Limits...
Simian virus 40 inhibits differentiation and maturation of rhesus macaque DC-SIGN(+) dendritic cells.

Changyong C, Sun M, Li H, Brockmeyer N, Wu NP - Eur. J. Med. Res. (2010)

Bottom Line: Dendritic cells (DC) are the initiators and modulators of the immune responses.Results showed that SV40 can down-regulate the expression of CD83 and CD86 on DC and impair DC-induced activation of T cell proliferation.These findings suggest that SV40 might also cause immune suppression by influencing differentiation and maturation of DC.

View Article: PubMed Central - HTML - PubMed

Affiliation: The State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou, PR China.

ABSTRACT
Dendritic cells (DC) are the initiators and modulators of the immune responses. Some species of pathogenic microorganisms have developed immune evasion strategies by controlling antigen presentation function of DC. Simian virus 40 (SV40) is a DNA tumor virus of rhesus monkey origin. It can induce cell transformation and tumorigenesis in many vertebrate species, but often causes no visible effects and persists as a latent infection in rhesus monkeys under natural conditions. To investigate the interaction between SV40 and rhesus monkey DC, rhesus monkey peripheral blood monocyte-derived DC were induced using recombinant human Interleukin-4 (rhIL-4) and infective SV40, the phenotype and function of DC-specific intracellular adhesion molecule-3 grabbing nonintegrin (DC-SIGN)(+) DC were analyzed by flow cytometry (FCM) and mixed lymphocyte reaction (MLR). Results showed that SV40 can down-regulate the expression of CD83 and CD86 on DC and impair DC-induced activation of T cell proliferation. These findings suggest that SV40 might also cause immune suppression by influencing differentiation and maturation of DC.

Show MeSH

Related in: MedlinePlus

Morphology of rhesus macaque monocyte-derived DC. (A) Cell colonies of dendritic cell precursors of antigen-untreated group on day 3 of culture (magnification, × 400). (B) Morphology of DC of inactivated SV40-treated group on day 9 of culture (magnification, × 400). (C) Morphology of DC of inactivated SV40-treated group on day 12 of culture (magnification, × 2500). (D) Cell colonies of dendritic cell precursors of antigen-untreated group on day 9 of culture (magnification, × 400). (E) Morphology of DC of infective SV40-treated group on day 9 of culture (magnification, × 400). (F) Morphology of DC of infective SV40-treated group on day 12 of culture (magnification, × 1200).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3351904&req=5

Figure 1: Morphology of rhesus macaque monocyte-derived DC. (A) Cell colonies of dendritic cell precursors of antigen-untreated group on day 3 of culture (magnification, × 400). (B) Morphology of DC of inactivated SV40-treated group on day 9 of culture (magnification, × 400). (C) Morphology of DC of inactivated SV40-treated group on day 12 of culture (magnification, × 2500). (D) Cell colonies of dendritic cell precursors of antigen-untreated group on day 9 of culture (magnification, × 400). (E) Morphology of DC of infective SV40-treated group on day 9 of culture (magnification, × 400). (F) Morphology of DC of infective SV40-treated group on day 12 of culture (magnification, × 1200).

Mentions: Rhesus macaque monocytes were proliferated by using RPMI 1640 medium supplemented with 500 ng/ml rhGM-CSF. IL-4 which induces monocytes to differentiate toward DDC was added to culture medium after 7 days of culture, and then infective SV40 and inactivated SV40 were added to culture medium after 3 days of IL-4 inducement culture respectively. After additional 9 days of culture, a great number of spiny, crab-like, pompon-like, stellate cells were seen floating in the culture medium (Figure 1B, E). At day 12 of culture, these monocyte-derived dendritic cells, which mainly exhibit squamous, petallike, veillike, sheet-like processes, could be observed under scanning electron microscope (Figure 1C, F). Morphocytological changes of experiment group (infective SV40-treated group) are similar to that of negative control group (inactivated SV40-treated group). The morphology of blank control group (antigen-untreated group), in contrast, remained unchanged all the time (Figure 1A, D).


