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Study of T cell subsets and IL-7 protein expression in HIV-1-infected patients after 7 years HAART.

Shou C, Weng N, Jin Y, Feng L, Jin C, Hoextermann S, Potthoff A, Skaletz-Rorowski A, Brockmeyer NH, Wu N - Eur. J. Med. Res. (2011)

Bottom Line: At baseline CD4+ T cell counts of HIV-1-infected patients were lower than that in the control group (p < 0.01), whereas the CD8+, CD8/HLADR+ and CD8/CD38+ T cell counts were higher than those in the control group (p < 0.01).In the ineffective HAART group, the CD8/CD38+ T cell count had not decreased significantly, and CD8/HLADR+ T cell count gradually decreased.Before treatment, IL-7 serum levels of patients were significantly higher than that in the control group (p < 0.01).

View Article: PubMed Central - HTML - PubMed

Affiliation: Zhejiang University, School of Medicine, Hangzhou, China.

ABSTRACT

Objective: To study the changes in T cell subsets and IL-7 in HIV-1-infected patients after seven years of highly active antiretroviral therapy (HAART).

Methods: Seventy-five individuals were included in this study (25 with effective HAART, 18 with ineffective HAART, 17 untreated HIV+ patients, and 15 volunteers in the HIV negative control group). The counts of CD4+, CD8+, CD8/CD38+, and CD8/HLADR+ T cells as well as the IL-7 protein expression was measured at 5 time points during a period of seven years in patients starting HAART (baseline) and in the HIV negative control group. The expression of CD127 on CD3+ T cells was measured by flow cytometry at a single time point (after 7 years) in patients with HAART and was compared with untreated HIV+ patients and the HIV negative control group.

Results: At baseline CD4+ T cell counts of HIV-1-infected patients were lower than that in the control group (p < 0.01), whereas the CD8+, CD8/HLADR+ and CD8/CD38+ T cell counts were higher than those in the control group (p < 0.01). After seven years of effective HAART, the CD4+ T cell counts had increased and the CD8+ T cell count had decreased, although not to the normal levels (p < 0.05). Both the CD8/HLADR+ and CD8/CD38+ T cell counts had gradually approached those of the control group (p > 0.05). In the ineffective HAART group, the CD8/CD38+ T cell count had not decreased significantly, and CD8/HLADR+ T cell count gradually decreased. Before treatment, IL-7 serum levels of patients were significantly higher than that in the control group (p < 0.01). After seven years of effective HAART, IL-7 levels had gradually decreased, but were still higher than in the control group (p < 0.01). The CD127 expression on CD3+ CD8+ T cells in effective HAART patients was higher than in untreated HIV+ patients (p < 0.05), but was lower than that in the control group (p < 0.05). CD127 expression on CD3+ CD4+ T cells was not significantly different among the control group, untreated HIV+ patients and effective HAART group.

Conclusion: After seven years of effective HAART, the quantity and capacity of T cell subsets and IL-7 in HIV-1-infected patients had been partially restored, and the abnormal immune activation has significantly diminished.

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Related in: MedlinePlus

Changes of CD4+ T and CD8+ T cells during a period of seven years effective HAART (group A) ( ± SD, n = 25). *: P < 0.05 compared with Control, **: P < 0.05 compared with Pretherapy.
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Figure 1: Changes of CD4+ T and CD8+ T cells during a period of seven years effective HAART (group A) ( ± SD, n = 25). *: P < 0.05 compared with Control, **: P < 0.05 compared with Pretherapy.

Mentions: Before treatment, the CD4+ T cell counts of group A patients were significantly lower than that in the control group (201 vs 751 cells/μL, p < 0.01) as shown in Figure 1. After seven years of effective HAART, CD4+ T cell counts had increased significantly (652 vs 201 cells/μL, p < 0.05). However, although the counts had reached a stable level after the third year of the treatment, it was still less than that in the control group after seven years effective HAART (652 vs 751 cells/μL, p < 0.05). The mean CD4+ T recovery in patients with 200-350/μL CD4+ T at baseline was higher than in patients starting with less than 200/μL CD4+ T. After 1, 3, 5 and 7 years of treatment the mean CD4+ T cell count in the 200-350/μL group increased to 605/μL, 775/μL, 801/μL and 838/ViL respectively. In the same time frame the mean CD4+ T cell count in the < 200/μL group increased to 328/μL, 476/μL, 485/μL and 498/μL respectively (Table 2).


