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Hyaluronic acid increases MMP-2 and MMP-9 expressions in cultured trabecular meshwork cells from patients with primary open-angle glaucoma.

Guo MS, Wu YY, Liang ZB - Mol. Vis. (2012)

Bottom Line: We investigated the effect of HA on the expression of matrix metalloproteinases (MMP-2 and MMP-9) in cultured trabecular meshwork cells.The expression of MMP-2 and MMP-9 by the two methods significantly increased with HA concentration in a dose-response manner.The lack of HA in aqueous humor can result in a reduction in activities of MMPs and therefore may be involved in the pathogenesis of POAG.

View Article: PubMed Central - PubMed

Affiliation: Second Affiliated Hospital, Fujian Medical University, Quanzhou, Fujian, China.

ABSTRACT

Purpose: A depletion of hyaluronic acid (HA) in patients' eyes may be associated with primary open-angle glaucoma (POAG), but the exact mechanism remains unclear. We investigated the effect of HA on the expression of matrix metalloproteinases (MMP-2 and MMP-9) in cultured trabecular meshwork cells.

Methods: Trabecular meshwork cells were cultured from trabecular tissues obtained from the POAG patients aged 23 to 45. The mRNA expression of MMP-2 and MMP-9 was determined by reverse transcription-polymerase chain reaction, and the protein expression of MMP-2 and MMP-9 by gelatin zymography analysis and qualified by the gel electrophoresis image analysis in different HA concentrations.

Results: The expression of MMP-2 and MMP-9 by the two methods significantly increased with HA concentration in a dose-response manner. Mean values of the MMP-2 expression by the gelatin zymography analysis were 176, 264, 353, and 448 mg/ml, and mean values of the MMP-9 expression were 547, 659, 895, and 1,147 mg/ml, for HA concentration level of 0, 1, 3, and 6 mg/ml, respectively.

Conclusions: In POAG trabecular meshwork cells, the level of HA concentration increases the activities of MMP-2 and MMP-9. The lack of HA in aqueous humor can result in a reduction in activities of MMPs and therefore may be involved in the pathogenesis of POAG.

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FN staining, LM staining, NSE staining, and TEM image of trabecular meshwork cells. A: Fibronectin (FN) staining (400×). B: Laminin (LM) staining (400×). C: Neuron-specific enolase (NSE) staining (5,000×). D: Transmission electron microscopy (TEM) image.
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f1: FN staining, LM staining, NSE staining, and TEM image of trabecular meshwork cells. A: Fibronectin (FN) staining (400×). B: Laminin (LM) staining (400×). C: Neuron-specific enolase (NSE) staining (5,000×). D: Transmission electron microscopy (TEM) image.

Mentions: The trabecular cells were successfully cultured from trabecular tissues of the POAG patients using the tissue block method. Immunohistochemical staining for Fibronectin (FN; Figure 1A), laminin (LM) staining (Figure 1B), or neuron-specific enolase (NSE) staining (Figure 1C) were all positive. Transmission electron microscopy indicated that the cells were oval or round shape with plenty of surface microvilli. The major conjunctions of the cells are gap and tight connections. The cytoplasmic organelles were abundant as well as lysosomes, endoplasmic reticulum, mitochondria, phagocytic vesicles, and ribosomes (Figure 1D).


Hyaluronic acid increases MMP-2 and MMP-9 expressions in cultured trabecular meshwork cells from patients with primary open-angle glaucoma.

Guo MS, Wu YY, Liang ZB - Mol. Vis. (2012)

FN staining, LM staining, NSE staining, and TEM image of trabecular meshwork cells. A: Fibronectin (FN) staining (400×). B: Laminin (LM) staining (400×). C: Neuron-specific enolase (NSE) staining (5,000×). D: Transmission electron microscopy (TEM) image.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3351412&req=5

f1: FN staining, LM staining, NSE staining, and TEM image of trabecular meshwork cells. A: Fibronectin (FN) staining (400×). B: Laminin (LM) staining (400×). C: Neuron-specific enolase (NSE) staining (5,000×). D: Transmission electron microscopy (TEM) image.
Mentions: The trabecular cells were successfully cultured from trabecular tissues of the POAG patients using the tissue block method. Immunohistochemical staining for Fibronectin (FN; Figure 1A), laminin (LM) staining (Figure 1B), or neuron-specific enolase (NSE) staining (Figure 1C) were all positive. Transmission electron microscopy indicated that the cells were oval or round shape with plenty of surface microvilli. The major conjunctions of the cells are gap and tight connections. The cytoplasmic organelles were abundant as well as lysosomes, endoplasmic reticulum, mitochondria, phagocytic vesicles, and ribosomes (Figure 1D).

Bottom Line: We investigated the effect of HA on the expression of matrix metalloproteinases (MMP-2 and MMP-9) in cultured trabecular meshwork cells.The expression of MMP-2 and MMP-9 by the two methods significantly increased with HA concentration in a dose-response manner.The lack of HA in aqueous humor can result in a reduction in activities of MMPs and therefore may be involved in the pathogenesis of POAG.

View Article: PubMed Central - PubMed

Affiliation: Second Affiliated Hospital, Fujian Medical University, Quanzhou, Fujian, China.

ABSTRACT

Purpose: A depletion of hyaluronic acid (HA) in patients' eyes may be associated with primary open-angle glaucoma (POAG), but the exact mechanism remains unclear. We investigated the effect of HA on the expression of matrix metalloproteinases (MMP-2 and MMP-9) in cultured trabecular meshwork cells.

Methods: Trabecular meshwork cells were cultured from trabecular tissues obtained from the POAG patients aged 23 to 45. The mRNA expression of MMP-2 and MMP-9 was determined by reverse transcription-polymerase chain reaction, and the protein expression of MMP-2 and MMP-9 by gelatin zymography analysis and qualified by the gel electrophoresis image analysis in different HA concentrations.

Results: The expression of MMP-2 and MMP-9 by the two methods significantly increased with HA concentration in a dose-response manner. Mean values of the MMP-2 expression by the gelatin zymography analysis were 176, 264, 353, and 448 mg/ml, and mean values of the MMP-9 expression were 547, 659, 895, and 1,147 mg/ml, for HA concentration level of 0, 1, 3, and 6 mg/ml, respectively.

Conclusions: In POAG trabecular meshwork cells, the level of HA concentration increases the activities of MMP-2 and MMP-9. The lack of HA in aqueous humor can result in a reduction in activities of MMPs and therefore may be involved in the pathogenesis of POAG.

Show MeSH
Related in: MedlinePlus