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Immunohistochemical expression of heat shock protein27 in the mouse dental pulp after immediate teeth separation.

Saito S, Nakano K, Nabeyama A, Sato M, Okafuji N, Yamamoto A, Kasahara E, Kawakami T - Eur. J. Med. Res. (2011)

Bottom Line: In both 30 min and 3 h experimental groups, HSP27 expression was highest at 24 h after wedge removal and the expression remained the same or started to decrease thereafter.The expression decreased at the same level as that of the control group 1 week after wedge removal.HSP27 may serve as an indicator of stimulus strong enough to show its expression.

View Article: PubMed Central - HTML - PubMed

Affiliation: Matsumoto Dental University, Graduate School of Oral Medicine, Shiojiri, Japan.

ABSTRACT

Aim: After immediate teeth separation, expression of HSP27 in the mouse dental pulp was examined. Immunohistochemistry was performed to examine the incidence of HSP27 expression.

Materials and methods: A total of 36 8-week-old ddY mice were used as experimental subjects and a wedge was inserted in between maxillary right molars. The wedge was removed 30 min or 3 h after insertion. Animals were immediately sacrificed after the removal of wedge or until 1 week later and serial sections from paraffin-embedded tissues were prepared. Immunohistochemistry was carried out to examine the expression of HSP27. The untreated side served as the control.

Results: In the control group, the endothelial cells and some pulp fibroblasts weakly expressed HSP27 suggesting that the expression is due to mechanical stress brought about by physiological masticatory force and pressure from the tongue. In both 30 min and 3 h experimental groups, HSP27 expression was highest at 24 h after wedge removal and the expression remained the same or started to decrease thereafter. The expression decreased at the same level as that of the control group 1 week after wedge removal.

Conclusion: HSP27 may serve as an indicator of stimulus strong enough to show its expression.

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Histopathological view of the horizontal section of a sample from the experimental group (a: Bar 200 μm), immunohistochemical staining profile of HSP27 in the 1st molar, experimental group (b: 3 h-3 h, Bar 100 μm).
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Figure 1: Histopathological view of the horizontal section of a sample from the experimental group (a: Bar 200 μm), immunohistochemical staining profile of HSP27 in the 1st molar, experimental group (b: 3 h-3 h, Bar 100 μm).

Mentions: Prior to the experiment, the mice were subjected to inhalation anesthesia with isoflurane (ISOFLU: Dainippon Sumitomo Pharma Co. Animal Science, Osaka, Japan) and gas air mixture (4.0% pre-concentration of anesthesia). The experiments were performed under a stable anesthetic concentration with adjustable constant flow rate of gas anesthesia system for small laboratory animal (DS Pharma Biomedical Co. Ltd, Laboratory products, Osaka, Japan). After induction of anesthesia, the upper body of the mouse was fixed in a homemade bench. Isoflurance inhalation anesthesia was allowed to flow and maintained during the experiment by inserting a suction hole in the nose from time to time (1.0% maintenance concentration). In order to keep the mouth open during the experiment, the jaw was fixed with a thread tied on the upper incisor from the top of the bench and the lower jaw was hung and fixed on the lower bench with a thread tied on the lower incisors. Then after a wedge (Anatomical WIZARD WEDGES: Water Pik, Inc. Ft. Collins, Colorado, U.S.A.) was inserted in between maxillary molars. The teeth included in the sections are the following: maxillary first molar (M1), maxillary second molar (M2), maxillary third molar (M3) where M1 has 3 roots, M2 has 3 roots and M3 has 2 to 3 roots (Figure 1-a). The wedge was inserted in between the right maxillary first (M1) and second (M2) molars. The experimental groups were first divided into 2 groups: 30 min and 3 h, the time where in the wedge was left inserted in between teeth (separation time). Then, the groups were further divided 0 min, 3 h, 9 h, 24 h, 3 days, 1 week as the time after the removal of the wedge until the mouse was sacrificed for histological examination. A total of 12 experimental groups were formed. The number of samples in each group is shown on Table 1. When the duration of anesthesia has lapsed after each experiment, isoflurane inhalation was similarly given. The tissue sample excised consists of a portion of periodontal tissue of the maxillary molars.


