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Study of T cell subsets and IL-7 protein expression in HIV-1-infected patients after 7 years HAART.

Shou C, Weng N, Jin Y, Feng L, Jin C, Hoextermann S, Potthoff A, Skaletz-Rorowski A, Brockmeyer NH, Wu N - Eur. J. Med. Res. (2011)

Bottom Line: At baseline CD4+ T cell counts of HIV-1-infected patients were lower than that in the control group (p < 0.01), whereas the CD8+, CD8/HLADR+ and CD8/CD38+ T cell counts were higher than those in the control group (p < 0.01).In the ineffective HAART group, the CD8/CD38+ T cell count had not decreased significantly, and CD8/HLADR+ T cell count gradually decreased.Before treatment, IL-7 serum levels of patients were significantly higher than that in the control group (p < 0.01).

View Article: PubMed Central - HTML - PubMed

Affiliation: Zhejiang University, School of Medicine, Hangzhou, China.

ABSTRACT

Objective: To study the changes in T cell subsets and IL-7 in HIV-1-infected patients after seven years of highly active antiretroviral therapy (HAART).

Methods: Seventy-five individuals were included in this study (25 with effective HAART, 18 with ineffective HAART, 17 untreated HIV+ patients, and 15 volunteers in the HIV negative control group). The counts of CD4+, CD8+, CD8/CD38+, and CD8/HLADR+ T cells as well as the IL-7 protein expression was measured at 5 time points during a period of seven years in patients starting HAART (baseline) and in the HIV negative control group. The expression of CD127 on CD3+ T cells was measured by flow cytometry at a single time point (after 7 years) in patients with HAART and was compared with untreated HIV+ patients and the HIV negative control group.

Results: At baseline CD4+ T cell counts of HIV-1-infected patients were lower than that in the control group (p < 0.01), whereas the CD8+, CD8/HLADR+ and CD8/CD38+ T cell counts were higher than those in the control group (p < 0.01). After seven years of effective HAART, the CD4+ T cell counts had increased and the CD8+ T cell count had decreased, although not to the normal levels (p < 0.05). Both the CD8/HLADR+ and CD8/CD38+ T cell counts had gradually approached those of the control group (p > 0.05). In the ineffective HAART group, the CD8/CD38+ T cell count had not decreased significantly, and CD8/HLADR+ T cell count gradually decreased. Before treatment, IL-7 serum levels of patients were significantly higher than that in the control group (p < 0.01). After seven years of effective HAART, IL-7 levels had gradually decreased, but were still higher than in the control group (p < 0.01). The CD127 expression on CD3+ CD8+ T cells in effective HAART patients was higher than in untreated HIV+ patients (p < 0.05), but was lower than that in the control group (p < 0.05). CD127 expression on CD3+ CD4+ T cells was not significantly different among the control group, untreated HIV+ patients and effective HAART group.

Conclusion: After seven years of effective HAART, the quantity and capacity of T cell subsets and IL-7 in HIV-1-infected patients had been partially restored, and the abnormal immune activation has significantly diminished.

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Difference of CD127 expression on CD3+ CD8+ T cell subsets in group A ( ± SD, %). Patient numbers: Control group (n = 15), Untreated group (n = 15), Effective HAART group (A) (n = 25). *: P < 0.05 compared with Control, **: P < 0.05 compared with untreated HIV+ patients.
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Figure 6: Difference of CD127 expression on CD3+ CD8+ T cell subsets in group A ( ± SD, %). Patient numbers: Control group (n = 15), Untreated group (n = 15), Effective HAART group (A) (n = 25). *: P < 0.05 compared with Control, **: P < 0.05 compared with untreated HIV+ patients.

Mentions: CD127 expression on the surface of CD3+ CD8+ T cells in untreated HIV-1-infected patients (group C) was lower than in the control group (43.5% vs 82.3%, p < 0.05). The CD127 expression in group A patients with effective HAART was higher than that in group C (59.4% vs 43.5%, p < 0.05), but still lower than that in the control group (59.4% vs 82.3%, p < 0.05). After seven years in group A, the expression level of CD127 on the surface of virgin (CD45RA+) CD3+ CD8+ T cells had returned close to that in the control group (83.8% vs 85.5%, p > 0.05). Although CD127 expression on memory (CD45RO+) CD3+ CD8+ T cells increased, it was still lower than that in the control group (55.2% vs 83.6%, p < 0.05) (Figure 6).


