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Human mature adipocytes express albumin and this expression is not regulated by inflammation.

Sirico ML, Guida B, Procino A, Pota A, Sodo M, Grandaliano G, Simone S, Pertosa G, Riccio E, Memoli B - Mediators Inflamm. (2012)

Bottom Line: Albumin gene expression resulted significantly higher in pre-adipocytes than in adipocytes.In the present study we found first time evidence that human adipocytes express albumin.Our results also showed that systemic inflammation does not modulate albumin gene expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, University Federico II of Naples, 80131 Naples, Italy. ml_sirico@yahoo.it

ABSTRACT

Aims: Our group investigated albumin gene expression in human adipocytes, its regulation by inflammation and the possible contribution of adipose tissue to albumin circulating levels.

Methods: Both inflamed and healthy subjects provided adipose tissue samples. RT-PCR, Real-Time PCR, and Western Blot analysis on homogenates of adipocytes and pre-adipocytes were performed. In sixty-three healthy subjects and fifty-four micro-inflamed end stage renal disease (ESRD) patients circulating levels of albumin were measured by nephelometry; all subjects were also evaluated for body composition, calculated from bioelectrical measurements and an thropometric data.

Results: A clear gene expression of albumin was showed in pre-adipocytes and, for the first time, in mature adipocytes. Albumin gene expression resulted significantly higher in pre-adipocytes than in adipocytes. No significant difference in albumin gene expression was showed between healthy controls and inflamed patients. A significant negative correlation was observed between albumin levels and fat mass in both healthy subjects and inflamed ESRD patients.

Conclusions: In the present study we found first time evidence that human adipocytes express albumin. Our results also showed that systemic inflammation does not modulate albumin gene expression. The negative correlation between albumin and fat mass seems to exclude a significant contributing role of adipocyte in plasma albumin.

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Related in: MedlinePlus

Albumin gene expression in the adipocytes and pre-adipocytes from different sites of adipose tissue of a single noninflamed subject, as representative of six experiments for all the subjects enrolled, obtained by real-time PCR analysis. The columns show the relative expression of albumin mRNA in Sc (gray bars) and Om (black bars) adipocyte (Sc-AD and Om-AD) and preadipocyte (PreAD) of a single healthy subject, as representative. The values on the y-axis represent arbitrary units derived from the mean expression value for albumin gene.
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fig3: Albumin gene expression in the adipocytes and pre-adipocytes from different sites of adipose tissue of a single noninflamed subject, as representative of six experiments for all the subjects enrolled, obtained by real-time PCR analysis. The columns show the relative expression of albumin mRNA in Sc (gray bars) and Om (black bars) adipocyte (Sc-AD and Om-AD) and preadipocyte (PreAD) of a single healthy subject, as representative. The values on the y-axis represent arbitrary units derived from the mean expression value for albumin gene.

Mentions: We did not find any statistically significant difference in albumin gene expression between inflamed and noninflamed patients in adipocytes drawn from both Sc and Om adipose tissue. As Figure 2 shows, we only observed an higher omental adipocyte albumin than subcutaneous one in each group. We concluded that inflammation does not modulate albumin gene expression in the adipocyte, but probably further studies are required to confirm this result. Figure 3 shows the different albumin gene expression, studied by real-time PCR, in adipocytes and pre-adipocytes obtained from either Sc or Om fragments of adipose tissue drawn from noninflamed subjects. Pre-adipocyte albumin gene expression was higher then the adipocyte one in both Om and Sc fragments and this difference was statistically significant. We also observed that albumin gene expression in the pre-adipocyte extracted from fragments of Om adipose tissue was significantly higher than in preadipocyte from Sc fragments. No difference was found between the adipocytes from different districts (Figure 3).


Human mature adipocytes express albumin and this expression is not regulated by inflammation.

Sirico ML, Guida B, Procino A, Pota A, Sodo M, Grandaliano G, Simone S, Pertosa G, Riccio E, Memoli B - Mediators Inflamm. (2012)

Albumin gene expression in the adipocytes and pre-adipocytes from different sites of adipose tissue of a single noninflamed subject, as representative of six experiments for all the subjects enrolled, obtained by real-time PCR analysis. The columns show the relative expression of albumin mRNA in Sc (gray bars) and Om (black bars) adipocyte (Sc-AD and Om-AD) and preadipocyte (PreAD) of a single healthy subject, as representative. The values on the y-axis represent arbitrary units derived from the mean expression value for albumin gene.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3362193&req=5

fig3: Albumin gene expression in the adipocytes and pre-adipocytes from different sites of adipose tissue of a single noninflamed subject, as representative of six experiments for all the subjects enrolled, obtained by real-time PCR analysis. The columns show the relative expression of albumin mRNA in Sc (gray bars) and Om (black bars) adipocyte (Sc-AD and Om-AD) and preadipocyte (PreAD) of a single healthy subject, as representative. The values on the y-axis represent arbitrary units derived from the mean expression value for albumin gene.
Mentions: We did not find any statistically significant difference in albumin gene expression between inflamed and noninflamed patients in adipocytes drawn from both Sc and Om adipose tissue. As Figure 2 shows, we only observed an higher omental adipocyte albumin than subcutaneous one in each group. We concluded that inflammation does not modulate albumin gene expression in the adipocyte, but probably further studies are required to confirm this result. Figure 3 shows the different albumin gene expression, studied by real-time PCR, in adipocytes and pre-adipocytes obtained from either Sc or Om fragments of adipose tissue drawn from noninflamed subjects. Pre-adipocyte albumin gene expression was higher then the adipocyte one in both Om and Sc fragments and this difference was statistically significant. We also observed that albumin gene expression in the pre-adipocyte extracted from fragments of Om adipose tissue was significantly higher than in preadipocyte from Sc fragments. No difference was found between the adipocytes from different districts (Figure 3).

Bottom Line: Albumin gene expression resulted significantly higher in pre-adipocytes than in adipocytes.In the present study we found first time evidence that human adipocytes express albumin.Our results also showed that systemic inflammation does not modulate albumin gene expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, University Federico II of Naples, 80131 Naples, Italy. ml_sirico@yahoo.it

ABSTRACT

Aims: Our group investigated albumin gene expression in human adipocytes, its regulation by inflammation and the possible contribution of adipose tissue to albumin circulating levels.

Methods: Both inflamed and healthy subjects provided adipose tissue samples. RT-PCR, Real-Time PCR, and Western Blot analysis on homogenates of adipocytes and pre-adipocytes were performed. In sixty-three healthy subjects and fifty-four micro-inflamed end stage renal disease (ESRD) patients circulating levels of albumin were measured by nephelometry; all subjects were also evaluated for body composition, calculated from bioelectrical measurements and an thropometric data.

Results: A clear gene expression of albumin was showed in pre-adipocytes and, for the first time, in mature adipocytes. Albumin gene expression resulted significantly higher in pre-adipocytes than in adipocytes. No significant difference in albumin gene expression was showed between healthy controls and inflamed patients. A significant negative correlation was observed between albumin levels and fat mass in both healthy subjects and inflamed ESRD patients.

Conclusions: In the present study we found first time evidence that human adipocytes express albumin. Our results also showed that systemic inflammation does not modulate albumin gene expression. The negative correlation between albumin and fat mass seems to exclude a significant contributing role of adipocyte in plasma albumin.

Show MeSH
Related in: MedlinePlus