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Regular exercise or changing diet does not influence aortic valve disease progression in LDLR deficient mice.

Schlotter F, Matsumoto Y, Mangner N, Schuler G, Linke A, Adams V - PLoS ONE (2012)

Bottom Line: Histological analysis of the aortic valve thickness demonstrated no significant difference between the three groups (control 98.3±4.5 µm, ET 88.2±6.6 µm, change in diet 87.5±4.0).Furthermore no difference between the groups was evident with respect to the expression of inflammatory, fibroblastic and osteoblastic markers.Based on the present study we have to conclude that once the development of a CAVD is initiated, exercise training or a change in diet does not have the potential to attenuate the progress of the CAVD.

View Article: PubMed Central - PubMed

Affiliation: University of Leipzig-Heart Center Leipzig, Department of Internal Medicine/Cardiology, Leipzig, Germany.

ABSTRACT

Background: The development and progression of calcific aortic valve disease (CAVD) shares a number of similarities with atherosclerosis. Recently we could demonstrate that regular exercise training (ET) as primary prevention prevents aortic valve disease in LDL-receptor deficient (LDLR(-/-)) mice. We aimed to investigate the impact of exercise training on the progression of CAVD in LDLR(-/-) mice in the setting of secondary prevention

Methods and results: Sixty-four LDLR(-/-) mice were fed with high cholesterol diet to induce aortic valve sclerosis. Thereafter the animals were divided into 3 groups: group 1 continuing on high cholesterol diet, group 2 continuing with cholesterol diet plus 1 h ET per day, group 3 continuing with normal mouse chow. After another 16 weeks the animal were sacrificed. Histological analysis of the aortic valve thickness demonstrated no significant difference between the three groups (control 98.3±4.5 µm, ET 88.2±6.6 µm, change in diet 87.5±4.0). Immunohistochemical staining for endothelial cells revealed a disrupted endothelial cell layer to the same extend in all groups. Furthermore no difference between the groups was evident with respect to the expression of inflammatory, fibroblastic and osteoblastic markers.

Conclusion: Based on the present study we have to conclude that once the development of a CAVD is initiated, exercise training or a change in diet does not have the potential to attenuate the progress of the CAVD.

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Representative immunostaining for angiotensin II receptor type 1 (AT1) in the control group, the cholesterol diet plus exercise training group and the normal diet plus sedentary activity (A–C).Semi-quantitative analysis of AT1-positive cells in 8 animals from each group (D). Arrowheads indicate positive staining of the endothelium serving as internal positive control. Bar = 100 µm.
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pone-0037298-g006: Representative immunostaining for angiotensin II receptor type 1 (AT1) in the control group, the cholesterol diet plus exercise training group and the normal diet plus sedentary activity (A–C).Semi-quantitative analysis of AT1-positive cells in 8 animals from each group (D). Arrowheads indicate positive staining of the endothelium serving as internal positive control. Bar = 100 µm.

Mentions: The angiotensin II receptor type 1 (AT1), described as a further element of the pathologic changes inside the AV leaflets, was markedly down-regulated in its expression in those animals receiving exercise training. Semi-quantitative analysis showed a significantly lower expression of AT1 in the exercise group, while AVs of the control group and change of diet group exhibited high degrees of expression of AT1 (Figure 6).


Regular exercise or changing diet does not influence aortic valve disease progression in LDLR deficient mice.

Schlotter F, Matsumoto Y, Mangner N, Schuler G, Linke A, Adams V - PLoS ONE (2012)

Representative immunostaining for angiotensin II receptor type 1 (AT1) in the control group, the cholesterol diet plus exercise training group and the normal diet plus sedentary activity (A–C).Semi-quantitative analysis of AT1-positive cells in 8 animals from each group (D). Arrowheads indicate positive staining of the endothelium serving as internal positive control. Bar = 100 µm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3351435&req=5

pone-0037298-g006: Representative immunostaining for angiotensin II receptor type 1 (AT1) in the control group, the cholesterol diet plus exercise training group and the normal diet plus sedentary activity (A–C).Semi-quantitative analysis of AT1-positive cells in 8 animals from each group (D). Arrowheads indicate positive staining of the endothelium serving as internal positive control. Bar = 100 µm.
Mentions: The angiotensin II receptor type 1 (AT1), described as a further element of the pathologic changes inside the AV leaflets, was markedly down-regulated in its expression in those animals receiving exercise training. Semi-quantitative analysis showed a significantly lower expression of AT1 in the exercise group, while AVs of the control group and change of diet group exhibited high degrees of expression of AT1 (Figure 6).

Bottom Line: Histological analysis of the aortic valve thickness demonstrated no significant difference between the three groups (control 98.3±4.5 µm, ET 88.2±6.6 µm, change in diet 87.5±4.0).Furthermore no difference between the groups was evident with respect to the expression of inflammatory, fibroblastic and osteoblastic markers.Based on the present study we have to conclude that once the development of a CAVD is initiated, exercise training or a change in diet does not have the potential to attenuate the progress of the CAVD.

View Article: PubMed Central - PubMed

Affiliation: University of Leipzig-Heart Center Leipzig, Department of Internal Medicine/Cardiology, Leipzig, Germany.

ABSTRACT

Background: The development and progression of calcific aortic valve disease (CAVD) shares a number of similarities with atherosclerosis. Recently we could demonstrate that regular exercise training (ET) as primary prevention prevents aortic valve disease in LDL-receptor deficient (LDLR(-/-)) mice. We aimed to investigate the impact of exercise training on the progression of CAVD in LDLR(-/-) mice in the setting of secondary prevention

Methods and results: Sixty-four LDLR(-/-) mice were fed with high cholesterol diet to induce aortic valve sclerosis. Thereafter the animals were divided into 3 groups: group 1 continuing on high cholesterol diet, group 2 continuing with cholesterol diet plus 1 h ET per day, group 3 continuing with normal mouse chow. After another 16 weeks the animal were sacrificed. Histological analysis of the aortic valve thickness demonstrated no significant difference between the three groups (control 98.3±4.5 µm, ET 88.2±6.6 µm, change in diet 87.5±4.0). Immunohistochemical staining for endothelial cells revealed a disrupted endothelial cell layer to the same extend in all groups. Furthermore no difference between the groups was evident with respect to the expression of inflammatory, fibroblastic and osteoblastic markers.

Conclusion: Based on the present study we have to conclude that once the development of a CAVD is initiated, exercise training or a change in diet does not have the potential to attenuate the progress of the CAVD.

Show MeSH
Related in: MedlinePlus