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A continental-wide perspective: the genepool of nuclear encoded ribosomal DNA and single-copy gene sequences in North American Boechera (Brassicaceae).

Kiefer C, Koch MA - PLoS ONE (2012)

Bottom Line: Together with the network analysis confirming this result this can be interpreted as an indication for multiple, independent, and rapid diversification events.Our results provide first direct evidence for enormous reticulate evolution in the entire genus and give further insights into the evolutionary history of this complex genus on a continental scale.In addition two novel single-copy gene markers, orthologues of the Arabidopsis thaliana genes At2g25920 and At3g18900, were analyzed for subsets of taxa and confirmed the findings obtained through the ITS data.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Developmental Biology, Max Planck Institute for Plant Breeding Research, Cologne, Germany.

ABSTRACT
74 of the currently accepted 111 taxa of the North American genus Boechera (Brassicaceae) were subject to pyhlogenetic reconstruction and network analysis. The dataset comprised 911 accessions for which ITS sequences were analyzed. Phylogenetic analyses yielded largely unresolved trees. Together with the network analysis confirming this result this can be interpreted as an indication for multiple, independent, and rapid diversification events. Network analyses were superimposed with datasets describing i) geographical distribution, ii) taxonomy, iii) reproductive mode, and iv) distribution history based on phylogeographic evidence. Our results provide first direct evidence for enormous reticulate evolution in the entire genus and give further insights into the evolutionary history of this complex genus on a continental scale. In addition two novel single-copy gene markers, orthologues of the Arabidopsis thaliana genes At2g25920 and At3g18900, were analyzed for subsets of taxa and confirmed the findings obtained through the ITS data.

Show MeSH
cpDNA haplotype plotted on statistical parsimony network based on 149 ITS types.Roman numbers (I, II, III, V) indicate the lineage of the cpDNA types associated with accessions sharing the ITS type in question: cpDNA lineages are numbered according to [7], [8]; S8 =  ancestral suprahaplotype from [8]; ?  =  no cpDNA data available. It can be concluded that cpDNA lineages and ITS lineages are not congruent in most cases. However, some ITS sequence groups are dominated by a particular cpDNA lineage.
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pone-0036491-g002: cpDNA haplotype plotted on statistical parsimony network based on 149 ITS types.Roman numbers (I, II, III, V) indicate the lineage of the cpDNA types associated with accessions sharing the ITS type in question: cpDNA lineages are numbered according to [7], [8]; S8 =  ancestral suprahaplotype from [8]; ?  =  no cpDNA data available. It can be concluded that cpDNA lineages and ITS lineages are not congruent in most cases. However, some ITS sequence groups are dominated by a particular cpDNA lineage.

Mentions: The network reconstruction based on 149 representative ITS types yielded a reticulate network. ITS type H was found to have the highest probability to be at the centre of the network. Other ancestral ITS types were AD, G, F, V, AZ and the previously not included ITS type W. They were all centres for new lineages being derived from them. In order to facilitate interpretation of the network information on cpDNA lineage as defined in [7] and [8], as well as geographic origin, taxon identity and reproductive mode were plotted on the network in three independent Figures (Figures 2, 3, 4, 5).


A continental-wide perspective: the genepool of nuclear encoded ribosomal DNA and single-copy gene sequences in North American Boechera (Brassicaceae).

Kiefer C, Koch MA - PLoS ONE (2012)

cpDNA haplotype plotted on statistical parsimony network based on 149 ITS types.Roman numbers (I, II, III, V) indicate the lineage of the cpDNA types associated with accessions sharing the ITS type in question: cpDNA lineages are numbered according to [7], [8]; S8 =  ancestral suprahaplotype from [8]; ?  =  no cpDNA data available. It can be concluded that cpDNA lineages and ITS lineages are not congruent in most cases. However, some ITS sequence groups are dominated by a particular cpDNA lineage.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3351400&req=5

pone-0036491-g002: cpDNA haplotype plotted on statistical parsimony network based on 149 ITS types.Roman numbers (I, II, III, V) indicate the lineage of the cpDNA types associated with accessions sharing the ITS type in question: cpDNA lineages are numbered according to [7], [8]; S8 =  ancestral suprahaplotype from [8]; ?  =  no cpDNA data available. It can be concluded that cpDNA lineages and ITS lineages are not congruent in most cases. However, some ITS sequence groups are dominated by a particular cpDNA lineage.
Mentions: The network reconstruction based on 149 representative ITS types yielded a reticulate network. ITS type H was found to have the highest probability to be at the centre of the network. Other ancestral ITS types were AD, G, F, V, AZ and the previously not included ITS type W. They were all centres for new lineages being derived from them. In order to facilitate interpretation of the network information on cpDNA lineage as defined in [7] and [8], as well as geographic origin, taxon identity and reproductive mode were plotted on the network in three independent Figures (Figures 2, 3, 4, 5).

Bottom Line: Together with the network analysis confirming this result this can be interpreted as an indication for multiple, independent, and rapid diversification events.Our results provide first direct evidence for enormous reticulate evolution in the entire genus and give further insights into the evolutionary history of this complex genus on a continental scale.In addition two novel single-copy gene markers, orthologues of the Arabidopsis thaliana genes At2g25920 and At3g18900, were analyzed for subsets of taxa and confirmed the findings obtained through the ITS data.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Developmental Biology, Max Planck Institute for Plant Breeding Research, Cologne, Germany.

ABSTRACT
74 of the currently accepted 111 taxa of the North American genus Boechera (Brassicaceae) were subject to pyhlogenetic reconstruction and network analysis. The dataset comprised 911 accessions for which ITS sequences were analyzed. Phylogenetic analyses yielded largely unresolved trees. Together with the network analysis confirming this result this can be interpreted as an indication for multiple, independent, and rapid diversification events. Network analyses were superimposed with datasets describing i) geographical distribution, ii) taxonomy, iii) reproductive mode, and iv) distribution history based on phylogeographic evidence. Our results provide first direct evidence for enormous reticulate evolution in the entire genus and give further insights into the evolutionary history of this complex genus on a continental scale. In addition two novel single-copy gene markers, orthologues of the Arabidopsis thaliana genes At2g25920 and At3g18900, were analyzed for subsets of taxa and confirmed the findings obtained through the ITS data.

Show MeSH