Limits...
Simian virus 40 inhibits differentiation and maturation of rhesus macaque DC-SIGN(+) dendritic cells.

Changyong C, Sun M, Li H, Brockmeyer N, Wu NP - Eur. J. Med. Res. (2010)

Bottom Line: Dendritic cells (DC) are the initiators and modulators of the immune responses.Results showed that SV40 can down-regulate the expression of CD83 and CD86 on DC and impair DC-induced activation of T cell proliferation.These findings suggest that SV40 might also cause immune suppression by influencing differentiation and maturation of DC.

View Article: PubMed Central - HTML - PubMed

Affiliation: The State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou, PR China.

ABSTRACT
Dendritic cells (DC) are the initiators and modulators of the immune responses. Some species of pathogenic microorganisms have developed immune evasion strategies by controlling antigen presentation function of DC. Simian virus 40 (SV40) is a DNA tumor virus of rhesus monkey origin. It can induce cell transformation and tumorigenesis in many vertebrate species, but often causes no visible effects and persists as a latent infection in rhesus monkeys under natural conditions. To investigate the interaction between SV40 and rhesus monkey DC, rhesus monkey peripheral blood monocyte-derived DC were induced using recombinant human Interleukin-4 (rhIL-4) and infective SV40, the phenotype and function of DC-specific intracellular adhesion molecule-3 grabbing nonintegrin (DC-SIGN)(+) DC were analyzed by flow cytometry (FCM) and mixed lymphocyte reaction (MLR). Results showed that SV40 can down-regulate the expression of CD83 and CD86 on DC and impair DC-induced activation of T cell proliferation. These findings suggest that SV40 might also cause immune suppression by influencing differentiation and maturation of DC.

Show MeSH

Related in: MedlinePlus

Expression of CD83, CD209 on DC cell surface of antigen-untreated group. (A) Expression of CD83 on DC cell surface of antigen-untreated group at day 9 culture (the dotted line, isotype control; the solid line, CD83 labeling). (B) Expression of CD209 on DC cell surface of antigen-untreated group at day 9 culture (the dotted line, isotype control; the solid line, CD209 labeling).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3351904&req=5

Figure 2: Expression of CD83, CD209 on DC cell surface of antigen-untreated group. (A) Expression of CD83 on DC cell surface of antigen-untreated group at day 9 culture (the dotted line, isotype control; the solid line, CD83 labeling). (B) Expression of CD209 on DC cell surface of antigen-untreated group at day 9 culture (the dotted line, isotype control; the solid line, CD209 labeling).

Mentions: The expression of CD83, CD209 on rhesus macaque DC cell surface of antigen-untreated group was analyzed by flow cytometric analysis. The results showed that peripheral blood monocyte-derived DC cultured with GM-CSF and IL-4 express CD83 and CD209 on cell surface. At day 9 of cell culture, the percentage of DC expressing CD83 and CD209 is 10.8 ± 2.3% and 69.7 ± 5.2%, respectively (Figure 2).


Simian virus 40 inhibits differentiation and maturation of rhesus macaque DC-SIGN(+) dendritic cells.

Changyong C, Sun M, Li H, Brockmeyer N, Wu NP - Eur. J. Med. Res. (2010)

Expression of CD83, CD209 on DC cell surface of antigen-untreated group. (A) Expression of CD83 on DC cell surface of antigen-untreated group at day 9 culture (the dotted line, isotype control; the solid line, CD83 labeling). (B) Expression of CD209 on DC cell surface of antigen-untreated group at day 9 culture (the dotted line, isotype control; the solid line, CD209 labeling).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3351904&req=5

Figure 2: Expression of CD83, CD209 on DC cell surface of antigen-untreated group. (A) Expression of CD83 on DC cell surface of antigen-untreated group at day 9 culture (the dotted line, isotype control; the solid line, CD83 labeling). (B) Expression of CD209 on DC cell surface of antigen-untreated group at day 9 culture (the dotted line, isotype control; the solid line, CD209 labeling).
Mentions: The expression of CD83, CD209 on rhesus macaque DC cell surface of antigen-untreated group was analyzed by flow cytometric analysis. The results showed that peripheral blood monocyte-derived DC cultured with GM-CSF and IL-4 express CD83 and CD209 on cell surface. At day 9 of cell culture, the percentage of DC expressing CD83 and CD209 is 10.8 ± 2.3% and 69.7 ± 5.2%, respectively (Figure 2).

Bottom Line: Dendritic cells (DC) are the initiators and modulators of the immune responses.Results showed that SV40 can down-regulate the expression of CD83 and CD86 on DC and impair DC-induced activation of T cell proliferation.These findings suggest that SV40 might also cause immune suppression by influencing differentiation and maturation of DC.

View Article: PubMed Central - HTML - PubMed

Affiliation: The State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou, PR China.

ABSTRACT
Dendritic cells (DC) are the initiators and modulators of the immune responses. Some species of pathogenic microorganisms have developed immune evasion strategies by controlling antigen presentation function of DC. Simian virus 40 (SV40) is a DNA tumor virus of rhesus monkey origin. It can induce cell transformation and tumorigenesis in many vertebrate species, but often causes no visible effects and persists as a latent infection in rhesus monkeys under natural conditions. To investigate the interaction between SV40 and rhesus monkey DC, rhesus monkey peripheral blood monocyte-derived DC were induced using recombinant human Interleukin-4 (rhIL-4) and infective SV40, the phenotype and function of DC-specific intracellular adhesion molecule-3 grabbing nonintegrin (DC-SIGN)(+) DC were analyzed by flow cytometry (FCM) and mixed lymphocyte reaction (MLR). Results showed that SV40 can down-regulate the expression of CD83 and CD86 on DC and impair DC-induced activation of T cell proliferation. These findings suggest that SV40 might also cause immune suppression by influencing differentiation and maturation of DC.

Show MeSH
Related in: MedlinePlus