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Identification of GABA receptors in chick cornea.

Cheng ZY, Chebib M, Schmid KL - Mol. Vis. (2012)

Bottom Line: The cornea has an important role in vision, is highly innervated and many neurotransmitter receptors are present, e.g., muscarine, melatonin, and dopamine receptors. γ-aminobutyric acid (GABA) is the most important inhibitory neurotransmitter in the retina and central nervous system, but it is unknown whether GABA receptors are present in cornea.The aim of this study was to determine if GABA receptors are located in chick cornea.Corneal tissues were collected from 25, 12-day-old chicks.

View Article: PubMed Central - PubMed

Affiliation: Myopia Center, Department of Ophthalmology, Qilu Hospital, Shandong University, Jinan, Shandong, China.

ABSTRACT

Purpose: The cornea has an important role in vision, is highly innervated and many neurotransmitter receptors are present, e.g., muscarine, melatonin, and dopamine receptors. γ-aminobutyric acid (GABA) is the most important inhibitory neurotransmitter in the retina and central nervous system, but it is unknown whether GABA receptors are present in cornea. The aim of this study was to determine if GABA receptors are located in chick cornea.

Methods: Corneal tissues were collected from 25, 12-day-old chicks. Real time PCR, western blot, and immunohistochemistry were used to determine whether alpha(1) GABA(A), GABA(B), and rho(1) GABA(C) receptors were expressed and located in chick cornea.

Results: Corneal tissue was positive for alpha(1) GABA(A) and rho(1) GABA(C) receptor mRNA (PCR) and protein (western blot) expression but was negative for GABA(B) receptor mRNA and protein. Alpha(1) GABA(A) and rho(1) GABA(C) receptor protein labeling was observed in the corneal epithelium using immunohistochemistry.

Conclusions: These investigations clearly show that chick cornea possesses alpha(1) GABA(A), and rho(1) GABA(C) receptors, but not GABA(B) receptors. The purpose of the alpha(1) GABA(A) and rho(1) GABA(C) receptors in cornea is a fascinating unexplored question.

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Related in: MedlinePlus

Western blot analysis of alpha1 GABAA, GABAB and rho1 GABAC receptors in the chick cornea and retina. A: This panel shows one intense band (approximately 43 kDa) for β-actin and one intense band (approximately 51 kDa) for alpha1 GABAA receptors (α1) both in cornea (C) and in retina (R). B: This panel shows one intense band (approximately 43 kDa) for β-actin both in cornea (C) and in retina (R), and one intense band (approximately 130 kDa) for GABAB receptors (B) in retina (R) but not in cornea (C). C: This panel shows one intense band (approximately 43 kDa) for β-actin, and one intense band (approximately 48 kDa) for rho1 GABAC receptors (ρ1) both in cornea (C) and in retina (R).
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f2: Western blot analysis of alpha1 GABAA, GABAB and rho1 GABAC receptors in the chick cornea and retina. A: This panel shows one intense band (approximately 43 kDa) for β-actin and one intense band (approximately 51 kDa) for alpha1 GABAA receptors (α1) both in cornea (C) and in retina (R). B: This panel shows one intense band (approximately 43 kDa) for β-actin both in cornea (C) and in retina (R), and one intense band (approximately 130 kDa) for GABAB receptors (B) in retina (R) but not in cornea (C). C: This panel shows one intense band (approximately 43 kDa) for β-actin, and one intense band (approximately 48 kDa) for rho1 GABAC receptors (ρ1) both in cornea (C) and in retina (R).

Mentions: Using antibodies for alpha1 GABAA receptors, one intense band (approximately 51 kDa) was detected in all chick corneal and retinal samples. Using antibodies for GABAB receptors, one intense band (approximately 130 kDa) was detected in the chick retina samples but was not present in the corneal samples. Using antibodies for rho1 GABAC receptors, one intense band (approximately 48 kDa) was detected in the chick corneal and retinal samples. Using antibodies for β-actin, one intense band (approximately 43 kDa) was detected in all the chick corneal and retinal samples (Figure 2).


