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Serum heparan sulfate levels are elevated in endotoxemia.

Hofmann-Kiefer KF, Kemming GI, Chappell D, Flondor M, Kisch-Wedel H, Hauser A, Pallivathukal S, Conzen P, Rehm M - Eur. J. Med. Res. (2009)

Bottom Line: Serum heparan sulfate concentrations significantly increased over time in the endotoxin group and were significantly elevated in comparison to the control group 6 hours after administration of endotoxin (p<0.001).In the endotoxin group all markers of inflammation significantly changed during the time course.The administration of bacterial endotoxin induced a significant rise in degradation products of the endothelial glycocalyx.

View Article: PubMed Central - HTML - PubMed

Affiliation: Clinic of Anesthesiology/Critical Care Medicine and Pain Therapy (M.A.B., P.C), Ludwig-Maximilians-University, City of Munich, Germany. Klaus.Hofmann-Kiefer@med.uni-muenchen.de

ABSTRACT

Background: Increased vascular permeability is a characteristic feature of sepsis which, in the past, has been ascribed exclusively to a malfunction of endothelial cells. However, recently it has become evident that the endothelial glycocalyx is of considerable importance concerning various aspects of vascular physiology, e.g. the vascular barrier and inflammation. Heparan sulfate, one of its essential components is characteristically traceable in blood, in case the endothelial glycocalyx is damaged or destroyed.

Methods: In 15 pigs we investigated whether the administration of endotoxin from gram-negative bacteria (Escherichia coli) results in increased serum levels of heparan sulfate, signalizing a shedding of the glycocalyx. In addition, markers of inflammation (white blood cell count, platelet count, tumour necrosis factor-α and interleukin-6) were evaluated over an observation period of 6 hours.

Results: Serum heparan sulfate concentrations significantly increased over time in the endotoxin group and were significantly elevated in comparison to the control group 6 hours after administration of endotoxin (p<0.001). In the endotoxin group all markers of inflammation significantly changed during the time course.

Conclusions: The administration of bacterial endotoxin induced a significant rise in degradation products of the endothelial glycocalyx.

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Time course of serum heparan sulfate concentrations after administration of endotoxin (T0). Endotoxin group: #: p < 0.001 for T2 versus T0; p < 0.001 for T2 versus T1. Comparison between groups: $: p < 0.001 for T2. T0: Baseline, immediately before endotoxin administration; T1: 3 h after endotoxin administration; T2: 6 h after endotoxin administration. Values are mean ± SD.
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Figure 1: Time course of serum heparan sulfate concentrations after administration of endotoxin (T0). Endotoxin group: #: p < 0.001 for T2 versus T0; p < 0.001 for T2 versus T1. Comparison between groups: $: p < 0.001 for T2. T0: Baseline, immediately before endotoxin administration; T1: 3 h after endotoxin administration; T2: 6 h after endotoxin administration. Values are mean ± SD.

Mentions: Serum HS concentrations did not change in the CG during the observation period, but significantly increased over time in the EG (p < 0.001, T2). (Figure 1).


Serum heparan sulfate levels are elevated in endotoxemia.

Hofmann-Kiefer KF, Kemming GI, Chappell D, Flondor M, Kisch-Wedel H, Hauser A, Pallivathukal S, Conzen P, Rehm M - Eur. J. Med. Res. (2009)

Time course of serum heparan sulfate concentrations after administration of endotoxin (T0). Endotoxin group: #: p < 0.001 for T2 versus T0; p < 0.001 for T2 versus T1. Comparison between groups: $: p < 0.001 for T2. T0: Baseline, immediately before endotoxin administration; T1: 3 h after endotoxin administration; T2: 6 h after endotoxin administration. Values are mean ± SD.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3351938&req=5

Figure 1: Time course of serum heparan sulfate concentrations after administration of endotoxin (T0). Endotoxin group: #: p < 0.001 for T2 versus T0; p < 0.001 for T2 versus T1. Comparison between groups: $: p < 0.001 for T2. T0: Baseline, immediately before endotoxin administration; T1: 3 h after endotoxin administration; T2: 6 h after endotoxin administration. Values are mean ± SD.
Mentions: Serum HS concentrations did not change in the CG during the observation period, but significantly increased over time in the EG (p < 0.001, T2). (Figure 1).

Bottom Line: Serum heparan sulfate concentrations significantly increased over time in the endotoxin group and were significantly elevated in comparison to the control group 6 hours after administration of endotoxin (p<0.001).In the endotoxin group all markers of inflammation significantly changed during the time course.The administration of bacterial endotoxin induced a significant rise in degradation products of the endothelial glycocalyx.

View Article: PubMed Central - HTML - PubMed

Affiliation: Clinic of Anesthesiology/Critical Care Medicine and Pain Therapy (M.A.B., P.C), Ludwig-Maximilians-University, City of Munich, Germany. Klaus.Hofmann-Kiefer@med.uni-muenchen.de

ABSTRACT

Background: Increased vascular permeability is a characteristic feature of sepsis which, in the past, has been ascribed exclusively to a malfunction of endothelial cells. However, recently it has become evident that the endothelial glycocalyx is of considerable importance concerning various aspects of vascular physiology, e.g. the vascular barrier and inflammation. Heparan sulfate, one of its essential components is characteristically traceable in blood, in case the endothelial glycocalyx is damaged or destroyed.

Methods: In 15 pigs we investigated whether the administration of endotoxin from gram-negative bacteria (Escherichia coli) results in increased serum levels of heparan sulfate, signalizing a shedding of the glycocalyx. In addition, markers of inflammation (white blood cell count, platelet count, tumour necrosis factor-α and interleukin-6) were evaluated over an observation period of 6 hours.

Results: Serum heparan sulfate concentrations significantly increased over time in the endotoxin group and were significantly elevated in comparison to the control group 6 hours after administration of endotoxin (p<0.001). In the endotoxin group all markers of inflammation significantly changed during the time course.

Conclusions: The administration of bacterial endotoxin induced a significant rise in degradation products of the endothelial glycocalyx.

Show MeSH
Related in: MedlinePlus