Study of T cell subsets and IL-7 protein expression in HIV-1-infected patients after 7 years HAART.

Shou C, Weng N, Jin Y, Feng L, Jin C, Hoextermann S, Potthoff A, Skaletz-Rorowski A, Brockmeyer NH, Wu N - Eur. J. Med. Res. (2011)

Changes of CD4+ T and CD8+ T cells during a period of seven years effective HAART (group A) ( ± SD, n = 25). *: P < 0.05 compared with Control, **: P < 0.05 compared with Pretherapy.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3351803&req=5

Figure 1: Changes of CD4+ T and CD8+ T cells during a period of seven years effective HAART (group A) ( ± SD, n = 25). *: P < 0.05 compared with Control, **: P < 0.05 compared with Pretherapy.
Mentions: Before treatment, the CD4+ T cell counts of group A patients were significantly lower than that in the control group (201 vs 751 cells/μL, p < 0.01) as shown in Figure 1. After seven years of effective HAART, CD4+ T cell counts had increased significantly (652 vs 201 cells/μL, p < 0.05). However, although the counts had reached a stable level after the third year of the treatment, it was still less than that in the control group after seven years effective HAART (652 vs 751 cells/μL, p < 0.05). The mean CD4+ T recovery in patients with 200-350/μL CD4+ T at baseline was higher than in patients starting with less than 200/μL CD4+ T. After 1, 3, 5 and 7 years of treatment the mean CD4+ T cell count in the 200-350/μL group increased to 605/μL, 775/μL, 801/μL and 838/ViL respectively. In the same time frame the mean CD4+ T cell count in the < 200/μL group increased to 328/μL, 476/μL, 485/μL and 498/μL respectively (Table 2).

Bottom Line: At baseline CD4+ T cell counts of HIV-1-infected patients were lower than that in the control group (p < 0.01), whereas the CD8+, CD8/HLADR+ and CD8/CD38+ T cell counts were higher than those in the control group (p < 0.01).In the ineffective HAART group, the CD8/CD38+ T cell count had not decreased significantly, and CD8/HLADR+ T cell count gradually decreased.Before treatment, IL-7 serum levels of patients were significantly higher than that in the control group (p < 0.01).

View Article: PubMed Central - HTML - PubMed

Affiliation: Zhejiang University, School of Medicine, Hangzhou, China.

ABSTRACT

Objective: To study the changes in T cell subsets and IL-7 in HIV-1-infected patients after seven years of highly active antiretroviral therapy (HAART).

Methods: Seventy-five individuals were included in this study (25 with effective HAART, 18 with ineffective HAART, 17 untreated HIV+ patients, and 15 volunteers in the HIV negative control group). The counts of CD4+, CD8+, CD8/CD38+, and CD8/HLADR+ T cells as well as the IL-7 protein expression was measured at 5 time points during a period of seven years in patients starting HAART (baseline) and in the HIV negative control group. The expression of CD127 on CD3+ T cells was measured by flow cytometry at a single time point (after 7 years) in patients with HAART and was compared with untreated HIV+ patients and the HIV negative control group.

Results: At baseline CD4+ T cell counts of HIV-1-infected patients were lower than that in the control group (p < 0.01), whereas the CD8+, CD8/HLADR+ and CD8/CD38+ T cell counts were higher than those in the control group (p < 0.01). After seven years of effective HAART, the CD4+ T cell counts had increased and the CD8+ T cell count had decreased, although not to the normal levels (p < 0.05). Both the CD8/HLADR+ and CD8/CD38+ T cell counts had gradually approached those of the control group (p > 0.05). In the ineffective HAART group, the CD8/CD38+ T cell count had not decreased significantly, and CD8/HLADR+ T cell count gradually decreased. Before treatment, IL-7 serum levels of patients were significantly higher than that in the control group (p < 0.01). After seven years of effective HAART, IL-7 levels had gradually decreased, but were still higher than in the control group (p < 0.01). The CD127 expression on CD3+ CD8+ T cells in effective HAART patients was higher than in untreated HIV+ patients (p < 0.05), but was lower than that in the control group (p < 0.05). CD127 expression on CD3+ CD4+ T cells was not significantly different among the control group, untreated HIV+ patients and effective HAART group.

Conclusion: After seven years of effective HAART, the quantity and capacity of T cell subsets and IL-7 in HIV-1-infected patients had been partially restored, and the abnormal immune activation has significantly diminished.

Show MeSH
Related in: MedlinePlus