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Imunocompetent mice model for dengue virus infection.

Gonçalves D, de Queiroz Prado R, Almeida Xavier E, Cristina de Oliveira N, da Matta Guedes PM, da Silva JS, Moraes Figueiredo LT, Aquino VH - ScientificWorldJournal (2012)

Bottom Line: DENV belongs to the family Flaviviridae, genus Flavivirus, and is classified into four antigenically distinct serotypes: DENV-1, DENV-2, DENV-3, and DENV-4.The number of nations and people affected has increased steadily and today is considered the most widely spread arbovirus (arthropod-borne viral disease) in the world.Moreover, the animals became viremic and the virus was detected in several organs by real-time RT-PCR.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Análises Clínicas, Toxicológicas e Bromatológicas da Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Monte Alegre, 14049-900 Ribeirao Preto, SP, Brazil. g_denise87@yahoo.com.br

ABSTRACT
Dengue fever is a noncontagious infectious disease caused by dengue virus (DENV). DENV belongs to the family Flaviviridae, genus Flavivirus, and is classified into four antigenically distinct serotypes: DENV-1, DENV-2, DENV-3, and DENV-4. The number of nations and people affected has increased steadily and today is considered the most widely spread arbovirus (arthropod-borne viral disease) in the world. The absence of an appropriate animal model for studying the disease has hindered the understanding of dengue pathogenesis. In our study, we have found that immunocompetent C57BL/6 mice infected intraperitoneally with DENV-1 presented some signs of dengue disease such as thrombocytopenia, spleen hemorrhage, liver damage, and increase in production of IFNγ and TNFα cytokines. Moreover, the animals became viremic and the virus was detected in several organs by real-time RT-PCR. Thus, this animal model could be used to study mechanism of dengue virus infection, to test antiviral drugs, as well as to evaluate candidate vaccines.

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Percentage of CD4+ (a), CD8+ (b), dendritic cells (c), macrophages (d), and regulatory T cells ((e) and (f)) from the spleen in the third, sixth, and ninth days of infection with DENV1. The data were analyzed using the Student's t-test and differences were considered significant when P < 0.05 (*).
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fig11: Percentage of CD4+ (a), CD8+ (b), dendritic cells (c), macrophages (d), and regulatory T cells ((e) and (f)) from the spleen in the third, sixth, and ninth days of infection with DENV1. The data were analyzed using the Student's t-test and differences were considered significant when P < 0.05 (*).

Mentions: The phenotype of immune cells in the spleen was investigated by flow cytometry analysis on days 3, 6, and 9 after infection (Figure 11). No significant increase of the number of CD4+ and CD8+ T lymphocytes and dendritic cells was observed in the animals in any day after infection when compared to the uninfected animal. However, an increase in the population of macrophage (CD11c− CD11b+ phenotype) cells was observed in the infected animals three days after infection. Were also determined the percentage of regulatory T cells CD4+ CD25hi which showed similar levels between infected and uninfected mice, but this population of cells was an apparent decrease in the expression of CTLA4 on the third day of infection.


Imunocompetent mice model for dengue virus infection.

Gonçalves D, de Queiroz Prado R, Almeida Xavier E, Cristina de Oliveira N, da Matta Guedes PM, da Silva JS, Moraes Figueiredo LT, Aquino VH - ScientificWorldJournal (2012)

Percentage of CD4+ (a), CD8+ (b), dendritic cells (c), macrophages (d), and regulatory T cells ((e) and (f)) from the spleen in the third, sixth, and ninth days of infection with DENV1. The data were analyzed using the Student's t-test and differences were considered significant when P < 0.05 (*).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3362018&req=5

fig11: Percentage of CD4+ (a), CD8+ (b), dendritic cells (c), macrophages (d), and regulatory T cells ((e) and (f)) from the spleen in the third, sixth, and ninth days of infection with DENV1. The data were analyzed using the Student's t-test and differences were considered significant when P < 0.05 (*).
Mentions: The phenotype of immune cells in the spleen was investigated by flow cytometry analysis on days 3, 6, and 9 after infection (Figure 11). No significant increase of the number of CD4+ and CD8+ T lymphocytes and dendritic cells was observed in the animals in any day after infection when compared to the uninfected animal. However, an increase in the population of macrophage (CD11c− CD11b+ phenotype) cells was observed in the infected animals three days after infection. Were also determined the percentage of regulatory T cells CD4+ CD25hi which showed similar levels between infected and uninfected mice, but this population of cells was an apparent decrease in the expression of CTLA4 on the third day of infection.

Bottom Line: DENV belongs to the family Flaviviridae, genus Flavivirus, and is classified into four antigenically distinct serotypes: DENV-1, DENV-2, DENV-3, and DENV-4.The number of nations and people affected has increased steadily and today is considered the most widely spread arbovirus (arthropod-borne viral disease) in the world.Moreover, the animals became viremic and the virus was detected in several organs by real-time RT-PCR.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Análises Clínicas, Toxicológicas e Bromatológicas da Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Monte Alegre, 14049-900 Ribeirao Preto, SP, Brazil. g_denise87@yahoo.com.br

ABSTRACT
Dengue fever is a noncontagious infectious disease caused by dengue virus (DENV). DENV belongs to the family Flaviviridae, genus Flavivirus, and is classified into four antigenically distinct serotypes: DENV-1, DENV-2, DENV-3, and DENV-4. The number of nations and people affected has increased steadily and today is considered the most widely spread arbovirus (arthropod-borne viral disease) in the world. The absence of an appropriate animal model for studying the disease has hindered the understanding of dengue pathogenesis. In our study, we have found that immunocompetent C57BL/6 mice infected intraperitoneally with DENV-1 presented some signs of dengue disease such as thrombocytopenia, spleen hemorrhage, liver damage, and increase in production of IFNγ and TNFα cytokines. Moreover, the animals became viremic and the virus was detected in several organs by real-time RT-PCR. Thus, this animal model could be used to study mechanism of dengue virus infection, to test antiviral drugs, as well as to evaluate candidate vaccines.

Show MeSH
Related in: MedlinePlus