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NMR protocol for determination of oxidation susceptibility of serum lipids and application of the protocol to a chocolate study.

Tynkkynen T, Mursu J, Nurmi T, Tuppurainen K, Laatikainen R, Soininen P - Metabolomics (2011)

Bottom Line: The oxidation susceptibility of serum lipids decreased in the HPC group, and there was a significant difference between the WC and HPC groups (P = 0.031).Furthermore, arachidonic, docosahexaenoic, docosapentaenoic and palmitic acids, gamma-glutamyl transferase, hemoglobin, HDL, phosphatidylcholine and choline containing phospholipids explained about 60% of the oxidation susceptibility values.ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11306-011-0323-2) contains supplementary material, which is available to authorized users.

View Article: PubMed Central - PubMed

ABSTRACT
A protocol for determination of oxidation susceptibility of serum lipids based on proton nuclear magnetic resonance ((1)H NMR) spectroscopy is presented and compared to the commonly used spectrophotometric method. Even though there are methodological differences between these two methods, the NMR-based oxidation susceptibility correlates well (r(2) = 0.73) with the lag time determined spectrophotometrically. In addition to the oxidizability of serum lipids, the NMR method provides also information about the lipid profile. The NMR oxidation assay was applied to the chocolate study including fasting serum samples (n = 45) from subjects who had consumed white (WC), dark (DC) or high-polyphenol chocolate (HPC) daily for 3 weeks. The oxidation susceptibility of serum lipids decreased in the HPC group, and there was a significant difference between the WC and HPC groups (P = 0.031). According to the random forest analysis, the consumption of the HPC chocolate induced changes to the amounts of HDL, phosphatidylcholine, sphingomyelin, and nervonic, docosahexaenoic and myristic acids. Furthermore, arachidonic, docosahexaenoic, docosapentaenoic and palmitic acids, gamma-glutamyl transferase, hemoglobin, HDL, phosphatidylcholine and choline containing phospholipids explained about 60% of the oxidation susceptibility values. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11306-011-0323-2) contains supplementary material, which is available to authorized users.

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The amounts of oxidized PUFAs after the copper induced oxidation determined by the 1H NMR method plotted against the corresponding lag time values of the oxidation monitored spectrophotometrically from the chocolate study samples. The regression line is y = −0.2665x + 100.4, r2 = 0.7308
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Fig3: The amounts of oxidized PUFAs after the copper induced oxidation determined by the 1H NMR method plotted against the corresponding lag time values of the oxidation monitored spectrophotometrically from the chocolate study samples. The regression line is y = −0.2665x + 100.4, r2 = 0.7308

Mentions: The baseline and end-point oxidation susceptibility values determined by 1H NMR were plotted against the corresponding lag time values, and the strong correlation between these two methods (Spearman’s correlation coefficient −0.825, P < 0.001) is illustrated in Fig. 3. If a sample is very susceptible to oxidation, the lag time is short. By using the NMR method, this is seen as a high amount of oxidized PUFAs. However, even though the baseline and end-point values of the spectrophotometric and NMR method correlate, the change values (end-baseline), which are used in the ANOVA analysis, do not correlate (Pearson’s correlation coefficient −0.158, P = 0.317), and only the NMR oxidation assay reveals significant differences between the chocolate groups.Fig. 3


NMR protocol for determination of oxidation susceptibility of serum lipids and application of the protocol to a chocolate study.

Tynkkynen T, Mursu J, Nurmi T, Tuppurainen K, Laatikainen R, Soininen P - Metabolomics (2011)

The amounts of oxidized PUFAs after the copper induced oxidation determined by the 1H NMR method plotted against the corresponding lag time values of the oxidation monitored spectrophotometrically from the chocolate study samples. The regression line is y = −0.2665x + 100.4, r2 = 0.7308
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3351613&req=5

Fig3: The amounts of oxidized PUFAs after the copper induced oxidation determined by the 1H NMR method plotted against the corresponding lag time values of the oxidation monitored spectrophotometrically from the chocolate study samples. The regression line is y = −0.2665x + 100.4, r2 = 0.7308
Mentions: The baseline and end-point oxidation susceptibility values determined by 1H NMR were plotted against the corresponding lag time values, and the strong correlation between these two methods (Spearman’s correlation coefficient −0.825, P < 0.001) is illustrated in Fig. 3. If a sample is very susceptible to oxidation, the lag time is short. By using the NMR method, this is seen as a high amount of oxidized PUFAs. However, even though the baseline and end-point values of the spectrophotometric and NMR method correlate, the change values (end-baseline), which are used in the ANOVA analysis, do not correlate (Pearson’s correlation coefficient −0.158, P = 0.317), and only the NMR oxidation assay reveals significant differences between the chocolate groups.Fig. 3

Bottom Line: The oxidation susceptibility of serum lipids decreased in the HPC group, and there was a significant difference between the WC and HPC groups (P = 0.031).Furthermore, arachidonic, docosahexaenoic, docosapentaenoic and palmitic acids, gamma-glutamyl transferase, hemoglobin, HDL, phosphatidylcholine and choline containing phospholipids explained about 60% of the oxidation susceptibility values.ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11306-011-0323-2) contains supplementary material, which is available to authorized users.

View Article: PubMed Central - PubMed

ABSTRACT
A protocol for determination of oxidation susceptibility of serum lipids based on proton nuclear magnetic resonance ((1)H NMR) spectroscopy is presented and compared to the commonly used spectrophotometric method. Even though there are methodological differences between these two methods, the NMR-based oxidation susceptibility correlates well (r(2) = 0.73) with the lag time determined spectrophotometrically. In addition to the oxidizability of serum lipids, the NMR method provides also information about the lipid profile. The NMR oxidation assay was applied to the chocolate study including fasting serum samples (n = 45) from subjects who had consumed white (WC), dark (DC) or high-polyphenol chocolate (HPC) daily for 3 weeks. The oxidation susceptibility of serum lipids decreased in the HPC group, and there was a significant difference between the WC and HPC groups (P = 0.031). According to the random forest analysis, the consumption of the HPC chocolate induced changes to the amounts of HDL, phosphatidylcholine, sphingomyelin, and nervonic, docosahexaenoic and myristic acids. Furthermore, arachidonic, docosahexaenoic, docosapentaenoic and palmitic acids, gamma-glutamyl transferase, hemoglobin, HDL, phosphatidylcholine and choline containing phospholipids explained about 60% of the oxidation susceptibility values. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11306-011-0323-2) contains supplementary material, which is available to authorized users.

No MeSH data available.


Related in: MedlinePlus