Immunohistochemical expression of heat shock protein27 in the mouse dental pulp after immediate teeth separation.

Saito S, Nakano K, Nabeyama A, Sato M, Okafuji N, Yamamoto A, Kasahara E, Kawakami T - Eur. J. Med. Res. (2011)

Histopathological view of the horizontal section of a sample from the experimental group (a: Bar 200 μm), immunohistochemical staining profile of HSP27 in the 1st molar, experimental group (b: 3 h-3 h, Bar 100 μm).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3351807&req=5

Figure 1: Histopathological view of the horizontal section of a sample from the experimental group (a: Bar 200 μm), immunohistochemical staining profile of HSP27 in the 1st molar, experimental group (b: 3 h-3 h, Bar 100 μm).
Mentions: Prior to the experiment, the mice were subjected to inhalation anesthesia with isoflurane (ISOFLU: Dainippon Sumitomo Pharma Co. Animal Science, Osaka, Japan) and gas air mixture (4.0% pre-concentration of anesthesia). The experiments were performed under a stable anesthetic concentration with adjustable constant flow rate of gas anesthesia system for small laboratory animal (DS Pharma Biomedical Co. Ltd, Laboratory products, Osaka, Japan). After induction of anesthesia, the upper body of the mouse was fixed in a homemade bench. Isoflurance inhalation anesthesia was allowed to flow and maintained during the experiment by inserting a suction hole in the nose from time to time (1.0% maintenance concentration). In order to keep the mouth open during the experiment, the jaw was fixed with a thread tied on the upper incisor from the top of the bench and the lower jaw was hung and fixed on the lower bench with a thread tied on the lower incisors. Then after a wedge (Anatomical WIZARD WEDGES: Water Pik, Inc. Ft. Collins, Colorado, U.S.A.) was inserted in between maxillary molars. The teeth included in the sections are the following: maxillary first molar (M1), maxillary second molar (M2), maxillary third molar (M3) where M1 has 3 roots, M2 has 3 roots and M3 has 2 to 3 roots (Figure 1-a). The wedge was inserted in between the right maxillary first (M1) and second (M2) molars. The experimental groups were first divided into 2 groups: 30 min and 3 h, the time where in the wedge was left inserted in between teeth (separation time). Then, the groups were further divided 0 min, 3 h, 9 h, 24 h, 3 days, 1 week as the time after the removal of the wedge until the mouse was sacrificed for histological examination. A total of 12 experimental groups were formed. The number of samples in each group is shown on Table 1. When the duration of anesthesia has lapsed after each experiment, isoflurane inhalation was similarly given. The tissue sample excised consists of a portion of periodontal tissue of the maxillary molars.

Bottom Line: In both 30 min and 3 h experimental groups, HSP27 expression was highest at 24 h after wedge removal and the expression remained the same or started to decrease thereafter.The expression decreased at the same level as that of the control group 1 week after wedge removal.HSP27 may serve as an indicator of stimulus strong enough to show its expression.

View Article: PubMed Central - HTML - PubMed

Affiliation: Matsumoto Dental University, Graduate School of Oral Medicine, Shiojiri, Japan.

ABSTRACT

Aim: After immediate teeth separation, expression of HSP27 in the mouse dental pulp was examined. Immunohistochemistry was performed to examine the incidence of HSP27 expression.

Materials and methods: A total of 36 8-week-old ddY mice were used as experimental subjects and a wedge was inserted in between maxillary right molars. The wedge was removed 30 min or 3 h after insertion. Animals were immediately sacrificed after the removal of wedge or until 1 week later and serial sections from paraffin-embedded tissues were prepared. Immunohistochemistry was carried out to examine the expression of HSP27. The untreated side served as the control.

Results: In the control group, the endothelial cells and some pulp fibroblasts weakly expressed HSP27 suggesting that the expression is due to mechanical stress brought about by physiological masticatory force and pressure from the tongue. In both 30 min and 3 h experimental groups, HSP27 expression was highest at 24 h after wedge removal and the expression remained the same or started to decrease thereafter. The expression decreased at the same level as that of the control group 1 week after wedge removal.

Conclusion: HSP27 may serve as an indicator of stimulus strong enough to show its expression.

Show MeSH
Related in: MedlinePlus