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Hyaluronic acid increases MMP-2 and MMP-9 expressions in cultured trabecular meshwork cells from patients with primary open-angle glaucoma.

Guo MS, Wu YY, Liang ZB - Mol. Vis. (2012)

Bottom Line: We investigated the effect of HA on the expression of matrix metalloproteinases (MMP-2 and MMP-9) in cultured trabecular meshwork cells.The expression of MMP-2 and MMP-9 by the two methods significantly increased with HA concentration in a dose-response manner.The lack of HA in aqueous humor can result in a reduction in activities of MMPs and therefore may be involved in the pathogenesis of POAG.

View Article: PubMed Central - PubMed

Affiliation: Second Affiliated Hospital, Fujian Medical University, Quanzhou, Fujian, China.

ABSTRACT

Purpose: A depletion of hyaluronic acid (HA) in patients' eyes may be associated with primary open-angle glaucoma (POAG), but the exact mechanism remains unclear. We investigated the effect of HA on the expression of matrix metalloproteinases (MMP-2 and MMP-9) in cultured trabecular meshwork cells.

Methods: Trabecular meshwork cells were cultured from trabecular tissues obtained from the POAG patients aged 23 to 45. The mRNA expression of MMP-2 and MMP-9 was determined by reverse transcription-polymerase chain reaction, and the protein expression of MMP-2 and MMP-9 by gelatin zymography analysis and qualified by the gel electrophoresis image analysis in different HA concentrations.

Results: The expression of MMP-2 and MMP-9 by the two methods significantly increased with HA concentration in a dose-response manner. Mean values of the MMP-2 expression by the gelatin zymography analysis were 176, 264, 353, and 448 mg/ml, and mean values of the MMP-9 expression were 547, 659, 895, and 1,147 mg/ml, for HA concentration level of 0, 1, 3, and 6 mg/ml, respectively.

Conclusions: In POAG trabecular meshwork cells, the level of HA concentration increases the activities of MMP-2 and MMP-9. The lack of HA in aqueous humor can result in a reduction in activities of MMPs and therefore may be involved in the pathogenesis of POAG.

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Related in: MedlinePlus

Gelatin zymography analysis showing a dose–response relationship of HA on the protein expression of MMP-9. MMP=Matrix metalloproteinases; HA=hyaluronic acid.
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f6: Gelatin zymography analysis showing a dose–response relationship of HA on the protein expression of MMP-9. MMP=Matrix metalloproteinases; HA=hyaluronic acid.

Mentions: Table 2 presents the mRNA expression of MMP-2 and MMP-9 by RT–PCR (Figure 2 and Figure 3), and the protein expression of them by gelatin zymography analysis (Figure 4 and Figure 5) for the three POAG patients. All expressions increased with increasing HA concentration, indicating dose–response relationships between HA and the expressions of MMP-2 and MMP-9 in this range of HA concentration levels. These relationships appear to be linear. An approximately linear increase of the expression of MMP-9 by gelatin zymography analysis was observed in the HA concentration levels of 0 to 6 mg/ml (Figure 6). Therefore, HA was treated as a continuous variable in the regression analyses. According to the regression analyses, all the relationships are statistically significant at a level of p<0.001 (Table 3). Table 3 also presents the effect that sizes of HA had on the expression of MMP-2 and MMP-9 by RT–PCR and gelatin zymography analysis. There was a regression coefficient (effect size) of 100 for HA on the expression of MMP-9 by gelatin zymography analysis. This suggests that MMP-9 expression increased by 100 as per unit (mg/ml) increases in HA concentration based on the gel electrophoresis image analysis camera.


Hyaluronic acid increases MMP-2 and MMP-9 expressions in cultured trabecular meshwork cells from patients with primary open-angle glaucoma.

Guo MS, Wu YY, Liang ZB - Mol. Vis. (2012)

Gelatin zymography analysis showing a dose–response relationship of HA on the protein expression of MMP-9. MMP=Matrix metalloproteinases; HA=hyaluronic acid.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3351412&req=5

f6: Gelatin zymography analysis showing a dose–response relationship of HA on the protein expression of MMP-9. MMP=Matrix metalloproteinases; HA=hyaluronic acid.
Mentions: Table 2 presents the mRNA expression of MMP-2 and MMP-9 by RT–PCR (Figure 2 and Figure 3), and the protein expression of them by gelatin zymography analysis (Figure 4 and Figure 5) for the three POAG patients. All expressions increased with increasing HA concentration, indicating dose–response relationships between HA and the expressions of MMP-2 and MMP-9 in this range of HA concentration levels. These relationships appear to be linear. An approximately linear increase of the expression of MMP-9 by gelatin zymography analysis was observed in the HA concentration levels of 0 to 6 mg/ml (Figure 6). Therefore, HA was treated as a continuous variable in the regression analyses. According to the regression analyses, all the relationships are statistically significant at a level of p<0.001 (Table 3). Table 3 also presents the effect that sizes of HA had on the expression of MMP-2 and MMP-9 by RT–PCR and gelatin zymography analysis. There was a regression coefficient (effect size) of 100 for HA on the expression of MMP-9 by gelatin zymography analysis. This suggests that MMP-9 expression increased by 100 as per unit (mg/ml) increases in HA concentration based on the gel electrophoresis image analysis camera.

Bottom Line: We investigated the effect of HA on the expression of matrix metalloproteinases (MMP-2 and MMP-9) in cultured trabecular meshwork cells.The expression of MMP-2 and MMP-9 by the two methods significantly increased with HA concentration in a dose-response manner.The lack of HA in aqueous humor can result in a reduction in activities of MMPs and therefore may be involved in the pathogenesis of POAG.

View Article: PubMed Central - PubMed

Affiliation: Second Affiliated Hospital, Fujian Medical University, Quanzhou, Fujian, China.

ABSTRACT

Purpose: A depletion of hyaluronic acid (HA) in patients' eyes may be associated with primary open-angle glaucoma (POAG), but the exact mechanism remains unclear. We investigated the effect of HA on the expression of matrix metalloproteinases (MMP-2 and MMP-9) in cultured trabecular meshwork cells.

Methods: Trabecular meshwork cells were cultured from trabecular tissues obtained from the POAG patients aged 23 to 45. The mRNA expression of MMP-2 and MMP-9 was determined by reverse transcription-polymerase chain reaction, and the protein expression of MMP-2 and MMP-9 by gelatin zymography analysis and qualified by the gel electrophoresis image analysis in different HA concentrations.

Results: The expression of MMP-2 and MMP-9 by the two methods significantly increased with HA concentration in a dose-response manner. Mean values of the MMP-2 expression by the gelatin zymography analysis were 176, 264, 353, and 448 mg/ml, and mean values of the MMP-9 expression were 547, 659, 895, and 1,147 mg/ml, for HA concentration level of 0, 1, 3, and 6 mg/ml, respectively.

Conclusions: In POAG trabecular meshwork cells, the level of HA concentration increases the activities of MMP-2 and MMP-9. The lack of HA in aqueous humor can result in a reduction in activities of MMPs and therefore may be involved in the pathogenesis of POAG.

Show MeSH
Related in: MedlinePlus