Study of T cell subsets and IL-7 protein expression in HIV-1-infected patients after 7 years HAART.

Shou C, Weng N, Jin Y, Feng L, Jin C, Hoextermann S, Potthoff A, Skaletz-Rorowski A, Brockmeyer NH, Wu N - Eur. J. Med. Res. (2011)

Difference of CD127 expression on CD3+ CD8+ T cell subsets in group A ( ± SD, %). Patient numbers: Control group (n = 15), Untreated group (n = 15), Effective HAART group (A) (n = 25). *: P < 0.05 compared with Control, **: P < 0.05 compared with untreated HIV+ patients.
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3351803&req=5

Figure 6: Difference of CD127 expression on CD3+ CD8+ T cell subsets in group A ( ± SD, %). Patient numbers: Control group (n = 15), Untreated group (n = 15), Effective HAART group (A) (n = 25). *: P < 0.05 compared with Control, **: P < 0.05 compared with untreated HIV+ patients.
Mentions: CD127 expression on the surface of CD3+ CD8+ T cells in untreated HIV-1-infected patients (group C) was lower than in the control group (43.5% vs 82.3%, p < 0.05). The CD127 expression in group A patients with effective HAART was higher than that in group C (59.4% vs 43.5%, p < 0.05), but still lower than that in the control group (59.4% vs 82.3%, p < 0.05). After seven years in group A, the expression level of CD127 on the surface of virgin (CD45RA+) CD3+ CD8+ T cells had returned close to that in the control group (83.8% vs 85.5%, p > 0.05). Although CD127 expression on memory (CD45RO+) CD3+ CD8+ T cells increased, it was still lower than that in the control group (55.2% vs 83.6%, p < 0.05) (Figure 6).

Bottom Line: At baseline CD4+ T cell counts of HIV-1-infected patients were lower than that in the control group (p < 0.01), whereas the CD8+, CD8/HLADR+ and CD8/CD38+ T cell counts were higher than those in the control group (p < 0.01).In the ineffective HAART group, the CD8/CD38+ T cell count had not decreased significantly, and CD8/HLADR+ T cell count gradually decreased.Before treatment, IL-7 serum levels of patients were significantly higher than that in the control group (p < 0.01).

View Article: PubMed Central - HTML - PubMed

Affiliation: Zhejiang University, School of Medicine, Hangzhou, China.

ABSTRACT

Objective: To study the changes in T cell subsets and IL-7 in HIV-1-infected patients after seven years of highly active antiretroviral therapy (HAART).

Methods: Seventy-five individuals were included in this study (25 with effective HAART, 18 with ineffective HAART, 17 untreated HIV+ patients, and 15 volunteers in the HIV negative control group). The counts of CD4+, CD8+, CD8/CD38+, and CD8/HLADR+ T cells as well as the IL-7 protein expression was measured at 5 time points during a period of seven years in patients starting HAART (baseline) and in the HIV negative control group. The expression of CD127 on CD3+ T cells was measured by flow cytometry at a single time point (after 7 years) in patients with HAART and was compared with untreated HIV+ patients and the HIV negative control group.

Results: At baseline CD4+ T cell counts of HIV-1-infected patients were lower than that in the control group (p < 0.01), whereas the CD8+, CD8/HLADR+ and CD8/CD38+ T cell counts were higher than those in the control group (p < 0.01). After seven years of effective HAART, the CD4+ T cell counts had increased and the CD8+ T cell count had decreased, although not to the normal levels (p < 0.05). Both the CD8/HLADR+ and CD8/CD38+ T cell counts had gradually approached those of the control group (p > 0.05). In the ineffective HAART group, the CD8/CD38+ T cell count had not decreased significantly, and CD8/HLADR+ T cell count gradually decreased. Before treatment, IL-7 serum levels of patients were significantly higher than that in the control group (p < 0.01). After seven years of effective HAART, IL-7 levels had gradually decreased, but were still higher than in the control group (p < 0.01). The CD127 expression on CD3+ CD8+ T cells in effective HAART patients was higher than in untreated HIV+ patients (p < 0.05), but was lower than that in the control group (p < 0.05). CD127 expression on CD3+ CD4+ T cells was not significantly different among the control group, untreated HIV+ patients and effective HAART group.

Conclusion: After seven years of effective HAART, the quantity and capacity of T cell subsets and IL-7 in HIV-1-infected patients had been partially restored, and the abnormal immune activation has significantly diminished.

Show MeSH
Related in: MedlinePlus