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Effects of dietary L-arginine or N-carbamylglutamate supplementation during late gestation of sows on the miR-15b/16, miR-221/222, VEGFA and eNOS expression in umbilical vein.

Liu XD, Wu X, Yin YL, Liu YQ, Geng MM, Yang HS, Blachier F, Wu GY - Amino Acids (2011)

Bottom Line: This study determined the effects of dietary arginine or N-carbamylglutamate (NCG) supplementation during late gestation of sows on the microRNAs, vascular endothelial growth factor A (VEGFA) and endothelial nitric oxide synthase (eNOS) expression in umbilical vein.The expression of VEGFA was higher (P<0.05) in the NCG-supplemented group than in the Arg-supplemented and the control group.In conclusion, this study demonstrated that dietary Arg or NCG supplementation may affect microRNAs (miR-15b, miR-222) targeting VEGFA and eNOS gene expressions in umbilical vein, so as to regulate the function and volume of the umbilical vein, provide more nutrients and oxygen from the maternal to the fetus tissue for fetal development and survival, and enhance the reproductive performance of sows.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Healthy Breeding of Livestock and Poultry, Hunan Engineering and Research Center of Animal and Poultry Science and Key Laboratory for Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, China.

ABSTRACT
Placental vascular formation and blood flow are crucial for fetal survival, growth and development, and arginine regulates vascular development and function. This study determined the effects of dietary arginine or N-carbamylglutamate (NCG) supplementation during late gestation of sows on the microRNAs, vascular endothelial growth factor A (VEGFA) and endothelial nitric oxide synthase (eNOS) expression in umbilical vein. Twenty-seven landrace×large white sows at day (d) 90 of gestation were assigned randomly to three groups and fed the following diets: a control diet and the control diet supplemented with 1.0% L-arginine or 0.10% NCG. Umbilical vein of fetuses with body weight around 2.0 kg (oversized), 1.5 kg (normal) and 0.6 kg (intrauterine growth restriction, IUGR) were obtained immediately after farrowing for miR-15b, miR-16, miR-221, miR-222, VEGFA and eNOS real-time PCR analysis. Compared with the control diets, dietary Arg or NCG supplementation enhanced the reproductive performance of sows, significantly increased (P<0.05) plasma arginine and decreased plasma VEGF and eNOS (P<0.05). The miR-15b expression in the umbilical vein was higher (P<0.05) in the NCG-supplemented group than in the control group. There was a trend in that the miR-222 expression in the umbilical vein of the oversized fetuses was higher (0.05

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Concentrations of free amino acids in plasma (μmol/L). For the same grayscale of the bar, values with different letter mean significant difference (P < 0.05), the same as given below
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Fig1: Concentrations of free amino acids in plasma (μmol/L). For the same grayscale of the bar, values with different letter mean significant difference (P < 0.05), the same as given below

Mentions: Concentrations of the most measured free amino acids in plasma did not differ among the three groups of sows at d 110 of gestation. Compared with the control group, dietary supplementation with Arg (P < 0.01) or NCG (P < 0.05) increased the concentrations of arginine in the plasma of sows (Fig. 1). Compared with the control diet and Arg-supplemented diet, NCG increased (P < 0.05) the concentrations of aspartate in the plasma of sows and decreased (P < 0.05) the concentrations of proline (Fig. 1).Fig. 1


Effects of dietary L-arginine or N-carbamylglutamate supplementation during late gestation of sows on the miR-15b/16, miR-221/222, VEGFA and eNOS expression in umbilical vein.

Liu XD, Wu X, Yin YL, Liu YQ, Geng MM, Yang HS, Blachier F, Wu GY - Amino Acids (2011)

Concentrations of free amino acids in plasma (μmol/L). For the same grayscale of the bar, values with different letter mean significant difference (P < 0.05), the same as given below
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3351605&req=5

Fig1: Concentrations of free amino acids in plasma (μmol/L). For the same grayscale of the bar, values with different letter mean significant difference (P < 0.05), the same as given below
Mentions: Concentrations of the most measured free amino acids in plasma did not differ among the three groups of sows at d 110 of gestation. Compared with the control group, dietary supplementation with Arg (P < 0.01) or NCG (P < 0.05) increased the concentrations of arginine in the plasma of sows (Fig. 1). Compared with the control diet and Arg-supplemented diet, NCG increased (P < 0.05) the concentrations of aspartate in the plasma of sows and decreased (P < 0.05) the concentrations of proline (Fig. 1).Fig. 1

Bottom Line: This study determined the effects of dietary arginine or N-carbamylglutamate (NCG) supplementation during late gestation of sows on the microRNAs, vascular endothelial growth factor A (VEGFA) and endothelial nitric oxide synthase (eNOS) expression in umbilical vein.The expression of VEGFA was higher (P<0.05) in the NCG-supplemented group than in the Arg-supplemented and the control group.In conclusion, this study demonstrated that dietary Arg or NCG supplementation may affect microRNAs (miR-15b, miR-222) targeting VEGFA and eNOS gene expressions in umbilical vein, so as to regulate the function and volume of the umbilical vein, provide more nutrients and oxygen from the maternal to the fetus tissue for fetal development and survival, and enhance the reproductive performance of sows.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Healthy Breeding of Livestock and Poultry, Hunan Engineering and Research Center of Animal and Poultry Science and Key Laboratory for Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, China.

ABSTRACT
Placental vascular formation and blood flow are crucial for fetal survival, growth and development, and arginine regulates vascular development and function. This study determined the effects of dietary arginine or N-carbamylglutamate (NCG) supplementation during late gestation of sows on the microRNAs, vascular endothelial growth factor A (VEGFA) and endothelial nitric oxide synthase (eNOS) expression in umbilical vein. Twenty-seven landrace×large white sows at day (d) 90 of gestation were assigned randomly to three groups and fed the following diets: a control diet and the control diet supplemented with 1.0% L-arginine or 0.10% NCG. Umbilical vein of fetuses with body weight around 2.0 kg (oversized), 1.5 kg (normal) and 0.6 kg (intrauterine growth restriction, IUGR) were obtained immediately after farrowing for miR-15b, miR-16, miR-221, miR-222, VEGFA and eNOS real-time PCR analysis. Compared with the control diets, dietary Arg or NCG supplementation enhanced the reproductive performance of sows, significantly increased (P<0.05) plasma arginine and decreased plasma VEGF and eNOS (P<0.05). The miR-15b expression in the umbilical vein was higher (P<0.05) in the NCG-supplemented group than in the control group. There was a trend in that the miR-222 expression in the umbilical vein of the oversized fetuses was higher (0.05

Show MeSH
Related in: MedlinePlus