5 and 6.


Expression of neutral endopeptidase, endothelin-1, and nuclear factor kappa B in prostate cancer: interrelations and associations with prostate-specific antigen recurrence after radical prostatectomy.

Vlachostergios PJ, Karasavvidou F, Kakkas G, Moutzouris G, Patrikidou A, Voutsadakis IA, Daliani DD, Zintzaras E, Melekos MD, Papandreou CN - Prostate Cancer (2012)

Prostate adenocarcinoma Gleason pattern 3. The same area as in Figure 4. (a) Weak-to-moderate cytoplasmic IHC staining for NFκB (×400); (b) Negative immunoreactivity for ET-1. Positive marker the capillary endothelium (arrows) (×200).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3362215&req=5

fig5: Prostate adenocarcinoma Gleason pattern 3. The same area as in Figure 4. (a) Weak-to-moderate cytoplasmic IHC staining for NFκB (×400); (b) Negative immunoreactivity for ET-1. Positive marker the capillary endothelium (arrows) (×200).
Mentions: In univariate analysis, a significant correlation was found between IHC expression of NEP and ET-1 (P < 0.001). Same was the case for the NEP-NFκB relationship (P < 0.001) (Table 3). ET-1 and NFκB expressions were also interrelated (P < 0.001). IHC expression patterns of NEP, ET-1, and NFκB are depicted in Figures 4, 5 and 6.

Bottom Line: NEP was associated with ET-1 (P < 0.001) and NFκB (P < 0.001).Conclusions.There seems to be a clinical model of NEP/NPs and NFκB pathways interconnection, with their constituents following inverse patterns of expression in accordance with their biological roles and molecular interrelations.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Oncology, University Hospital of Larissa, University of Thessaly School of Medicine, 41110 Larissa, Greece.

ABSTRACT
Objective. To study the impact of the neutral endopeptidase (NEP)/neuropeptides (NPs) axis and nuclear factor kappa B (NFκB) as predictors of prostate-specific antigen (PSA) recurrence after radical prostatectomy (RP). Patients and Methods. 70 patients with early-stage PC were treated with RP and their tumor samples were evaluated for expression of NEP, endothelin-1 (ET-1) and NFκB (p65). Time to PSA recurrence was correlated with the examined parameters and combined with preoperative PSA level, Gleason score, pathological TNM (pT) stage, and surgical margin (SM) assessment. Results and Limitations. Membranous expression of NEP (P < 0.001), cytoplasmic ET-1 (P = 0.002), and cytoplasmic NFκB (P < 0.001) were correlated with time to PSA relapse. NEP was associated with ET-1 (P < 0.001) and NFκB (P < 0.001). ET-1 was also correlated with NFκB (P < 0.001). NEP expression (P = 0.017), pT stage (P = 0.013), and SMs (P = 0.036) were independent predictors of time to PSA recurrence. Conclusions. There seems to be a clinical model of NEP/NPs and NFκB pathways interconnection, with their constituents following inverse patterns of expression in accordance with their biological roles and molecular interrelations.

No MeSH data available.


Related in: MedlinePlus