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Regular exercise or changing diet does not influence aortic valve disease progression in LDLR deficient mice.

Schlotter F, Matsumoto Y, Mangner N, Schuler G, Linke A, Adams V - PLoS ONE (2012)

Bottom Line: Histological analysis of the aortic valve thickness demonstrated no significant difference between the three groups (control 98.3±4.5 µm, ET 88.2±6.6 µm, change in diet 87.5±4.0).Furthermore no difference between the groups was evident with respect to the expression of inflammatory, fibroblastic and osteoblastic markers.Based on the present study we have to conclude that once the development of a CAVD is initiated, exercise training or a change in diet does not have the potential to attenuate the progress of the CAVD.

View Article: PubMed Central - PubMed

Affiliation: University of Leipzig-Heart Center Leipzig, Department of Internal Medicine/Cardiology, Leipzig, Germany.

ABSTRACT

Background: The development and progression of calcific aortic valve disease (CAVD) shares a number of similarities with atherosclerosis. Recently we could demonstrate that regular exercise training (ET) as primary prevention prevents aortic valve disease in LDL-receptor deficient (LDLR(-/-)) mice. We aimed to investigate the impact of exercise training on the progression of CAVD in LDLR(-/-) mice in the setting of secondary prevention

Methods and results: Sixty-four LDLR(-/-) mice were fed with high cholesterol diet to induce aortic valve sclerosis. Thereafter the animals were divided into 3 groups: group 1 continuing on high cholesterol diet, group 2 continuing with cholesterol diet plus 1 h ET per day, group 3 continuing with normal mouse chow. After another 16 weeks the animal were sacrificed. Histological analysis of the aortic valve thickness demonstrated no significant difference between the three groups (control 98.3±4.5 µm, ET 88.2±6.6 µm, change in diet 87.5±4.0). Immunohistochemical staining for endothelial cells revealed a disrupted endothelial cell layer to the same extend in all groups. Furthermore no difference between the groups was evident with respect to the expression of inflammatory, fibroblastic and osteoblastic markers.

Conclusion: Based on the present study we have to conclude that once the development of a CAVD is initiated, exercise training or a change in diet does not have the potential to attenuate the progress of the CAVD.

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Quantitative analysis of messenger RNA expression for: α–smooth muscle actin, bone morphogenetic protein-2 (BMP-2, a mediator of calcification) (B) from aortic valve cusps of 8 animals from each group.arb. Units = arbitrary units. An example of a von Kossa staining is shown (C) (mineralization is shown as black dots).
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pone-0037298-g005: Quantitative analysis of messenger RNA expression for: α–smooth muscle actin, bone morphogenetic protein-2 (BMP-2, a mediator of calcification) (B) from aortic valve cusps of 8 animals from each group.arb. Units = arbitrary units. An example of a von Kossa staining is shown (C) (mineralization is shown as black dots).

Mentions: α-smooth muscle actin-positive myofibroblasts were abundant inside the AVs of all three study groups. All AV leaflets showed a high level of α-smooth muscle actin-positive cells demonstrating fibrotic transformation. Semi-quantitative analysis revealed no significant differences between the 3 groups (Figure 4). Analysis of the m-RNA expression of α-smooth muscle actin confirmed the results of the immunohistochemical analyses with no significant differences observable between all study groups (control: 2.6±0.3 arb. units, exercise: 3.3±0.6 arb. units, change of diet: 3.1±1.3 arb. units; p = n.s.) (Figure 5A).


Regular exercise or changing diet does not influence aortic valve disease progression in LDLR deficient mice.

Schlotter F, Matsumoto Y, Mangner N, Schuler G, Linke A, Adams V - PLoS ONE (2012)

Quantitative analysis of messenger RNA expression for: α–smooth muscle actin, bone morphogenetic protein-2 (BMP-2, a mediator of calcification) (B) from aortic valve cusps of 8 animals from each group.arb. Units = arbitrary units. An example of a von Kossa staining is shown (C) (mineralization is shown as black dots).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3351435&req=5

pone-0037298-g005: Quantitative analysis of messenger RNA expression for: α–smooth muscle actin, bone morphogenetic protein-2 (BMP-2, a mediator of calcification) (B) from aortic valve cusps of 8 animals from each group.arb. Units = arbitrary units. An example of a von Kossa staining is shown (C) (mineralization is shown as black dots).
Mentions: α-smooth muscle actin-positive myofibroblasts were abundant inside the AVs of all three study groups. All AV leaflets showed a high level of α-smooth muscle actin-positive cells demonstrating fibrotic transformation. Semi-quantitative analysis revealed no significant differences between the 3 groups (Figure 4). Analysis of the m-RNA expression of α-smooth muscle actin confirmed the results of the immunohistochemical analyses with no significant differences observable between all study groups (control: 2.6±0.3 arb. units, exercise: 3.3±0.6 arb. units, change of diet: 3.1±1.3 arb. units; p = n.s.) (Figure 5A).

Bottom Line: Histological analysis of the aortic valve thickness demonstrated no significant difference between the three groups (control 98.3±4.5 µm, ET 88.2±6.6 µm, change in diet 87.5±4.0).Furthermore no difference between the groups was evident with respect to the expression of inflammatory, fibroblastic and osteoblastic markers.Based on the present study we have to conclude that once the development of a CAVD is initiated, exercise training or a change in diet does not have the potential to attenuate the progress of the CAVD.

View Article: PubMed Central - PubMed

Affiliation: University of Leipzig-Heart Center Leipzig, Department of Internal Medicine/Cardiology, Leipzig, Germany.

ABSTRACT

Background: The development and progression of calcific aortic valve disease (CAVD) shares a number of similarities with atherosclerosis. Recently we could demonstrate that regular exercise training (ET) as primary prevention prevents aortic valve disease in LDL-receptor deficient (LDLR(-/-)) mice. We aimed to investigate the impact of exercise training on the progression of CAVD in LDLR(-/-) mice in the setting of secondary prevention

Methods and results: Sixty-four LDLR(-/-) mice were fed with high cholesterol diet to induce aortic valve sclerosis. Thereafter the animals were divided into 3 groups: group 1 continuing on high cholesterol diet, group 2 continuing with cholesterol diet plus 1 h ET per day, group 3 continuing with normal mouse chow. After another 16 weeks the animal were sacrificed. Histological analysis of the aortic valve thickness demonstrated no significant difference between the three groups (control 98.3±4.5 µm, ET 88.2±6.6 µm, change in diet 87.5±4.0). Immunohistochemical staining for endothelial cells revealed a disrupted endothelial cell layer to the same extend in all groups. Furthermore no difference between the groups was evident with respect to the expression of inflammatory, fibroblastic and osteoblastic markers.

Conclusion: Based on the present study we have to conclude that once the development of a CAVD is initiated, exercise training or a change in diet does not have the potential to attenuate the progress of the CAVD.

Show MeSH
Related in: MedlinePlus