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Regular exercise or changing diet does not influence aortic valve disease progression in LDLR deficient mice.

Schlotter F, Matsumoto Y, Mangner N, Schuler G, Linke A, Adams V - PLoS ONE (2012)

Bottom Line: Histological analysis of the aortic valve thickness demonstrated no significant difference between the three groups (control 98.3±4.5 µm, ET 88.2±6.6 µm, change in diet 87.5±4.0).Furthermore no difference between the groups was evident with respect to the expression of inflammatory, fibroblastic and osteoblastic markers.Based on the present study we have to conclude that once the development of a CAVD is initiated, exercise training or a change in diet does not have the potential to attenuate the progress of the CAVD.

View Article: PubMed Central - PubMed

Affiliation: University of Leipzig-Heart Center Leipzig, Department of Internal Medicine/Cardiology, Leipzig, Germany.

ABSTRACT

Background: The development and progression of calcific aortic valve disease (CAVD) shares a number of similarities with atherosclerosis. Recently we could demonstrate that regular exercise training (ET) as primary prevention prevents aortic valve disease in LDL-receptor deficient (LDLR(-/-)) mice. We aimed to investigate the impact of exercise training on the progression of CAVD in LDLR(-/-) mice in the setting of secondary prevention

Methods and results: Sixty-four LDLR(-/-) mice were fed with high cholesterol diet to induce aortic valve sclerosis. Thereafter the animals were divided into 3 groups: group 1 continuing on high cholesterol diet, group 2 continuing with cholesterol diet plus 1 h ET per day, group 3 continuing with normal mouse chow. After another 16 weeks the animal were sacrificed. Histological analysis of the aortic valve thickness demonstrated no significant difference between the three groups (control 98.3±4.5 µm, ET 88.2±6.6 µm, change in diet 87.5±4.0). Immunohistochemical staining for endothelial cells revealed a disrupted endothelial cell layer to the same extend in all groups. Furthermore no difference between the groups was evident with respect to the expression of inflammatory, fibroblastic and osteoblastic markers.

Conclusion: Based on the present study we have to conclude that once the development of a CAVD is initiated, exercise training or a change in diet does not have the potential to attenuate the progress of the CAVD.

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Representative immunostaining for endothelial nitric oxide synthase (eNOS) in all three animal groups.(A) Cholesterol diet plus sedentary activity (control group); (B) cholesterol diet plus exercise training; (C) normal diet plus sedentary activity. Quantitative analysis of eNOS-positive circumference in 9 animals per group (D). Arrowheads indicate disrupted area of eNOS expression. Bar = 100 µm.
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pone-0037298-g003: Representative immunostaining for endothelial nitric oxide synthase (eNOS) in all three animal groups.(A) Cholesterol diet plus sedentary activity (control group); (B) cholesterol diet plus exercise training; (C) normal diet plus sedentary activity. Quantitative analysis of eNOS-positive circumference in 9 animals per group (D). Arrowheads indicate disrupted area of eNOS expression. Bar = 100 µm.

Mentions: In the control animals 62.6±5.4% of the AV surface was covered with endothelium as evident by eNOS-positive stained cells. An exercise training program showed no significant impact on the degree of endothelial cell coverage (exercise group 66.2±4.3%). The cholesterol-diet induced disruption of the endothelial layer could also not be preserved significantly by a change of diet (change in diet group 72.7±3.4%) (Figure 3).


Regular exercise or changing diet does not influence aortic valve disease progression in LDLR deficient mice.

Schlotter F, Matsumoto Y, Mangner N, Schuler G, Linke A, Adams V - PLoS ONE (2012)

Representative immunostaining for endothelial nitric oxide synthase (eNOS) in all three animal groups.(A) Cholesterol diet plus sedentary activity (control group); (B) cholesterol diet plus exercise training; (C) normal diet plus sedentary activity. Quantitative analysis of eNOS-positive circumference in 9 animals per group (D). Arrowheads indicate disrupted area of eNOS expression. Bar = 100 µm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3351435&req=5

pone-0037298-g003: Representative immunostaining for endothelial nitric oxide synthase (eNOS) in all three animal groups.(A) Cholesterol diet plus sedentary activity (control group); (B) cholesterol diet plus exercise training; (C) normal diet plus sedentary activity. Quantitative analysis of eNOS-positive circumference in 9 animals per group (D). Arrowheads indicate disrupted area of eNOS expression. Bar = 100 µm.
Mentions: In the control animals 62.6±5.4% of the AV surface was covered with endothelium as evident by eNOS-positive stained cells. An exercise training program showed no significant impact on the degree of endothelial cell coverage (exercise group 66.2±4.3%). The cholesterol-diet induced disruption of the endothelial layer could also not be preserved significantly by a change of diet (change in diet group 72.7±3.4%) (Figure 3).

Bottom Line: Histological analysis of the aortic valve thickness demonstrated no significant difference between the three groups (control 98.3±4.5 µm, ET 88.2±6.6 µm, change in diet 87.5±4.0).Furthermore no difference between the groups was evident with respect to the expression of inflammatory, fibroblastic and osteoblastic markers.Based on the present study we have to conclude that once the development of a CAVD is initiated, exercise training or a change in diet does not have the potential to attenuate the progress of the CAVD.

View Article: PubMed Central - PubMed

Affiliation: University of Leipzig-Heart Center Leipzig, Department of Internal Medicine/Cardiology, Leipzig, Germany.

ABSTRACT

Background: The development and progression of calcific aortic valve disease (CAVD) shares a number of similarities with atherosclerosis. Recently we could demonstrate that regular exercise training (ET) as primary prevention prevents aortic valve disease in LDL-receptor deficient (LDLR(-/-)) mice. We aimed to investigate the impact of exercise training on the progression of CAVD in LDLR(-/-) mice in the setting of secondary prevention

Methods and results: Sixty-four LDLR(-/-) mice were fed with high cholesterol diet to induce aortic valve sclerosis. Thereafter the animals were divided into 3 groups: group 1 continuing on high cholesterol diet, group 2 continuing with cholesterol diet plus 1 h ET per day, group 3 continuing with normal mouse chow. After another 16 weeks the animal were sacrificed. Histological analysis of the aortic valve thickness demonstrated no significant difference between the three groups (control 98.3±4.5 µm, ET 88.2±6.6 µm, change in diet 87.5±4.0). Immunohistochemical staining for endothelial cells revealed a disrupted endothelial cell layer to the same extend in all groups. Furthermore no difference between the groups was evident with respect to the expression of inflammatory, fibroblastic and osteoblastic markers.

Conclusion: Based on the present study we have to conclude that once the development of a CAVD is initiated, exercise training or a change in diet does not have the potential to attenuate the progress of the CAVD.

Show MeSH
Related in: MedlinePlus