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Liposarcoma Cells with Aldefluor and CD133 Activity have a Cancer Stem Cell Potential.

Stratford EW, Castro R, Wennerstrom A, Holm R, Munthe E, Lauvrak S, Bjerkehagen B, Myklebost O - Clin Sarcoma Res (2011)

Bottom Line: Aldehyde dehydrogenase (ALDH) has recently been shown to be a marker of cancer stem-like cells (CSCs) across tumour types.Using a liposarcoma xenograft model, we have identified a small population of cells with an inducible stem cell potential, expressing both ALDH and CD133 following culturing in stem cell medium.This potential CSC population, which makes up for 0,1-1,7 % of the cells, displayed increased self-renewing abilities and increased tumourigenicity, giving tumours in vivo from as few as 100 injected cells.

View Article: PubMed Central - HTML - PubMed

ABSTRACT
Aldehyde dehydrogenase (ALDH) has recently been shown to be a marker of cancer stem-like cells (CSCs) across tumour types. The primary goals of this study were to investigate whether ALDH is expressed in liposarcomas, and whether CSCs can be identified in the ALDHhigh subpopulation. We have demonstrated that ALDH is indeed expressed in 10 out of 10 liposarcoma patient samples. Using a liposarcoma xenograft model, we have identified a small population of cells with an inducible stem cell potential, expressing both ALDH and CD133 following culturing in stem cell medium. This potential CSC population, which makes up for 0,1-1,7 % of the cells, displayed increased self-renewing abilities and increased tumourigenicity, giving tumours in vivo from as few as 100 injected cells.

No MeSH data available.


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Aldefluourhigh CD133high SW872 xenograft-derived cells differentiate into adipocytes. (A) Accumulation of lipid droplets indicative of mature adipocytes was observed following culturing of Aldefluourhigh CD133high sorted SW872 cells in medium supplemented with adipocytic differentiation cocktail (visualized by oil red O staining). (B) Aldefluourhigh CD133high sorted SW872 cells did not differentiate as efficiently when maintained in standard RPMI medium.
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Figure 4: Aldefluourhigh CD133high SW872 xenograft-derived cells differentiate into adipocytes. (A) Accumulation of lipid droplets indicative of mature adipocytes was observed following culturing of Aldefluourhigh CD133high sorted SW872 cells in medium supplemented with adipocytic differentiation cocktail (visualized by oil red O staining). (B) Aldefluourhigh CD133high sorted SW872 cells did not differentiate as efficiently when maintained in standard RPMI medium.

Mentions: According to the theory, a CSC has the ability to both generate more CSCs through self-renewal, and to undergo partial differentiation generating heterogeneous cancer cells, which make up the bulk of the tumour. Liposarcomas are in part composed of adipocytes and a potential liposarcoma CSC should therefore have the capacity to differentiate into adipocytes. When culturing the sorted cell populations in the presence of an adipocytic differentiation cocktail, we found that the Aldefluorhigh CD133high cells were able to differentiate into mature adipocytes more efficiently than the other sorted cell populations (Figure 4).


Liposarcoma Cells with Aldefluor and CD133 Activity have a Cancer Stem Cell Potential.

Stratford EW, Castro R, Wennerstrom A, Holm R, Munthe E, Lauvrak S, Bjerkehagen B, Myklebost O - Clin Sarcoma Res (2011)

Aldefluourhigh CD133high SW872 xenograft-derived cells differentiate into adipocytes. (A) Accumulation of lipid droplets indicative of mature adipocytes was observed following culturing of Aldefluourhigh CD133high sorted SW872 cells in medium supplemented with adipocytic differentiation cocktail (visualized by oil red O staining). (B) Aldefluourhigh CD133high sorted SW872 cells did not differentiate as efficiently when maintained in standard RPMI medium.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3351708&req=5

Figure 4: Aldefluourhigh CD133high SW872 xenograft-derived cells differentiate into adipocytes. (A) Accumulation of lipid droplets indicative of mature adipocytes was observed following culturing of Aldefluourhigh CD133high sorted SW872 cells in medium supplemented with adipocytic differentiation cocktail (visualized by oil red O staining). (B) Aldefluourhigh CD133high sorted SW872 cells did not differentiate as efficiently when maintained in standard RPMI medium.
Mentions: According to the theory, a CSC has the ability to both generate more CSCs through self-renewal, and to undergo partial differentiation generating heterogeneous cancer cells, which make up the bulk of the tumour. Liposarcomas are in part composed of adipocytes and a potential liposarcoma CSC should therefore have the capacity to differentiate into adipocytes. When culturing the sorted cell populations in the presence of an adipocytic differentiation cocktail, we found that the Aldefluorhigh CD133high cells were able to differentiate into mature adipocytes more efficiently than the other sorted cell populations (Figure 4).

Bottom Line: Aldehyde dehydrogenase (ALDH) has recently been shown to be a marker of cancer stem-like cells (CSCs) across tumour types.Using a liposarcoma xenograft model, we have identified a small population of cells with an inducible stem cell potential, expressing both ALDH and CD133 following culturing in stem cell medium.This potential CSC population, which makes up for 0,1-1,7 % of the cells, displayed increased self-renewing abilities and increased tumourigenicity, giving tumours in vivo from as few as 100 injected cells.

View Article: PubMed Central - HTML - PubMed

ABSTRACT
Aldehyde dehydrogenase (ALDH) has recently been shown to be a marker of cancer stem-like cells (CSCs) across tumour types. The primary goals of this study were to investigate whether ALDH is expressed in liposarcomas, and whether CSCs can be identified in the ALDHhigh subpopulation. We have demonstrated that ALDH is indeed expressed in 10 out of 10 liposarcoma patient samples. Using a liposarcoma xenograft model, we have identified a small population of cells with an inducible stem cell potential, expressing both ALDH and CD133 following culturing in stem cell medium. This potential CSC population, which makes up for 0,1-1,7 % of the cells, displayed increased self-renewing abilities and increased tumourigenicity, giving tumours in vivo from as few as 100 injected cells.

No MeSH data available.


Related in: MedlinePlus