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Identification of GABA receptors in chick cornea.

Cheng ZY, Chebib M, Schmid KL - Mol. Vis. (2012)

Bottom Line: The cornea has an important role in vision, is highly innervated and many neurotransmitter receptors are present, e.g., muscarine, melatonin, and dopamine receptors. γ-aminobutyric acid (GABA) is the most important inhibitory neurotransmitter in the retina and central nervous system, but it is unknown whether GABA receptors are present in cornea.The aim of this study was to determine if GABA receptors are located in chick cornea.Corneal tissues were collected from 25, 12-day-old chicks.

View Article: PubMed Central - PubMed

Affiliation: Myopia Center, Department of Ophthalmology, Qilu Hospital, Shandong University, Jinan, Shandong, China.

ABSTRACT

Purpose: The cornea has an important role in vision, is highly innervated and many neurotransmitter receptors are present, e.g., muscarine, melatonin, and dopamine receptors. γ-aminobutyric acid (GABA) is the most important inhibitory neurotransmitter in the retina and central nervous system, but it is unknown whether GABA receptors are present in cornea. The aim of this study was to determine if GABA receptors are located in chick cornea.

Methods: Corneal tissues were collected from 25, 12-day-old chicks. Real time PCR, western blot, and immunohistochemistry were used to determine whether alpha(1) GABA(A), GABA(B), and rho(1) GABA(C) receptors were expressed and located in chick cornea.

Results: Corneal tissue was positive for alpha(1) GABA(A) and rho(1) GABA(C) receptor mRNA (PCR) and protein (western blot) expression but was negative for GABA(B) receptor mRNA and protein. Alpha(1) GABA(A) and rho(1) GABA(C) receptor protein labeling was observed in the corneal epithelium using immunohistochemistry.

Conclusions: These investigations clearly show that chick cornea possesses alpha(1) GABA(A), and rho(1) GABA(C) receptors, but not GABA(B) receptors. The purpose of the alpha(1) GABA(A) and rho(1) GABA(C) receptors in cornea is a fascinating unexplored question.

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Alpha1 GABAA (α1), GABAB (B) and rho1 GABAC (ρ1) receptor protein distribution in chick cornea (C) and retina (R). With antibodies for alpha1 GABAA, GABAB and rho1 GABAC receptors, immunoreactivity was observed in corneal epithelium cell for alpha1 GABAA (α1) and rho1 GABAC (ρ1; yellow arrow), but not for GABAB receptor (B) in the cornea (C). In the retina (R), immunoreactivity was found in the inner plexiform layer, outer plexiform layer, inner nuclear layer, and ganglion cell layer for alpha1 GABAA (α1), GABAB (B) and rho1 GABAC (ρ1) receptors, corresponding to previous reports [6-11,15]. There was essentially no immunoreactivity observed for the negative controls (N.C). The scale bar is 10 µm. Photographs were taken at 40× magnification.
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f3: Alpha1 GABAA (α1), GABAB (B) and rho1 GABAC (ρ1) receptor protein distribution in chick cornea (C) and retina (R). With antibodies for alpha1 GABAA, GABAB and rho1 GABAC receptors, immunoreactivity was observed in corneal epithelium cell for alpha1 GABAA (α1) and rho1 GABAC (ρ1; yellow arrow), but not for GABAB receptor (B) in the cornea (C). In the retina (R), immunoreactivity was found in the inner plexiform layer, outer plexiform layer, inner nuclear layer, and ganglion cell layer for alpha1 GABAA (α1), GABAB (B) and rho1 GABAC (ρ1) receptors, corresponding to previous reports [6-11,15]. There was essentially no immunoreactivity observed for the negative controls (N.C). The scale bar is 10 µm. Photographs were taken at 40× magnification.

Mentions: Alpha1 GABAA, and rho1 GABAC immunoreactivity was observed in chick corneal epithelium, was not observed in the stroma and not in the endothelium. No corneal layer displayed immunoreactivity to GABAB antibodies. In the retina, immunoreactivity for each of the antibodies was observed in the inner plexiform layer, outer plexiform layer, inner nuclear layer and ganglion cell layer, corresponding with previous published data [6-11,15]. There was essentially no immunoreactivity observed in the negative controls tissues (Figure 3).


Identification of GABA receptors in chick cornea.

