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NMR protocol for determination of oxidation susceptibility of serum lipids and application of the protocol to a chocolate study.

Tynkkynen T, Mursu J, Nurmi T, Tuppurainen K, Laatikainen R, Soininen P - Metabolomics (2011)

Bottom Line: The oxidation susceptibility of serum lipids decreased in the HPC group, and there was a significant difference between the WC and HPC groups (P = 0.031).Furthermore, arachidonic, docosahexaenoic, docosapentaenoic and palmitic acids, gamma-glutamyl transferase, hemoglobin, HDL, phosphatidylcholine and choline containing phospholipids explained about 60% of the oxidation susceptibility values.ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11306-011-0323-2) contains supplementary material, which is available to authorized users.

View Article: PubMed Central - PubMed

ABSTRACT
A protocol for determination of oxidation susceptibility of serum lipids based on proton nuclear magnetic resonance ((1)H NMR) spectroscopy is presented and compared to the commonly used spectrophotometric method. Even though there are methodological differences between these two methods, the NMR-based oxidation susceptibility correlates well (r(2) = 0.73) with the lag time determined spectrophotometrically. In addition to the oxidizability of serum lipids, the NMR method provides also information about the lipid profile. The NMR oxidation assay was applied to the chocolate study including fasting serum samples (n = 45) from subjects who had consumed white (WC), dark (DC) or high-polyphenol chocolate (HPC) daily for 3 weeks. The oxidation susceptibility of serum lipids decreased in the HPC group, and there was a significant difference between the WC and HPC groups (P = 0.031). According to the random forest analysis, the consumption of the HPC chocolate induced changes to the amounts of HDL, phosphatidylcholine, sphingomyelin, and nervonic, docosahexaenoic and myristic acids. Furthermore, arachidonic, docosahexaenoic, docosapentaenoic and palmitic acids, gamma-glutamyl transferase, hemoglobin, HDL, phosphatidylcholine and choline containing phospholipids explained about 60% of the oxidation susceptibility values. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11306-011-0323-2) contains supplementary material, which is available to authorized users.

No MeSH data available.


The amounts of oxidized PUFAs during the copper induced oxidation with four different copper concentrations (a) and with a copper concentration of 0.5 mM for three subjects (b). Each data point in b is a mean of five determinations measured within period of 2 months
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Fig2: The amounts of oxidized PUFAs during the copper induced oxidation with four different copper concentrations (a) and with a copper concentration of 0.5 mM for three subjects (b). Each data point in b is a mean of five determinations measured within period of 2 months

Mentions: Several copper concentrations (0.5, 1.0, 2.0 and 4.0 mM) as well as incubation times (1.5, 3, 4.5 and 6 h) were tested in order to find out the copper concentration and the time point that yield maximal amount of information about the oxidation susceptibility of serum lipids as well as the largest variance between individuals. As can be seen from Fig. 2a, the oxidation reaction proceeds rapidly when copper concentrations of 1–4 mM are used. The copper concentration of 0.5 mM provides milder oxidation conditions and the oxidation proceeds slower, which also results in larger variance between individuals. The Fig. 2b shows that the largest variance between the percentage values describing the amount of oxidized PUFAs of the three subjects is obtained using incubation time of 6 hours. In addition, at this point, the speed of the oxidation reaction has slowed down allowing slight differences in the incubation times without affecting the results.Fig. 2


NMR protocol for determination of oxidation susceptibility of serum lipids and application of the protocol to a chocolate study.

Tynkkynen T, Mursu J, Nurmi T, Tuppurainen K, Laatikainen R, Soininen P - Metabolomics (2011)

The amounts of oxidized PUFAs during the copper induced oxidation with four different copper concentrations (a) and with a copper concentration of 0.5 mM for three subjects (b). Each data point in b is a mean of five determinations measured within period of 2 months
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3351613&req=5

Fig2: The amounts of oxidized PUFAs during the copper induced oxidation with four different copper concentrations (a) and with a copper concentration of 0.5 mM for three subjects (b). Each data point in b is a mean of five determinations measured within period of 2 months
Mentions: Several copper concentrations (0.5, 1.0, 2.0 and 4.0 mM) as well as incubation times (1.5, 3, 4.5 and 6 h) were tested in order to find out the copper concentration and the time point that yield maximal amount of information about the oxidation susceptibility of serum lipids as well as the largest variance between individuals. As can be seen from Fig. 2a, the oxidation reaction proceeds rapidly when copper concentrations of 1–4 mM are used. The copper concentration of 0.5 mM provides milder oxidation conditions and the oxidation proceeds slower, which also results in larger variance between individuals. The Fig. 2b shows that the largest variance between the percentage values describing the amount of oxidized PUFAs of the three subjects is obtained using incubation time of 6 hours. In addition, at this point, the speed of the oxidation reaction has slowed down allowing slight differences in the incubation times without affecting the results.Fig. 2

Bottom Line: The oxidation susceptibility of serum lipids decreased in the HPC group, and there was a significant difference between the WC and HPC groups (P = 0.031).Furthermore, arachidonic, docosahexaenoic, docosapentaenoic and palmitic acids, gamma-glutamyl transferase, hemoglobin, HDL, phosphatidylcholine and choline containing phospholipids explained about 60% of the oxidation susceptibility values.ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11306-011-0323-2) contains supplementary material, which is available to authorized users.

View Article: PubMed Central - PubMed

ABSTRACT
A protocol for determination of oxidation susceptibility of serum lipids based on proton nuclear magnetic resonance ((1)H NMR) spectroscopy is presented and compared to the commonly used spectrophotometric method. Even though there are methodological differences between these two methods, the NMR-based oxidation susceptibility correlates well (r(2) = 0.73) with the lag time determined spectrophotometrically. In addition to the oxidizability of serum lipids, the NMR method provides also information about the lipid profile. The NMR oxidation assay was applied to the chocolate study including fasting serum samples (n = 45) from subjects who had consumed white (WC), dark (DC) or high-polyphenol chocolate (HPC) daily for 3 weeks. The oxidation susceptibility of serum lipids decreased in the HPC group, and there was a significant difference between the WC and HPC groups (P = 0.031). According to the random forest analysis, the consumption of the HPC chocolate induced changes to the amounts of HDL, phosphatidylcholine, sphingomyelin, and nervonic, docosahexaenoic and myristic acids. Furthermore, arachidonic, docosahexaenoic, docosapentaenoic and palmitic acids, gamma-glutamyl transferase, hemoglobin, HDL, phosphatidylcholine and choline containing phospholipids explained about 60% of the oxidation susceptibility values. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11306-011-0323-2) contains supplementary material, which is available to authorized users.

No MeSH data available.