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Liposarcoma Cells with Aldefluor and CD133 Activity have a Cancer Stem Cell Potential.

Stratford EW, Castro R, Wennerstrom A, Holm R, Munthe E, Lauvrak S, Bjerkehagen B, Myklebost O - Clin Sarcoma Res (2011)

Bottom Line: Aldehyde dehydrogenase (ALDH) has recently been shown to be a marker of cancer stem-like cells (CSCs) across tumour types.Using a liposarcoma xenograft model, we have identified a small population of cells with an inducible stem cell potential, expressing both ALDH and CD133 following culturing in stem cell medium.This potential CSC population, which makes up for 0,1-1,7 % of the cells, displayed increased self-renewing abilities and increased tumourigenicity, giving tumours in vivo from as few as 100 injected cells.

View Article: PubMed Central - HTML - PubMed

ABSTRACT
Aldehyde dehydrogenase (ALDH) has recently been shown to be a marker of cancer stem-like cells (CSCs) across tumour types. The primary goals of this study were to investigate whether ALDH is expressed in liposarcomas, and whether CSCs can be identified in the ALDHhigh subpopulation. We have demonstrated that ALDH is indeed expressed in 10 out of 10 liposarcoma patient samples. Using a liposarcoma xenograft model, we have identified a small population of cells with an inducible stem cell potential, expressing both ALDH and CD133 following culturing in stem cell medium. This potential CSC population, which makes up for 0,1-1,7 % of the cells, displayed increased self-renewing abilities and increased tumourigenicity, giving tumours in vivo from as few as 100 injected cells.

No MeSH data available.


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Characterisation of SW872 xenograft-derived cells following culturing in RPMI or stem cell medium. (A) Different morpholgy was observed dependent on the culturing medium. The cells appeared adherent when cultured in standard RPMI supplemented with fetal bovine serum (upper panel) and grew as detached spheroids when cultured in SC-medium (lower panel). (B) Flow diagrams are shown for control (DEAB) samples (left), and Aldefluor sample (right). 26% of the cells displayed Aldefluor activity when maintained in SC-medium (lower panel), compared to 13% of the cells when maintained in RPMI (upper panel). Aldefluor intensity is displayed along the X-axis. (C) Average Aldefluorhigh cells following culturing in SC-medium (35%) (black) (n = 10) or RPMI (11%) (grey) (n = 3).
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Figure 2: Characterisation of SW872 xenograft-derived cells following culturing in RPMI or stem cell medium. (A) Different morpholgy was observed dependent on the culturing medium. The cells appeared adherent when cultured in standard RPMI supplemented with fetal bovine serum (upper panel) and grew as detached spheroids when cultured in SC-medium (lower panel). (B) Flow diagrams are shown for control (DEAB) samples (left), and Aldefluor sample (right). 26% of the cells displayed Aldefluor activity when maintained in SC-medium (lower panel), compared to 13% of the cells when maintained in RPMI (upper panel). Aldefluor intensity is displayed along the X-axis. (C) Average Aldefluorhigh cells following culturing in SC-medium (35%) (black) (n = 10) or RPMI (11%) (grey) (n = 3).

Mentions: Having confirmed that ALDH1 is indeed expressed in human liposarcomas, we wanted to investigate whether liposarcoma ALDH-positive cells could be associated with CSC activity. We preferred to use a xenograft model, because the passing of the xenograft from mouse to mouse ensures that the growth conditions are physiological and that tumour initiating cells are present. Aldefluor analysis of cells extracted from the SW872 liposarcoma xenograft showed that the SW872 xenograft cells, like the liposarcoma patient samples, displayed ALDH activity (11% of the cells were Aldefluorhigh: Figure 2B), making xenograft-derived SW872 cells a suitable model for further analyses of ALDH-positive cells.


Liposarcoma Cells with Aldefluor and CD133 Activity have a Cancer Stem Cell Potential.

Stratford EW, Castro R, Wennerstrom A, Holm R, Munthe E, Lauvrak S, Bjerkehagen B, Myklebost O - Clin Sarcoma Res (2011)

Characterisation of SW872 xenograft-derived cells following culturing in RPMI or stem cell medium. (A) Different morpholgy was observed dependent on the culturing medium. The cells appeared adherent when cultured in standard RPMI supplemented with fetal bovine serum (upper panel) and grew as detached spheroids when cultured in SC-medium (lower panel). (B) Flow diagrams are shown for control (DEAB) samples (left), and Aldefluor sample (right). 26% of the cells displayed Aldefluor activity when maintained in SC-medium (lower panel), compared to 13% of the cells when maintained in RPMI (upper panel). Aldefluor intensity is displayed along the X-axis. (C) Average Aldefluorhigh cells following culturing in SC-medium (35%) (black) (n = 10) or RPMI (11%) (grey) (n = 3).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3351708&req=5

Figure 2: Characterisation of SW872 xenograft-derived cells following culturing in RPMI or stem cell medium. (A) Different morpholgy was observed dependent on the culturing medium. The cells appeared adherent when cultured in standard RPMI supplemented with fetal bovine serum (upper panel) and grew as detached spheroids when cultured in SC-medium (lower panel). (B) Flow diagrams are shown for control (DEAB) samples (left), and Aldefluor sample (right). 26% of the cells displayed Aldefluor activity when maintained in SC-medium (lower panel), compared to 13% of the cells when maintained in RPMI (upper panel). Aldefluor intensity is displayed along the X-axis. (C) Average Aldefluorhigh cells following culturing in SC-medium (35%) (black) (n = 10) or RPMI (11%) (grey) (n = 3).
Mentions: Having confirmed that ALDH1 is indeed expressed in human liposarcomas, we wanted to investigate whether liposarcoma ALDH-positive cells could be associated with CSC activity. We preferred to use a xenograft model, because the passing of the xenograft from mouse to mouse ensures that the growth conditions are physiological and that tumour initiating cells are present. Aldefluor analysis of cells extracted from the SW872 liposarcoma xenograft showed that the SW872 xenograft cells, like the liposarcoma patient samples, displayed ALDH activity (11% of the cells were Aldefluorhigh: Figure 2B), making xenograft-derived SW872 cells a suitable model for further analyses of ALDH-positive cells.

Bottom Line: Aldehyde dehydrogenase (ALDH) has recently been shown to be a marker of cancer stem-like cells (CSCs) across tumour types.Using a liposarcoma xenograft model, we have identified a small population of cells with an inducible stem cell potential, expressing both ALDH and CD133 following culturing in stem cell medium.This potential CSC population, which makes up for 0,1-1,7 % of the cells, displayed increased self-renewing abilities and increased tumourigenicity, giving tumours in vivo from as few as 100 injected cells.

View Article: PubMed Central - HTML - PubMed

ABSTRACT
Aldehyde dehydrogenase (ALDH) has recently been shown to be a marker of cancer stem-like cells (CSCs) across tumour types. The primary goals of this study were to investigate whether ALDH is expressed in liposarcomas, and whether CSCs can be identified in the ALDHhigh subpopulation. We have demonstrated that ALDH is indeed expressed in 10 out of 10 liposarcoma patient samples. Using a liposarcoma xenograft model, we have identified a small population of cells with an inducible stem cell potential, expressing both ALDH and CD133 following culturing in stem cell medium. This potential CSC population, which makes up for 0,1-1,7 % of the cells, displayed increased self-renewing abilities and increased tumourigenicity, giving tumours in vivo from as few as 100 injected cells.

No MeSH data available.


Related in: MedlinePlus