Cheng ZY, Chebib M, Schmid KL - Mol. Vis. (2012)

Alpha1 GABAA (α1), GABAB (B) and rho1 GABAC (ρ1) receptor protein distribution in chick cornea (C) and retina (R). With antibodies for alpha1 GABAA, GABAB and rho1 GABAC receptors, immunoreactivity was observed in corneal epithelium cell for alpha1 GABAA (α1) and rho1 GABAC (ρ1; yellow arrow), but not for GABAB receptor (B) in the cornea (C). In the retina (R), immunoreactivity was found in the inner plexiform layer, outer plexiform layer, inner nuclear layer, and ganglion cell layer for alpha1 GABAA (α1), GABAB (B) and rho1 GABAC (ρ1) receptors, corresponding to previous reports [6-11,15]. There was essentially no immunoreactivity observed for the negative controls (N.C). The scale bar is 10 µm. Photographs were taken at 40× magnification.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3351410&req=5

f3: Alpha1 GABAA (α1), GABAB (B) and rho1 GABAC (ρ1) receptor protein distribution in chick cornea (C) and retina (R). With antibodies for alpha1 GABAA, GABAB and rho1 GABAC receptors, immunoreactivity was observed in corneal epithelium cell for alpha1 GABAA (α1) and rho1 GABAC (ρ1; yellow arrow), but not for GABAB receptor (B) in the cornea (C). In the retina (R), immunoreactivity was found in the inner plexiform layer, outer plexiform layer, inner nuclear layer, and ganglion cell layer for alpha1 GABAA (α1), GABAB (B) and rho1 GABAC (ρ1) receptors, corresponding to previous reports [6-11,15]. There was essentially no immunoreactivity observed for the negative controls (N.C). The scale bar is 10 µm. Photographs were taken at 40× magnification.
Mentions: Alpha1 GABAA, and rho1 GABAC immunoreactivity was observed in chick corneal epithelium, was not observed in the stroma and not in the endothelium. No corneal layer displayed immunoreactivity to GABAB antibodies. In the retina, immunoreactivity for each of the antibodies was observed in the inner plexiform layer, outer plexiform layer, inner nuclear layer and ganglion cell layer, corresponding with previous published data [6-11,15]. There was essentially no immunoreactivity observed in the negative controls tissues (Figure 3).

Bottom Line: The cornea has an important role in vision, is highly innervated and many neurotransmitter receptors are present, e.g., muscarine, melatonin, and dopamine receptors. γ-aminobutyric acid (GABA) is the most important inhibitory neurotransmitter in the retina and central nervous system, but it is unknown whether GABA receptors are present in cornea.The aim of this study was to determine if GABA receptors are located in chick cornea.Corneal tissues were collected from 25, 12-day-old chicks.

View Article: PubMed Central - PubMed

Affiliation: Myopia Center, Department of Ophthalmology, Qilu Hospital, Shandong University, Jinan, Shandong, China.

ABSTRACT

Purpose: The cornea has an important role in vision, is highly innervated and many neurotransmitter receptors are present, e.g., muscarine, melatonin, and dopamine receptors. γ-aminobutyric acid (GABA) is the most important inhibitory neurotransmitter in the retina and central nervous system, but it is unknown whether GABA receptors are present in cornea. The aim of this study was to determine if GABA receptors are located in chick cornea.

Methods: Corneal tissues were collected from 25, 12-day-old chicks. Real time PCR, western blot, and immunohistochemistry were used to determine whether alpha(1) GABA(A), GABA(B), and rho(1) GABA(C) receptors were expressed and located in chick cornea.

Results: Corneal tissue was positive for alpha(1) GABA(A) and rho(1) GABA(C) receptor mRNA (PCR) and protein (western blot) expression but was negative for GABA(B) receptor mRNA and protein. Alpha(1) GABA(A) and rho(1) GABA(C) receptor protein labeling was observed in the corneal epithelium using immunohistochemistry.

Conclusions: These investigations clearly show that chick cornea possesses alpha(1) GABA(A), and rho(1) GABA(C) receptors, but not GABA(B) receptors. The purpose of the alpha(1) GABA(A) and rho(1) GABA(C) receptors in cornea is a fascinating unexplored question.

Show MeSH
Related in: MedlinePlus