Simian virus 40 inhibits differentiation and maturation of rhesus macaque DC-SIGN(+) dendritic cells.

Changyong C, Sun M, Li H, Brockmeyer N, Wu NP - Eur. J. Med. Res. (2010)

Morphology of rhesus macaque monocyte-derived DC. (A) Cell colonies of dendritic cell precursors of antigen-untreated group on day 3 of culture (magnification, × 400). (B) Morphology of DC of inactivated SV40-treated group on day 9 of culture (magnification, × 400). (C) Morphology of DC of inactivated SV40-treated group on day 12 of culture (magnification, × 2500). (D) Cell colonies of dendritic cell precursors of antigen-untreated group on day 9 of culture (magnification, × 400). (E) Morphology of DC of infective SV40-treated group on day 9 of culture (magnification, × 400). (F) Morphology of DC of infective SV40-treated group on day 12 of culture (magnification, × 1200).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3351904&req=5

Figure 1: Morphology of rhesus macaque monocyte-derived DC. (A) Cell colonies of dendritic cell precursors of antigen-untreated group on day 3 of culture (magnification, × 400). (B) Morphology of DC of inactivated SV40-treated group on day 9 of culture (magnification, × 400). (C) Morphology of DC of inactivated SV40-treated group on day 12 of culture (magnification, × 2500). (D) Cell colonies of dendritic cell precursors of antigen-untreated group on day 9 of culture (magnification, × 400). (E) Morphology of DC of infective SV40-treated group on day 9 of culture (magnification, × 400). (F) Morphology of DC of infective SV40-treated group on day 12 of culture (magnification, × 1200).
Mentions: Rhesus macaque monocytes were proliferated by using RPMI 1640 medium supplemented with 500 ng/ml rhGM-CSF. IL-4 which induces monocytes to differentiate toward DDC was added to culture medium after 7 days of culture, and then infective SV40 and inactivated SV40 were added to culture medium after 3 days of IL-4 inducement culture respectively. After additional 9 days of culture, a great number of spiny, crab-like, pompon-like, stellate cells were seen floating in the culture medium (Figure 1B, E). At day 12 of culture, these monocyte-derived dendritic cells, which mainly exhibit squamous, petallike, veillike, sheet-like processes, could be observed under scanning electron microscope (Figure 1C, F). Morphocytological changes of experiment group (infective SV40-treated group) are similar to that of negative control group (inactivated SV40-treated group). The morphology of blank control group (antigen-untreated group), in contrast, remained unchanged all the time (Figure 1A, D).

Bottom Line: Dendritic cells (DC) are the initiators and modulators of the immune responses.Results showed that SV40 can down-regulate the expression of CD83 and CD86 on DC and impair DC-induced activation of T cell proliferation.These findings suggest that SV40 might also cause immune suppression by influencing differentiation and maturation of DC.

View Article: PubMed Central - HTML - PubMed

Affiliation: The State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou, PR China.

ABSTRACT
Dendritic cells (DC) are the initiators and modulators of the immune responses. Some species of pathogenic microorganisms have developed immune evasion strategies by controlling antigen presentation function of DC. Simian virus 40 (SV40) is a DNA tumor virus of rhesus monkey origin. It can induce cell transformation and tumorigenesis in many vertebrate species, but often causes no visible effects and persists as a latent infection in rhesus monkeys under natural conditions. To investigate the interaction between SV40 and rhesus monkey DC, rhesus monkey peripheral blood monocyte-derived DC were induced using recombinant human Interleukin-4 (rhIL-4) and infective SV40, the phenotype and function of DC-specific intracellular adhesion molecule-3 grabbing nonintegrin (DC-SIGN)(+) DC were analyzed by flow cytometry (FCM) and mixed lymphocyte reaction (MLR). Results showed that SV40 can down-regulate the expression of CD83 and CD86 on DC and impair DC-induced activation of T cell proliferation. These findings suggest that SV40 might also cause immune suppression by influencing differentiation and maturation of DC.

Show MeSH
Related in: MedlinePlus