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Immunohistochemical expression of heat shock protein27 in the mouse dental pulp after immediate teeth separation.

Saito S, Nakano K, Nabeyama A, Sato M, Okafuji N, Yamamoto A, Kasahara E, Kawakami T - Eur. J. Med. Res. (2011)

Bottom Line: In both 30 min and 3 h experimental groups, HSP27 expression was highest at 24 h after wedge removal and the expression remained the same or started to decrease thereafter.The expression decreased at the same level as that of the control group 1 week after wedge removal.HSP27 may serve as an indicator of stimulus strong enough to show its expression.

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Affiliation: Matsumoto Dental University, Graduate School of Oral Medicine, Shiojiri, Japan.

ABSTRACT

Aim: After immediate teeth separation, expression of HSP27 in the mouse dental pulp was examined. Immunohistochemistry was performed to examine the incidence of HSP27 expression.

Materials and methods: A total of 36 8-week-old ddY mice were used as experimental subjects and a wedge was inserted in between maxillary right molars. The wedge was removed 30 min or 3 h after insertion. Animals were immediately sacrificed after the removal of wedge or until 1 week later and serial sections from paraffin-embedded tissues were prepared. Immunohistochemistry was carried out to examine the expression of HSP27. The untreated side served as the control.

Results: In the control group, the endothelial cells and some pulp fibroblasts weakly expressed HSP27 suggesting that the expression is due to mechanical stress brought about by physiological masticatory force and pressure from the tongue. In both 30 min and 3 h experimental groups, HSP27 expression was highest at 24 h after wedge removal and the expression remained the same or started to decrease thereafter. The expression decreased at the same level as that of the control group 1 week after wedge removal.

Conclusion: HSP27 may serve as an indicator of stimulus strong enough to show its expression.

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Related in: MedlinePlus

HSP27 positive staining appeared in some endothelial cells (control, Bar 20 μm).
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Figure 2: HSP27 positive staining appeared in some endothelial cells (control, Bar 20 μm).

Mentions: HSP27 is weakly expressed in the cytoplasm of endothelial cells in the odontoblast layer (Figure 2).


Immunohistochemical expression of heat shock protein27 in the mouse dental pulp after immediate teeth separation.

Saito S, Nakano K, Nabeyama A, Sato M, Okafuji N, Yamamoto A, Kasahara E, Kawakami T - Eur. J. Med. Res. (2011)

HSP27 positive staining appeared in some endothelial cells (control, Bar 20 μm).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3351807&req=5

Figure 2: HSP27 positive staining appeared in some endothelial cells (control, Bar 20 μm).
Mentions: HSP27 is weakly expressed in the cytoplasm of endothelial cells in the odontoblast layer (Figure 2).

Bottom Line: In both 30 min and 3 h experimental groups, HSP27 expression was highest at 24 h after wedge removal and the expression remained the same or started to decrease thereafter.The expression decreased at the same level as that of the control group 1 week after wedge removal.HSP27 may serve as an indicator of stimulus strong enough to show its expression.

View Article: PubMed Central - HTML - PubMed

Affiliation: Matsumoto Dental University, Graduate School of Oral Medicine, Shiojiri, Japan.

ABSTRACT

Aim: After immediate teeth separation, expression of HSP27 in the mouse dental pulp was examined. Immunohistochemistry was performed to examine the incidence of HSP27 expression.

Materials and methods: A total of 36 8-week-old ddY mice were used as experimental subjects and a wedge was inserted in between maxillary right molars. The wedge was removed 30 min or 3 h after insertion. Animals were immediately sacrificed after the removal of wedge or until 1 week later and serial sections from paraffin-embedded tissues were prepared. Immunohistochemistry was carried out to examine the expression of HSP27. The untreated side served as the control.

Results: In the control group, the endothelial cells and some pulp fibroblasts weakly expressed HSP27 suggesting that the expression is due to mechanical stress brought about by physiological masticatory force and pressure from the tongue. In both 30 min and 3 h experimental groups, HSP27 expression was highest at 24 h after wedge removal and the expression remained the same or started to decrease thereafter. The expression decreased at the same level as that of the control group 1 week after wedge removal.

Conclusion: HSP27 may serve as an indicator of stimulus strong enough to show its expression.

Show MeSH
Related in: MedlinePlus