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Study of T cell subsets and IL-7 protein expression in HIV-1-infected patients after 7 years HAART.

Shou C, Weng N, Jin Y, Feng L, Jin C, Hoextermann S, Potthoff A, Skaletz-Rorowski A, Brockmeyer NH, Wu N - Eur. J. Med. Res. (2011)

Bottom Line: At baseline CD4+ T cell counts of HIV-1-infected patients were lower than that in the control group (p < 0.01), whereas the CD8+, CD8/HLADR+ and CD8/CD38+ T cell counts were higher than those in the control group (p < 0.01).In the ineffective HAART group, the CD8/CD38+ T cell count had not decreased significantly, and CD8/HLADR+ T cell count gradually decreased.Before treatment, IL-7 serum levels of patients were significantly higher than that in the control group (p < 0.01).

View Article: PubMed Central - HTML - PubMed

Affiliation: Zhejiang University, School of Medicine, Hangzhou, China.

ABSTRACT

Objective: To study the changes in T cell subsets and IL-7 in HIV-1-infected patients after seven years of highly active antiretroviral therapy (HAART).

Methods: Seventy-five individuals were included in this study (25 with effective HAART, 18 with ineffective HAART, 17 untreated HIV+ patients, and 15 volunteers in the HIV negative control group). The counts of CD4+, CD8+, CD8/CD38+, and CD8/HLADR+ T cells as well as the IL-7 protein expression was measured at 5 time points during a period of seven years in patients starting HAART (baseline) and in the HIV negative control group. The expression of CD127 on CD3+ T cells was measured by flow cytometry at a single time point (after 7 years) in patients with HAART and was compared with untreated HIV+ patients and the HIV negative control group.

Results: At baseline CD4+ T cell counts of HIV-1-infected patients were lower than that in the control group (p < 0.01), whereas the CD8+, CD8/HLADR+ and CD8/CD38+ T cell counts were higher than those in the control group (p < 0.01). After seven years of effective HAART, the CD4+ T cell counts had increased and the CD8+ T cell count had decreased, although not to the normal levels (p < 0.05). Both the CD8/HLADR+ and CD8/CD38+ T cell counts had gradually approached those of the control group (p > 0.05). In the ineffective HAART group, the CD8/CD38+ T cell count had not decreased significantly, and CD8/HLADR+ T cell count gradually decreased. Before treatment, IL-7 serum levels of patients were significantly higher than that in the control group (p < 0.01). After seven years of effective HAART, IL-7 levels had gradually decreased, but were still higher than in the control group (p < 0.01). The CD127 expression on CD3+ CD8+ T cells in effective HAART patients was higher than in untreated HIV+ patients (p < 0.05), but was lower than that in the control group (p < 0.05). CD127 expression on CD3+ CD4+ T cells was not significantly different among the control group, untreated HIV+ patients and effective HAART group.

Conclusion: After seven years of effective HAART, the quantity and capacity of T cell subsets and IL-7 in HIV-1-infected patients had been partially restored, and the abnormal immune activation has significantly diminished.

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Related in: MedlinePlus

Changes of IL-7 serum concentration during a period of seven years effective HAART (group A) ( ± SD, n = 25). *: P < 0.05 compared with Control.
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Figure 5: Changes of IL-7 serum concentration during a period of seven years effective HAART (group A) ( ± SD, n = 25). *: P < 0.05 compared with Control.

Mentions: Before treatment, the peripheral blood IL-7 levels in HIV-1-infected patients were significantly higher than in controls (29.5 vs 7.1 pg/mL, p < 0.01). Over the seven years of effective HAART, there was a gradual decrease in the levels of IL-7 (Figure 5). In the first year of HAART, the IL-7 level showed the largest decrease; however, by the seventh year of HAART, the IL-7 level was still higher than that in the controls (11.2 vs 7.1 pg/mL, p <0.05).


Study of T cell subsets and IL-7 protein expression in HIV-1-infected patients after 7 years HAART.

Shou C, Weng N, Jin Y, Feng L, Jin C, Hoextermann S, Potthoff A, Skaletz-Rorowski A, Brockmeyer NH, Wu N - Eur. J. Med. Res. (2011)

Changes of IL-7 serum concentration during a period of seven years effective HAART (group A) ( ± SD, n = 25). *: P < 0.05 compared with Control.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3351803&req=5

Figure 5: Changes of IL-7 serum concentration during a period of seven years effective HAART (group A) ( ± SD, n = 25). *: P < 0.05 compared with Control.
Mentions: Before treatment, the peripheral blood IL-7 levels in HIV-1-infected patients were significantly higher than in controls (29.5 vs 7.1 pg/mL, p < 0.01). Over the seven years of effective HAART, there was a gradual decrease in the levels of IL-7 (Figure 5). In the first year of HAART, the IL-7 level showed the largest decrease; however, by the seventh year of HAART, the IL-7 level was still higher than that in the controls (11.2 vs 7.1 pg/mL, p <0.05).

Bottom Line: At baseline CD4+ T cell counts of HIV-1-infected patients were lower than that in the control group (p < 0.01), whereas the CD8+, CD8/HLADR+ and CD8/CD38+ T cell counts were higher than those in the control group (p < 0.01).In the ineffective HAART group, the CD8/CD38+ T cell count had not decreased significantly, and CD8/HLADR+ T cell count gradually decreased.Before treatment, IL-7 serum levels of patients were significantly higher than that in the control group (p < 0.01).

View Article: PubMed Central - HTML - PubMed

Affiliation: Zhejiang University, School of Medicine, Hangzhou, China.

ABSTRACT

Objective: To study the changes in T cell subsets and IL-7 in HIV-1-infected patients after seven years of highly active antiretroviral therapy (HAART).

Methods: Seventy-five individuals were included in this study (25 with effective HAART, 18 with ineffective HAART, 17 untreated HIV+ patients, and 15 volunteers in the HIV negative control group). The counts of CD4+, CD8+, CD8/CD38+, and CD8/HLADR+ T cells as well as the IL-7 protein expression was measured at 5 time points during a period of seven years in patients starting HAART (baseline) and in the HIV negative control group. The expression of CD127 on CD3+ T cells was measured by flow cytometry at a single time point (after 7 years) in patients with HAART and was compared with untreated HIV+ patients and the HIV negative control group.

Results: At baseline CD4+ T cell counts of HIV-1-infected patients were lower than that in the control group (p < 0.01), whereas the CD8+, CD8/HLADR+ and CD8/CD38+ T cell counts were higher than those in the control group (p < 0.01). After seven years of effective HAART, the CD4+ T cell counts had increased and the CD8+ T cell count had decreased, although not to the normal levels (p < 0.05). Both the CD8/HLADR+ and CD8/CD38+ T cell counts had gradually approached those of the control group (p > 0.05). In the ineffective HAART group, the CD8/CD38+ T cell count had not decreased significantly, and CD8/HLADR+ T cell count gradually decreased. Before treatment, IL-7 serum levels of patients were significantly higher than that in the control group (p < 0.01). After seven years of effective HAART, IL-7 levels had gradually decreased, but were still higher than in the control group (p < 0.01). The CD127 expression on CD3+ CD8+ T cells in effective HAART patients was higher than in untreated HIV+ patients (p < 0.05), but was lower than that in the control group (p < 0.05). CD127 expression on CD3+ CD4+ T cells was not significantly different among the control group, untreated HIV+ patients and effective HAART group.

Conclusion: After seven years of effective HAART, the quantity and capacity of T cell subsets and IL-7 in HIV-1-infected patients had been partially restored, and the abnormal immune activation has significantly diminished.

Show MeSH
Related in: MedlinePlus