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Liposarcoma Cells with Aldefluor and CD133 Activity have a Cancer Stem Cell Potential.

Stratford EW, Castro R, Wennerstrom A, Holm R, Munthe E, Lauvrak S, Bjerkehagen B, Myklebost O - Clin Sarcoma Res (2011)

Bottom Line: Aldehyde dehydrogenase (ALDH) has recently been shown to be a marker of cancer stem-like cells (CSCs) across tumour types.Using a liposarcoma xenograft model, we have identified a small population of cells with an inducible stem cell potential, expressing both ALDH and CD133 following culturing in stem cell medium.This potential CSC population, which makes up for 0,1-1,7 % of the cells, displayed increased self-renewing abilities and increased tumourigenicity, giving tumours in vivo from as few as 100 injected cells.

View Article: PubMed Central - HTML - PubMed

ABSTRACT
Aldehyde dehydrogenase (ALDH) has recently been shown to be a marker of cancer stem-like cells (CSCs) across tumour types. The primary goals of this study were to investigate whether ALDH is expressed in liposarcomas, and whether CSCs can be identified in the ALDHhigh subpopulation. We have demonstrated that ALDH is indeed expressed in 10 out of 10 liposarcoma patient samples. Using a liposarcoma xenograft model, we have identified a small population of cells with an inducible stem cell potential, expressing both ALDH and CD133 following culturing in stem cell medium. This potential CSC population, which makes up for 0,1-1,7 % of the cells, displayed increased self-renewing abilities and increased tumourigenicity, giving tumours in vivo from as few as 100 injected cells.

No MeSH data available.


Related in: MedlinePlus

Aldefluourhigh CD133high SW872 xenograft-derived cells form spheroids more effciently in soft agar. (A) Typical round-shaped spheroid of 50 μm formed from single Aldefluourhigh CD133high cell. (B) Aldefluorhigh CD133high cells formed spheroids with a frequency of up to 1 out of 4 cells (n = 4).
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Figure 3: Aldefluourhigh CD133high SW872 xenograft-derived cells form spheroids more effciently in soft agar. (A) Typical round-shaped spheroid of 50 μm formed from single Aldefluourhigh CD133high cell. (B) Aldefluorhigh CD133high cells formed spheroids with a frequency of up to 1 out of 4 cells (n = 4).

Mentions: Using flow cytometry, we isolated 4 subpopulations based on ALDH and CD133 expression. In order to investigate the different cell population's stem-like ability to self-renew, we performed spheroid assays in soft agar. The Aldefluorhigh CD133high cell population generated well-defined, round spheroids of approximately 50 μm in size (Figure 3A), at a frequency of up to 1 out of 4 cells. All the other three subpopulations generated spheroids at a significantly lower frequency (Figure 3B).


Liposarcoma Cells with Aldefluor and CD133 Activity have a Cancer Stem Cell Potential.

Stratford EW, Castro R, Wennerstrom A, Holm R, Munthe E, Lauvrak S, Bjerkehagen B, Myklebost O - Clin Sarcoma Res (2011)

Aldefluourhigh CD133high SW872 xenograft-derived cells form spheroids more effciently in soft agar. (A) Typical round-shaped spheroid of 50 μm formed from single Aldefluourhigh CD133high cell. (B) Aldefluorhigh CD133high cells formed spheroids with a frequency of up to 1 out of 4 cells (n = 4).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3351708&req=5

Figure 3: Aldefluourhigh CD133high SW872 xenograft-derived cells form spheroids more effciently in soft agar. (A) Typical round-shaped spheroid of 50 μm formed from single Aldefluourhigh CD133high cell. (B) Aldefluorhigh CD133high cells formed spheroids with a frequency of up to 1 out of 4 cells (n = 4).
Mentions: Using flow cytometry, we isolated 4 subpopulations based on ALDH and CD133 expression. In order to investigate the different cell population's stem-like ability to self-renew, we performed spheroid assays in soft agar. The Aldefluorhigh CD133high cell population generated well-defined, round spheroids of approximately 50 μm in size (Figure 3A), at a frequency of up to 1 out of 4 cells. All the other three subpopulations generated spheroids at a significantly lower frequency (Figure 3B).

Bottom Line: Aldehyde dehydrogenase (ALDH) has recently been shown to be a marker of cancer stem-like cells (CSCs) across tumour types.Using a liposarcoma xenograft model, we have identified a small population of cells with an inducible stem cell potential, expressing both ALDH and CD133 following culturing in stem cell medium.This potential CSC population, which makes up for 0,1-1,7 % of the cells, displayed increased self-renewing abilities and increased tumourigenicity, giving tumours in vivo from as few as 100 injected cells.

View Article: PubMed Central - HTML - PubMed

ABSTRACT
Aldehyde dehydrogenase (ALDH) has recently been shown to be a marker of cancer stem-like cells (CSCs) across tumour types. The primary goals of this study were to investigate whether ALDH is expressed in liposarcomas, and whether CSCs can be identified in the ALDHhigh subpopulation. We have demonstrated that ALDH is indeed expressed in 10 out of 10 liposarcoma patient samples. Using a liposarcoma xenograft model, we have identified a small population of cells with an inducible stem cell potential, expressing both ALDH and CD133 following culturing in stem cell medium. This potential CSC population, which makes up for 0,1-1,7 % of the cells, displayed increased self-renewing abilities and increased tumourigenicity, giving tumours in vivo from as few as 100 injected cells.

No MeSH data available.


Related in: MedlinePlus