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Immunohistochemical expression of heat shock protein27 in the mouse dental pulp after immediate teeth separation.

Saito S, Nakano K, Nabeyama A, Sato M, Okafuji N, Yamamoto A, Kasahara E, Kawakami T - Eur. J. Med. Res. (2011)

Bottom Line: In both 30 min and 3 h experimental groups, HSP27 expression was highest at 24 h after wedge removal and the expression remained the same or started to decrease thereafter.The expression decreased at the same level as that of the control group 1 week after wedge removal.HSP27 may serve as an indicator of stimulus strong enough to show its expression.

View Article: PubMed Central - HTML - PubMed

Affiliation: Matsumoto Dental University, Graduate School of Oral Medicine, Shiojiri, Japan.

ABSTRACT

Aim: After immediate teeth separation, expression of HSP27 in the mouse dental pulp was examined. Immunohistochemistry was performed to examine the incidence of HSP27 expression.

Materials and methods: A total of 36 8-week-old ddY mice were used as experimental subjects and a wedge was inserted in between maxillary right molars. The wedge was removed 30 min or 3 h after insertion. Animals were immediately sacrificed after the removal of wedge or until 1 week later and serial sections from paraffin-embedded tissues were prepared. Immunohistochemistry was carried out to examine the expression of HSP27. The untreated side served as the control.

Results: In the control group, the endothelial cells and some pulp fibroblasts weakly expressed HSP27 suggesting that the expression is due to mechanical stress brought about by physiological masticatory force and pressure from the tongue. In both 30 min and 3 h experimental groups, HSP27 expression was highest at 24 h after wedge removal and the expression remained the same or started to decrease thereafter. The expression decreased at the same level as that of the control group 1 week after wedge removal.

Conclusion: HSP27 may serve as an indicator of stimulus strong enough to show its expression.

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Related in: MedlinePlus

The positive reactions appeared in almost all dental pulp tissue cells (a: 3 h-0 m, Bar 20 μm); staining reaction become strong (b: 3 h-3 h, Bar 20 μm), the strong positive reactions are observed in pulp cells (c: 3 h-24 h, Bar 20 μm), strong positive reaction in the pulp cells (d: 3 h-1 w, Bar 20 μm).
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Figure 4: The positive reactions appeared in almost all dental pulp tissue cells (a: 3 h-0 m, Bar 20 μm); staining reaction become strong (b: 3 h-3 h, Bar 20 μm), the strong positive reactions are observed in pulp cells (c: 3 h-24 h, Bar 20 μm), strong positive reaction in the pulp cells (d: 3 h-1 w, Bar 20 μm).

Mentions: In 3 h group, HSP27 expression in the cytoplasm of endothelial cells became stronger (Figure 3-b). In 9 h group, HSP27 became stronger and is expressed in the cytoplasm of endothelial cells as well as in some fibroblasts in the coronal pulp. In 24 h group, HSP27 expression remained the same with 9 h group (Figure 3c). In 3 days group, HSP27 expression in the odontoblast layer can still be observed but a decrease in expression is evident. In 1 week group, HSP27 further decreased with no difference with the control group (Figure 4d).


Immunohistochemical expression of heat shock protein27 in the mouse dental pulp after immediate teeth separation.

Saito S, Nakano K, Nabeyama A, Sato M, Okafuji N, Yamamoto A, Kasahara E, Kawakami T - Eur. J. Med. Res. (2011)

The positive reactions appeared in almost all dental pulp tissue cells (a: 3 h-0 m, Bar 20 μm); staining reaction become strong (b: 3 h-3 h, Bar 20 μm), the strong positive reactions are observed in pulp cells (c: 3 h-24 h, Bar 20 μm), strong positive reaction in the pulp cells (d: 3 h-1 w, Bar 20 μm).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3351807&req=5

Figure 4: The positive reactions appeared in almost all dental pulp tissue cells (a: 3 h-0 m, Bar 20 μm); staining reaction become strong (b: 3 h-3 h, Bar 20 μm), the strong positive reactions are observed in pulp cells (c: 3 h-24 h, Bar 20 μm), strong positive reaction in the pulp cells (d: 3 h-1 w, Bar 20 μm).
Mentions: In 3 h group, HSP27 expression in the cytoplasm of endothelial cells became stronger (Figure 3-b). In 9 h group, HSP27 became stronger and is expressed in the cytoplasm of endothelial cells as well as in some fibroblasts in the coronal pulp. In 24 h group, HSP27 expression remained the same with 9 h group (Figure 3c). In 3 days group, HSP27 expression in the odontoblast layer can still be observed but a decrease in expression is evident. In 1 week group, HSP27 further decreased with no difference with the control group (Figure 4d).

Bottom Line: In both 30 min and 3 h experimental groups, HSP27 expression was highest at 24 h after wedge removal and the expression remained the same or started to decrease thereafter.The expression decreased at the same level as that of the control group 1 week after wedge removal.HSP27 may serve as an indicator of stimulus strong enough to show its expression.

View Article: PubMed Central - HTML - PubMed

Affiliation: Matsumoto Dental University, Graduate School of Oral Medicine, Shiojiri, Japan.

ABSTRACT

Aim: After immediate teeth separation, expression of HSP27 in the mouse dental pulp was examined. Immunohistochemistry was performed to examine the incidence of HSP27 expression.

Materials and methods: A total of 36 8-week-old ddY mice were used as experimental subjects and a wedge was inserted in between maxillary right molars. The wedge was removed 30 min or 3 h after insertion. Animals were immediately sacrificed after the removal of wedge or until 1 week later and serial sections from paraffin-embedded tissues were prepared. Immunohistochemistry was carried out to examine the expression of HSP27. The untreated side served as the control.

Results: In the control group, the endothelial cells and some pulp fibroblasts weakly expressed HSP27 suggesting that the expression is due to mechanical stress brought about by physiological masticatory force and pressure from the tongue. In both 30 min and 3 h experimental groups, HSP27 expression was highest at 24 h after wedge removal and the expression remained the same or started to decrease thereafter. The expression decreased at the same level as that of the control group 1 week after wedge removal.

Conclusion: HSP27 may serve as an indicator of stimulus strong enough to show its expression.

Show MeSH
Related in: MedlinePlus