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Corneal crosslinking with genipin, comparison with UV-riboflavin in ex-vivo model.

Avila MY, Gerena VA, Navia JL - Mol. Vis. (2012)

Bottom Line: To investigate the efficacy and safety of Genipin and UV-riboflavin crosslinking (UV-CLX) in corneal crosslinking.Corneal crosslinking was similar between UV-CLX and genipin with minimal toxicity to endothelial cells.Stiffened corneas by any method induced substancially higher IOP elevation when ocular volume is increased.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Facultad de Medicina, Universidad Nacional de Colombia, Bogota DC, Colombia. myavilac@unal.edu.co

ABSTRACT

Purpose: To investigate the efficacy and safety of Genipin and UV-riboflavin crosslinking (UV-CLX) in corneal crosslinking.

Methods: Porcine eyes were separated in groups for each crosslinker, genipin 0.25% UV-CLX (clinical crosslinking procedure), glutaraldehyde 0.1% (gold standard crosslinker), and control eyes. Intraocular pressure (IOP) was continuously monitored by a pressure sensor cannulated to the anterior chamber and the volume was changed. The changes in ocular pressure as a function of change of the ocular volume were evaluated. Ocular rigidity was calculated as the exponential of polynomial quadratic fit. Endothelial damage was evaluated in a viability assay with alizarin red staining as the changes in cell counts.

Results: Significant changes in IOP were observed in the globes were the cornea was stiffened with genipin and UV-CLX treatment (volume 200 μl: Genipin 19.4 mmHg, UVCRX 18.8 mmHg, glutaraldehide 23.9 mmHg, versus control 14.7 mmHg, and 400 μl genipin 31.5 mmHg, UV-CLX 26.0 mmHg, glutaraldehide 37.3 mmHg versus control 18.7 mmHg). The mean ocular ridigity coefficient was genipin 0.0078 mmHg/μl, UV-CLX 0.0065 mmHg/μl, glutaraldehide 0.0092 mmHg/μl, and 0.0046 mmHg/μl for control eyes. Endothelial cell damage was 5.9±1.8% (control), 10.3±1.7% (UV-CLX), 9.4±1.5% (Genipin 0.25%), and 40.1±6.2% (glutaraldehide). Some granules were observed in the UV-CLX group. Reduction of keratocites was observed in the UV CRX crosslinking.

Conclusions: Corneal crosslinking was similar between UV-CLX and genipin with minimal toxicity to endothelial cells. Stiffened corneas by any method induced substancially higher IOP elevation when ocular volume is increased.

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Keratocyte change with different crosslinkers.
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f6: Keratocyte change with different crosslinkers.

Mentions: A reduction of keratocytes was observed in the UVCRX group (40±5 cells by field) in comparison with Control (70±10 cells by field), genipin (72±8 cells by field) and glutaraldehyde (64±9 cells by field; Figure 6).


Corneal crosslinking with genipin, comparison with UV-riboflavin in ex-vivo model.

Avila MY, Gerena VA, Navia JL - Mol. Vis. (2012)

Keratocyte change with different crosslinkers.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3351405&req=5

f6: Keratocyte change with different crosslinkers.
Mentions: A reduction of keratocytes was observed in the UVCRX group (40±5 cells by field) in comparison with Control (70±10 cells by field), genipin (72±8 cells by field) and glutaraldehyde (64±9 cells by field; Figure 6).

Bottom Line: To investigate the efficacy and safety of Genipin and UV-riboflavin crosslinking (UV-CLX) in corneal crosslinking.Corneal crosslinking was similar between UV-CLX and genipin with minimal toxicity to endothelial cells.Stiffened corneas by any method induced substancially higher IOP elevation when ocular volume is increased.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Facultad de Medicina, Universidad Nacional de Colombia, Bogota DC, Colombia. myavilac@unal.edu.co

ABSTRACT

Purpose: To investigate the efficacy and safety of Genipin and UV-riboflavin crosslinking (UV-CLX) in corneal crosslinking.

Methods: Porcine eyes were separated in groups for each crosslinker, genipin 0.25% UV-CLX (clinical crosslinking procedure), glutaraldehyde 0.1% (gold standard crosslinker), and control eyes. Intraocular pressure (IOP) was continuously monitored by a pressure sensor cannulated to the anterior chamber and the volume was changed. The changes in ocular pressure as a function of change of the ocular volume were evaluated. Ocular rigidity was calculated as the exponential of polynomial quadratic fit. Endothelial damage was evaluated in a viability assay with alizarin red staining as the changes in cell counts.

Results: Significant changes in IOP were observed in the globes were the cornea was stiffened with genipin and UV-CLX treatment (volume 200 μl: Genipin 19.4 mmHg, UVCRX 18.8 mmHg, glutaraldehide 23.9 mmHg, versus control 14.7 mmHg, and 400 μl genipin 31.5 mmHg, UV-CLX 26.0 mmHg, glutaraldehide 37.3 mmHg versus control 18.7 mmHg). The mean ocular ridigity coefficient was genipin 0.0078 mmHg/μl, UV-CLX 0.0065 mmHg/μl, glutaraldehide 0.0092 mmHg/μl, and 0.0046 mmHg/μl for control eyes. Endothelial cell damage was 5.9±1.8% (control), 10.3±1.7% (UV-CLX), 9.4±1.5% (Genipin 0.25%), and 40.1±6.2% (glutaraldehide). Some granules were observed in the UV-CLX group. Reduction of keratocites was observed in the UV CRX crosslinking.

Conclusions: Corneal crosslinking was similar between UV-CLX and genipin with minimal toxicity to endothelial cells. Stiffened corneas by any method induced substancially higher IOP elevation when ocular volume is increased.

Show MeSH
Related in: MedlinePlus