Limits...
The Association of IL-12b Polymorphisms with Systemic Lupus Erythematosus in Chinese Han Population.

Shao Y, Zhang J, Chen Y, Wu Q, Guan M, Yu B, Wan J, Zhang W - Int J Rheumatol (2012)

Bottom Line: Minor allele (C) of rs6887695 (P = 0.031, OR 0.78, [95% CI 0.63-0.98]) was found to be protective against SLE.Minor allele (C) exerts protective effect on the incidence of arthritis (P = 0.013, OR = 0.65, 95% CI = 0.47-0.92) and abnormalities of antinuclear antibody (P = 0.022, OR = 0.68, 95% CI = 0.49-0.95).Summary.

View Article: PubMed Central - PubMed

Affiliation: Shenzhen Key Lab for Translational Medicine of Dermatology, Shenzhen PKU-HKUST Medical Center, No. 1120, Lianhua Road, Futian District, Shenzhen, Guangdong 518036, China.

ABSTRACT
Background. Systemic lupus erythematosus (SLE) is a complex immune disease. The genetic variation in the IL-12b gene was found to associate with SLE in Caucasian population. In this study, we examined this association in Chinese Han population by a recently developed method, unlabeled probe-based high resolution melting analysis. Methods. A total of 297 SLE patients and 351 controls were recruited. Unlabeled probe-based high resolution melting analysis (HRMA) was used in genotyping. Results. Statistically significant differences were observed in both genotype and allele frequencies for rs6887695 in the SLE patients as compared with the controls. Minor allele (C) of rs6887695 (P = 0.031, OR 0.78, [95% CI 0.63-0.98]) was found to be protective against SLE. The association of SNP rs6887695 with the diagnostic criteria of SLE was also examined. Minor allele (C) exerts protective effect on the incidence of arthritis (P = 0.013, OR = 0.65, 95% CI = 0.47-0.92) and abnormalities of antinuclear antibody (P = 0.022, OR = 0.68, 95% CI = 0.49-0.95). IL-12b SNPs were irrelevant to other diagnostic criteria of SLE. Summary. Polymorphisms of rs6887695 in IL-12b gene were associated with disease risk, as well as arthritis and antinuclear antibody synthesis, of systemic lupus erythematosus in Chinese population.

No MeSH data available.


Related in: MedlinePlus

Derivative melting curves of unlabeled probes and amplicon for genotyping of SNP rs6887695. Three genotypes (GG, GC and CC) were discriminated as indicated in probe region.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3362034&req=5

fig1: Derivative melting curves of unlabeled probes and amplicon for genotyping of SNP rs6887695. Three genotypes (GG, GC and CC) were discriminated as indicated in probe region.

Mentions: High-resolution melting analysis with unlabeled C3-blocked probe was used for genotyping. As shown in Figure 1, three genotypes (GG, GC, and CC) of SNP rs6887695 (G > C) were accurately distinguished by the derivative melting curves in the probe region. The derivative melting curves directly from the PCR products were not able to distinguish these genotypes (Figure 1, right part), and the sensitivity and accuracy of HRMA were dramatically improved using unlabeled probes (Figure 1, left part). After further analyzing on the normalized melting unlabeled probe region, we were able to discriminate all the genotypes clearly (Figure 2(a)). The difference curves generated by subtracting of one heterozygote curve displayed a much more clear view for genotype discrimination (Figure 2(b)). Then, this method was employed to screen our samples in the present study.


The Association of IL-12b Polymorphisms with Systemic Lupus Erythematosus in Chinese Han Population.

Shao Y, Zhang J, Chen Y, Wu Q, Guan M, Yu B, Wan J, Zhang W - Int J Rheumatol (2012)

Derivative melting curves of unlabeled probes and amplicon for genotyping of SNP rs6887695. Three genotypes (GG, GC and CC) were discriminated as indicated in probe region.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3362034&req=5

fig1: Derivative melting curves of unlabeled probes and amplicon for genotyping of SNP rs6887695. Three genotypes (GG, GC and CC) were discriminated as indicated in probe region.
Mentions: High-resolution melting analysis with unlabeled C3-blocked probe was used for genotyping. As shown in Figure 1, three genotypes (GG, GC, and CC) of SNP rs6887695 (G > C) were accurately distinguished by the derivative melting curves in the probe region. The derivative melting curves directly from the PCR products were not able to distinguish these genotypes (Figure 1, right part), and the sensitivity and accuracy of HRMA were dramatically improved using unlabeled probes (Figure 1, left part). After further analyzing on the normalized melting unlabeled probe region, we were able to discriminate all the genotypes clearly (Figure 2(a)). The difference curves generated by subtracting of one heterozygote curve displayed a much more clear view for genotype discrimination (Figure 2(b)). Then, this method was employed to screen our samples in the present study.

Bottom Line: Minor allele (C) of rs6887695 (P = 0.031, OR 0.78, [95% CI 0.63-0.98]) was found to be protective against SLE.Minor allele (C) exerts protective effect on the incidence of arthritis (P = 0.013, OR = 0.65, 95% CI = 0.47-0.92) and abnormalities of antinuclear antibody (P = 0.022, OR = 0.68, 95% CI = 0.49-0.95).Summary.

View Article: PubMed Central - PubMed

Affiliation: Shenzhen Key Lab for Translational Medicine of Dermatology, Shenzhen PKU-HKUST Medical Center, No. 1120, Lianhua Road, Futian District, Shenzhen, Guangdong 518036, China.

ABSTRACT
Background. Systemic lupus erythematosus (SLE) is a complex immune disease. The genetic variation in the IL-12b gene was found to associate with SLE in Caucasian population. In this study, we examined this association in Chinese Han population by a recently developed method, unlabeled probe-based high resolution melting analysis. Methods. A total of 297 SLE patients and 351 controls were recruited. Unlabeled probe-based high resolution melting analysis (HRMA) was used in genotyping. Results. Statistically significant differences were observed in both genotype and allele frequencies for rs6887695 in the SLE patients as compared with the controls. Minor allele (C) of rs6887695 (P = 0.031, OR 0.78, [95% CI 0.63-0.98]) was found to be protective against SLE. The association of SNP rs6887695 with the diagnostic criteria of SLE was also examined. Minor allele (C) exerts protective effect on the incidence of arthritis (P = 0.013, OR = 0.65, 95% CI = 0.47-0.92) and abnormalities of antinuclear antibody (P = 0.022, OR = 0.68, 95% CI = 0.49-0.95). IL-12b SNPs were irrelevant to other diagnostic criteria of SLE. Summary. Polymorphisms of rs6887695 in IL-12b gene were associated with disease risk, as well as arthritis and antinuclear antibody synthesis, of systemic lupus erythematosus in Chinese population.

No MeSH data available.


Related in: MedlinePlus