Limits...
Regular exercise or changing diet does not influence aortic valve disease progression in LDLR deficient mice.

Schlotter F, Matsumoto Y, Mangner N, Schuler G, Linke A, Adams V - PLoS ONE (2012)

Bottom Line: Histological analysis of the aortic valve thickness demonstrated no significant difference between the three groups (control 98.3±4.5 µm, ET 88.2±6.6 µm, change in diet 87.5±4.0).Furthermore no difference between the groups was evident with respect to the expression of inflammatory, fibroblastic and osteoblastic markers.Based on the present study we have to conclude that once the development of a CAVD is initiated, exercise training or a change in diet does not have the potential to attenuate the progress of the CAVD.

View Article: PubMed Central - PubMed

Affiliation: University of Leipzig-Heart Center Leipzig, Department of Internal Medicine/Cardiology, Leipzig, Germany.

ABSTRACT

Background: The development and progression of calcific aortic valve disease (CAVD) shares a number of similarities with atherosclerosis. Recently we could demonstrate that regular exercise training (ET) as primary prevention prevents aortic valve disease in LDL-receptor deficient (LDLR(-/-)) mice. We aimed to investigate the impact of exercise training on the progression of CAVD in LDLR(-/-) mice in the setting of secondary prevention

Methods and results: Sixty-four LDLR(-/-) mice were fed with high cholesterol diet to induce aortic valve sclerosis. Thereafter the animals were divided into 3 groups: group 1 continuing on high cholesterol diet, group 2 continuing with cholesterol diet plus 1 h ET per day, group 3 continuing with normal mouse chow. After another 16 weeks the animal were sacrificed. Histological analysis of the aortic valve thickness demonstrated no significant difference between the three groups (control 98.3±4.5 µm, ET 88.2±6.6 µm, change in diet 87.5±4.0). Immunohistochemical staining for endothelial cells revealed a disrupted endothelial cell layer to the same extend in all groups. Furthermore no difference between the groups was evident with respect to the expression of inflammatory, fibroblastic and osteoblastic markers.

Conclusion: Based on the present study we have to conclude that once the development of a CAVD is initiated, exercise training or a change in diet does not have the potential to attenuate the progress of the CAVD.

Show MeSH

Related in: MedlinePlus

Experimental study design.Four-week old LDLR−/− mice were fed cholesterol diet for 16 weeks. At week 20 all animals were randomized into one of the following groups: Group 1 -control group- (cholesterol diet plus sedentary activity); group 2 –exercise training group- (cholesterol diet plus exercise training); group 3 -change of diet group- (normal diet plus sedentary activity).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3351435&req=5

pone-0037298-g001: Experimental study design.Four-week old LDLR−/− mice were fed cholesterol diet for 16 weeks. At week 20 all animals were randomized into one of the following groups: Group 1 -control group- (cholesterol diet plus sedentary activity); group 2 –exercise training group- (cholesterol diet plus exercise training); group 3 -change of diet group- (normal diet plus sedentary activity).

Mentions: A total of 64 low-density lipoprotein (LDL)-receptor-deficient (LDLR−/−) mice on the C57BL/6J background at the age of 4 weeks were fed with cholesterol rich diet until 20 weeks. At 20 weeks, the mice were randomly divided into 3 groups: group 1 (control group), cholesterol diet plus sedentary lifestyle, group 2 (exercise group), cholesterol diet plus exercise training (ET) (1 h/day, 5 days/week), and group 3 (change of diet group), normal diet plus sedentary lifestyle. At an age of 36 weeks the animals were sacrificed by cervical dislocation and the tissue material was harvested for further analyses (Figure 1).


Regular exercise or changing diet does not influence aortic valve disease progression in LDLR deficient mice.

Schlotter F, Matsumoto Y, Mangner N, Schuler G, Linke A, Adams V - PLoS ONE (2012)

Experimental study design.Four-week old LDLR−/− mice were fed cholesterol diet for 16 weeks. At week 20 all animals were randomized into one of the following groups: Group 1 -control group- (cholesterol diet plus sedentary activity); group 2 –exercise training group- (cholesterol diet plus exercise training); group 3 -change of diet group- (normal diet plus sedentary activity).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3351435&req=5

pone-0037298-g001: Experimental study design.Four-week old LDLR−/− mice were fed cholesterol diet for 16 weeks. At week 20 all animals were randomized into one of the following groups: Group 1 -control group- (cholesterol diet plus sedentary activity); group 2 –exercise training group- (cholesterol diet plus exercise training); group 3 -change of diet group- (normal diet plus sedentary activity).
Mentions: A total of 64 low-density lipoprotein (LDL)-receptor-deficient (LDLR−/−) mice on the C57BL/6J background at the age of 4 weeks were fed with cholesterol rich diet until 20 weeks. At 20 weeks, the mice were randomly divided into 3 groups: group 1 (control group), cholesterol diet plus sedentary lifestyle, group 2 (exercise group), cholesterol diet plus exercise training (ET) (1 h/day, 5 days/week), and group 3 (change of diet group), normal diet plus sedentary lifestyle. At an age of 36 weeks the animals were sacrificed by cervical dislocation and the tissue material was harvested for further analyses (Figure 1).

Bottom Line: Histological analysis of the aortic valve thickness demonstrated no significant difference between the three groups (control 98.3±4.5 µm, ET 88.2±6.6 µm, change in diet 87.5±4.0).Furthermore no difference between the groups was evident with respect to the expression of inflammatory, fibroblastic and osteoblastic markers.Based on the present study we have to conclude that once the development of a CAVD is initiated, exercise training or a change in diet does not have the potential to attenuate the progress of the CAVD.

View Article: PubMed Central - PubMed

Affiliation: University of Leipzig-Heart Center Leipzig, Department of Internal Medicine/Cardiology, Leipzig, Germany.

ABSTRACT

Background: The development and progression of calcific aortic valve disease (CAVD) shares a number of similarities with atherosclerosis. Recently we could demonstrate that regular exercise training (ET) as primary prevention prevents aortic valve disease in LDL-receptor deficient (LDLR(-/-)) mice. We aimed to investigate the impact of exercise training on the progression of CAVD in LDLR(-/-) mice in the setting of secondary prevention

Methods and results: Sixty-four LDLR(-/-) mice were fed with high cholesterol diet to induce aortic valve sclerosis. Thereafter the animals were divided into 3 groups: group 1 continuing on high cholesterol diet, group 2 continuing with cholesterol diet plus 1 h ET per day, group 3 continuing with normal mouse chow. After another 16 weeks the animal were sacrificed. Histological analysis of the aortic valve thickness demonstrated no significant difference between the three groups (control 98.3±4.5 µm, ET 88.2±6.6 µm, change in diet 87.5±4.0). Immunohistochemical staining for endothelial cells revealed a disrupted endothelial cell layer to the same extend in all groups. Furthermore no difference between the groups was evident with respect to the expression of inflammatory, fibroblastic and osteoblastic markers.

Conclusion: Based on the present study we have to conclude that once the development of a CAVD is initiated, exercise training or a change in diet does not have the potential to attenuate the progress of the CAVD.

Show MeSH
Related in: MedlinePlus