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A continental-wide perspective: the genepool of nuclear encoded ribosomal DNA and single-copy gene sequences in North American Boechera (Brassicaceae).

Kiefer C, Koch MA - PLoS ONE (2012)

Bottom Line: Together with the network analysis confirming this result this can be interpreted as an indication for multiple, independent, and rapid diversification events.Our results provide first direct evidence for enormous reticulate evolution in the entire genus and give further insights into the evolutionary history of this complex genus on a continental scale.In addition two novel single-copy gene markers, orthologues of the Arabidopsis thaliana genes At2g25920 and At3g18900, were analyzed for subsets of taxa and confirmed the findings obtained through the ITS data.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Developmental Biology, Max Planck Institute for Plant Breeding Research, Cologne, Germany.

ABSTRACT
74 of the currently accepted 111 taxa of the North American genus Boechera (Brassicaceae) were subject to pyhlogenetic reconstruction and network analysis. The dataset comprised 911 accessions for which ITS sequences were analyzed. Phylogenetic analyses yielded largely unresolved trees. Together with the network analysis confirming this result this can be interpreted as an indication for multiple, independent, and rapid diversification events. Network analyses were superimposed with datasets describing i) geographical distribution, ii) taxonomy, iii) reproductive mode, and iv) distribution history based on phylogeographic evidence. Our results provide first direct evidence for enormous reticulate evolution in the entire genus and give further insights into the evolutionary history of this complex genus on a continental scale. In addition two novel single-copy gene markers, orthologues of the Arabidopsis thaliana genes At2g25920 and At3g18900, were analyzed for subsets of taxa and confirmed the findings obtained through the ITS data.

Show MeSH
Geographical distribution of accessions plotted on statistical parsimony network based on 149 ITS types.Accessions were sorted into geographical groups (see colour legend of Figure) and distribution of accessions sharing an ITS type over geographical regions was calculated and is displayed as pie charts. There is a trend for ITS type lineages to occur more in particular geographical regions than in others indicating (at least past) gene flow throughout that region.
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pone-0036491-g004: Geographical distribution of accessions plotted on statistical parsimony network based on 149 ITS types.Accessions were sorted into geographical groups (see colour legend of Figure) and distribution of accessions sharing an ITS type over geographical regions was calculated and is displayed as pie charts. There is a trend for ITS type lineages to occur more in particular geographical regions than in others indicating (at least past) gene flow throughout that region.

Mentions: The network reconstruction based on 149 representative ITS types yielded a reticulate network. ITS type H was found to have the highest probability to be at the centre of the network. Other ancestral ITS types were AD, G, F, V, AZ and the previously not included ITS type W. They were all centres for new lineages being derived from them. In order to facilitate interpretation of the network information on cpDNA lineage as defined in [7] and [8], as well as geographic origin, taxon identity and reproductive mode were plotted on the network in three independent Figures (Figures 2, 3, 4, 5).


A continental-wide perspective: the genepool of nuclear encoded ribosomal DNA and single-copy gene sequences in North American Boechera (Brassicaceae).

Kiefer C, Koch MA - PLoS ONE (2012)

Geographical distribution of accessions plotted on statistical parsimony network based on 149 ITS types.Accessions were sorted into geographical groups (see colour legend of Figure) and distribution of accessions sharing an ITS type over geographical regions was calculated and is displayed as pie charts. There is a trend for ITS type lineages to occur more in particular geographical regions than in others indicating (at least past) gene flow throughout that region.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3351400&req=5

pone-0036491-g004: Geographical distribution of accessions plotted on statistical parsimony network based on 149 ITS types.Accessions were sorted into geographical groups (see colour legend of Figure) and distribution of accessions sharing an ITS type over geographical regions was calculated and is displayed as pie charts. There is a trend for ITS type lineages to occur more in particular geographical regions than in others indicating (at least past) gene flow throughout that region.
Mentions: The network reconstruction based on 149 representative ITS types yielded a reticulate network. ITS type H was found to have the highest probability to be at the centre of the network. Other ancestral ITS types were AD, G, F, V, AZ and the previously not included ITS type W. They were all centres for new lineages being derived from them. In order to facilitate interpretation of the network information on cpDNA lineage as defined in [7] and [8], as well as geographic origin, taxon identity and reproductive mode were plotted on the network in three independent Figures (Figures 2, 3, 4, 5).

Bottom Line: Together with the network analysis confirming this result this can be interpreted as an indication for multiple, independent, and rapid diversification events.Our results provide first direct evidence for enormous reticulate evolution in the entire genus and give further insights into the evolutionary history of this complex genus on a continental scale.In addition two novel single-copy gene markers, orthologues of the Arabidopsis thaliana genes At2g25920 and At3g18900, were analyzed for subsets of taxa and confirmed the findings obtained through the ITS data.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Developmental Biology, Max Planck Institute for Plant Breeding Research, Cologne, Germany.

ABSTRACT
74 of the currently accepted 111 taxa of the North American genus Boechera (Brassicaceae) were subject to pyhlogenetic reconstruction and network analysis. The dataset comprised 911 accessions for which ITS sequences were analyzed. Phylogenetic analyses yielded largely unresolved trees. Together with the network analysis confirming this result this can be interpreted as an indication for multiple, independent, and rapid diversification events. Network analyses were superimposed with datasets describing i) geographical distribution, ii) taxonomy, iii) reproductive mode, and iv) distribution history based on phylogeographic evidence. Our results provide first direct evidence for enormous reticulate evolution in the entire genus and give further insights into the evolutionary history of this complex genus on a continental scale. In addition two novel single-copy gene markers, orthologues of the Arabidopsis thaliana genes At2g25920 and At3g18900, were analyzed for subsets of taxa and confirmed the findings obtained through the ITS data.

Show MeSH