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Corneal crosslinking with genipin, comparison with UV-riboflavin in ex-vivo model.

Avila MY, Gerena VA, Navia JL - Mol. Vis. (2012)

Bottom Line: To investigate the efficacy and safety of Genipin and UV-riboflavin crosslinking (UV-CLX) in corneal crosslinking.Corneal crosslinking was similar between UV-CLX and genipin with minimal toxicity to endothelial cells.Stiffened corneas by any method induced substancially higher IOP elevation when ocular volume is increased.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Facultad de Medicina, Universidad Nacional de Colombia, Bogota DC, Colombia. myavilac@unal.edu.co

ABSTRACT

Purpose: To investigate the efficacy and safety of Genipin and UV-riboflavin crosslinking (UV-CLX) in corneal crosslinking.

Methods: Porcine eyes were separated in groups for each crosslinker, genipin 0.25% UV-CLX (clinical crosslinking procedure), glutaraldehyde 0.1% (gold standard crosslinker), and control eyes. Intraocular pressure (IOP) was continuously monitored by a pressure sensor cannulated to the anterior chamber and the volume was changed. The changes in ocular pressure as a function of change of the ocular volume were evaluated. Ocular rigidity was calculated as the exponential of polynomial quadratic fit. Endothelial damage was evaluated in a viability assay with alizarin red staining as the changes in cell counts.

Results: Significant changes in IOP were observed in the globes were the cornea was stiffened with genipin and UV-CLX treatment (volume 200 μl: Genipin 19.4 mmHg, UVCRX 18.8 mmHg, glutaraldehide 23.9 mmHg, versus control 14.7 mmHg, and 400 μl genipin 31.5 mmHg, UV-CLX 26.0 mmHg, glutaraldehide 37.3 mmHg versus control 18.7 mmHg). The mean ocular ridigity coefficient was genipin 0.0078 mmHg/μl, UV-CLX 0.0065 mmHg/μl, glutaraldehide 0.0092 mmHg/μl, and 0.0046 mmHg/μl for control eyes. Endothelial cell damage was 5.9±1.8% (control), 10.3±1.7% (UV-CLX), 9.4±1.5% (Genipin 0.25%), and 40.1±6.2% (glutaraldehide). Some granules were observed in the UV-CLX group. Reduction of keratocites was observed in the UV CRX crosslinking.

Conclusions: Corneal crosslinking was similar between UV-CLX and genipin with minimal toxicity to endothelial cells. Stiffened corneas by any method induced substancially higher IOP elevation when ocular volume is increased.

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Related in: MedlinePlus

Representative traces of IOP Changes in treated eyes with different crosslinkers. Every vertical square correspond to 5 mmHg.
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f2: Representative traces of IOP Changes in treated eyes with different crosslinkers. Every vertical square correspond to 5 mmHg.

Mentions: Eyes were canulated in the anterior chamber, one needle was conected to the infussion system with BSS and the other needle was conected to a pressure transductor connected to a digital converter signal, and a trace of the pressure is obtained (Figure 1). Volume was injected with a pressure pump until a pressure of 10 mmHg is achieved and then 200 μl of volume steps were injected every 5 min. The pressure was monitored continuously (Figure 2). Then the relationship between volume and pressure is obtained and the ocular rigidity is calculated as the exponential of quadratic fit.


Corneal crosslinking with genipin, comparison with UV-riboflavin in ex-vivo model.

Avila MY, Gerena VA, Navia JL - Mol. Vis. (2012)

Representative traces of IOP Changes in treated eyes with different crosslinkers. Every vertical square correspond to 5 mmHg.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3351405&req=5

f2: Representative traces of IOP Changes in treated eyes with different crosslinkers. Every vertical square correspond to 5 mmHg.
Mentions: Eyes were canulated in the anterior chamber, one needle was conected to the infussion system with BSS and the other needle was conected to a pressure transductor connected to a digital converter signal, and a trace of the pressure is obtained (Figure 1). Volume was injected with a pressure pump until a pressure of 10 mmHg is achieved and then 200 μl of volume steps were injected every 5 min. The pressure was monitored continuously (Figure 2). Then the relationship between volume and pressure is obtained and the ocular rigidity is calculated as the exponential of quadratic fit.

Bottom Line: To investigate the efficacy and safety of Genipin and UV-riboflavin crosslinking (UV-CLX) in corneal crosslinking.Corneal crosslinking was similar between UV-CLX and genipin with minimal toxicity to endothelial cells.Stiffened corneas by any method induced substancially higher IOP elevation when ocular volume is increased.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Facultad de Medicina, Universidad Nacional de Colombia, Bogota DC, Colombia. myavilac@unal.edu.co

ABSTRACT

Purpose: To investigate the efficacy and safety of Genipin and UV-riboflavin crosslinking (UV-CLX) in corneal crosslinking.

Methods: Porcine eyes were separated in groups for each crosslinker, genipin 0.25% UV-CLX (clinical crosslinking procedure), glutaraldehyde 0.1% (gold standard crosslinker), and control eyes. Intraocular pressure (IOP) was continuously monitored by a pressure sensor cannulated to the anterior chamber and the volume was changed. The changes in ocular pressure as a function of change of the ocular volume were evaluated. Ocular rigidity was calculated as the exponential of polynomial quadratic fit. Endothelial damage was evaluated in a viability assay with alizarin red staining as the changes in cell counts.

Results: Significant changes in IOP were observed in the globes were the cornea was stiffened with genipin and UV-CLX treatment (volume 200 μl: Genipin 19.4 mmHg, UVCRX 18.8 mmHg, glutaraldehide 23.9 mmHg, versus control 14.7 mmHg, and 400 μl genipin 31.5 mmHg, UV-CLX 26.0 mmHg, glutaraldehide 37.3 mmHg versus control 18.7 mmHg). The mean ocular ridigity coefficient was genipin 0.0078 mmHg/μl, UV-CLX 0.0065 mmHg/μl, glutaraldehide 0.0092 mmHg/μl, and 0.0046 mmHg/μl for control eyes. Endothelial cell damage was 5.9±1.8% (control), 10.3±1.7% (UV-CLX), 9.4±1.5% (Genipin 0.25%), and 40.1±6.2% (glutaraldehide). Some granules were observed in the UV-CLX group. Reduction of keratocites was observed in the UV CRX crosslinking.

Conclusions: Corneal crosslinking was similar between UV-CLX and genipin with minimal toxicity to endothelial cells. Stiffened corneas by any method induced substancially higher IOP elevation when ocular volume is increased.

Show MeSH
Related in: MedlinePlus