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Imunocompetent mice model for dengue virus infection.

Gonçalves D, de Queiroz Prado R, Almeida Xavier E, Cristina de Oliveira N, da Matta Guedes PM, da Silva JS, Moraes Figueiredo LT, Aquino VH - ScientificWorldJournal (2012)

Bottom Line: DENV belongs to the family Flaviviridae, genus Flavivirus, and is classified into four antigenically distinct serotypes: DENV-1, DENV-2, DENV-3, and DENV-4.The number of nations and people affected has increased steadily and today is considered the most widely spread arbovirus (arthropod-borne viral disease) in the world.Moreover, the animals became viremic and the virus was detected in several organs by real-time RT-PCR.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Análises Clínicas, Toxicológicas e Bromatológicas da Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Monte Alegre, 14049-900 Ribeirao Preto, SP, Brazil. g_denise87@yahoo.com.br

ABSTRACT
Dengue fever is a noncontagious infectious disease caused by dengue virus (DENV). DENV belongs to the family Flaviviridae, genus Flavivirus, and is classified into four antigenically distinct serotypes: DENV-1, DENV-2, DENV-3, and DENV-4. The number of nations and people affected has increased steadily and today is considered the most widely spread arbovirus (arthropod-borne viral disease) in the world. The absence of an appropriate animal model for studying the disease has hindered the understanding of dengue pathogenesis. In our study, we have found that immunocompetent C57BL/6 mice infected intraperitoneally with DENV-1 presented some signs of dengue disease such as thrombocytopenia, spleen hemorrhage, liver damage, and increase in production of IFNγ and TNFα cytokines. Moreover, the animals became viremic and the virus was detected in several organs by real-time RT-PCR. Thus, this animal model could be used to study mechanism of dengue virus infection, to test antiviral drugs, as well as to evaluate candidate vaccines.

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Histology of liver of uninfected mice (a); seven days after infection (b) and 10 days after infection (c). The arrows indicate the presence of cellular infiltrate. Eosin and Hematoxylin staining. The magnification was 200x.
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fig6: Histology of liver of uninfected mice (a); seven days after infection (b) and 10 days after infection (c). The arrows indicate the presence of cellular infiltrate. Eosin and Hematoxylin staining. The magnification was 200x.

Mentions: To further investigate the presence of cellular infiltrate in the liver, the animals were infected and 0, 2, 4, 7, 10, 16, and 21 after infection a histological analysis of the liver was carried out. The analysis of the slides showed the presence of cellular infiltrate in the liver, with a significant increase ten days after infection (Figures 6 and 7).


Imunocompetent mice model for dengue virus infection.

Gonçalves D, de Queiroz Prado R, Almeida Xavier E, Cristina de Oliveira N, da Matta Guedes PM, da Silva JS, Moraes Figueiredo LT, Aquino VH - ScientificWorldJournal (2012)

Histology of liver of uninfected mice (a); seven days after infection (b) and 10 days after infection (c). The arrows indicate the presence of cellular infiltrate. Eosin and Hematoxylin staining. The magnification was 200x.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3362018&req=5

fig6: Histology of liver of uninfected mice (a); seven days after infection (b) and 10 days after infection (c). The arrows indicate the presence of cellular infiltrate. Eosin and Hematoxylin staining. The magnification was 200x.
Mentions: To further investigate the presence of cellular infiltrate in the liver, the animals were infected and 0, 2, 4, 7, 10, 16, and 21 after infection a histological analysis of the liver was carried out. The analysis of the slides showed the presence of cellular infiltrate in the liver, with a significant increase ten days after infection (Figures 6 and 7).

Bottom Line: DENV belongs to the family Flaviviridae, genus Flavivirus, and is classified into four antigenically distinct serotypes: DENV-1, DENV-2, DENV-3, and DENV-4.The number of nations and people affected has increased steadily and today is considered the most widely spread arbovirus (arthropod-borne viral disease) in the world.Moreover, the animals became viremic and the virus was detected in several organs by real-time RT-PCR.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Análises Clínicas, Toxicológicas e Bromatológicas da Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Monte Alegre, 14049-900 Ribeirao Preto, SP, Brazil. g_denise87@yahoo.com.br

ABSTRACT
Dengue fever is a noncontagious infectious disease caused by dengue virus (DENV). DENV belongs to the family Flaviviridae, genus Flavivirus, and is classified into four antigenically distinct serotypes: DENV-1, DENV-2, DENV-3, and DENV-4. The number of nations and people affected has increased steadily and today is considered the most widely spread arbovirus (arthropod-borne viral disease) in the world. The absence of an appropriate animal model for studying the disease has hindered the understanding of dengue pathogenesis. In our study, we have found that immunocompetent C57BL/6 mice infected intraperitoneally with DENV-1 presented some signs of dengue disease such as thrombocytopenia, spleen hemorrhage, liver damage, and increase in production of IFNγ and TNFα cytokines. Moreover, the animals became viremic and the virus was detected in several organs by real-time RT-PCR. Thus, this animal model could be used to study mechanism of dengue virus infection, to test antiviral drugs, as well as to evaluate candidate vaccines.

Show MeSH
Related in: MedlinePlus