Identification of GABA receptors in chick cornea.

Cheng ZY, Chebib M, Schmid KL - Mol. Vis. (2012)

Western blot analysis of alpha1 GABAA, GABAB and rho1 GABAC receptors in the chick cornea and retina. A: This panel shows one intense band (approximately 43 kDa) for β-actin and one intense band (approximately 51 kDa) for alpha1 GABAA receptors (α1) both in cornea (C) and in retina (R). B: This panel shows one intense band (approximately 43 kDa) for β-actin both in cornea (C) and in retina (R), and one intense band (approximately 130 kDa) for GABAB receptors (B) in retina (R) but not in cornea (C). C: This panel shows one intense band (approximately 43 kDa) for β-actin, and one intense band (approximately 48 kDa) for rho1 GABAC receptors (ρ1) both in cornea (C) and in retina (R).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3351410&req=5

f2: Western blot analysis of alpha1 GABAA, GABAB and rho1 GABAC receptors in the chick cornea and retina. A: This panel shows one intense band (approximately 43 kDa) for β-actin and one intense band (approximately 51 kDa) for alpha1 GABAA receptors (α1) both in cornea (C) and in retina (R). B: This panel shows one intense band (approximately 43 kDa) for β-actin both in cornea (C) and in retina (R), and one intense band (approximately 130 kDa) for GABAB receptors (B) in retina (R) but not in cornea (C). C: This panel shows one intense band (approximately 43 kDa) for β-actin, and one intense band (approximately 48 kDa) for rho1 GABAC receptors (ρ1) both in cornea (C) and in retina (R).
Mentions: Using antibodies for alpha1 GABAA receptors, one intense band (approximately 51 kDa) was detected in all chick corneal and retinal samples. Using antibodies for GABAB receptors, one intense band (approximately 130 kDa) was detected in the chick retina samples but was not present in the corneal samples. Using antibodies for rho1 GABAC receptors, one intense band (approximately 48 kDa) was detected in the chick corneal and retinal samples. Using antibodies for β-actin, one intense band (approximately 43 kDa) was detected in all the chick corneal and retinal samples (Figure 2).

Bottom Line: The cornea has an important role in vision, is highly innervated and many neurotransmitter receptors are present, e.g., muscarine, melatonin, and dopamine receptors. γ-aminobutyric acid (GABA) is the most important inhibitory neurotransmitter in the retina and central nervous system, but it is unknown whether GABA receptors are present in cornea.The aim of this study was to determine if GABA receptors are located in chick cornea.Corneal tissues were collected from 25, 12-day-old chicks.

View Article: PubMed Central - PubMed

Affiliation: Myopia Center, Department of Ophthalmology, Qilu Hospital, Shandong University, Jinan, Shandong, China.

ABSTRACT

Purpose: The cornea has an important role in vision, is highly innervated and many neurotransmitter receptors are present, e.g., muscarine, melatonin, and dopamine receptors. γ-aminobutyric acid (GABA) is the most important inhibitory neurotransmitter in the retina and central nervous system, but it is unknown whether GABA receptors are present in cornea. The aim of this study was to determine if GABA receptors are located in chick cornea.

Methods: Corneal tissues were collected from 25, 12-day-old chicks. Real time PCR, western blot, and immunohistochemistry were used to determine whether alpha(1) GABA(A), GABA(B), and rho(1) GABA(C) receptors were expressed and located in chick cornea.

Results: Corneal tissue was positive for alpha(1) GABA(A) and rho(1) GABA(C) receptor mRNA (PCR) and protein (western blot) expression but was negative for GABA(B) receptor mRNA and protein. Alpha(1) GABA(A) and rho(1) GABA(C) receptor protein labeling was observed in the corneal epithelium using immunohistochemistry.

Conclusions: These investigations clearly show that chick cornea possesses alpha(1) GABA(A), and rho(1) GABA(C) receptors, but not GABA(B) receptors. The purpose of the alpha(1) GABA(A) and rho(1) GABA(C) receptors in cornea is a fascinating unexplored question.

Show MeSH
Related in: MedlinePlus