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Ultrasonic reflection coefficient and surface roughness index of OA articular cartilage: relation to pathological assessment.

Niu HJ, Wang Q, Wang YX, Li DY, Fan YB, Chen WF - BMC Musculoskelet Disord (2012)

Bottom Line: The results showed that URI increased with the OA grade.URI of the normal cartilage samples was significantly lower than the one of the OA cartilage samples.The reflection coefficient of the cartilage surface reduced significantly with the development of OA (p < 0.05), while the reflection coefficient of the cartilage-bone interface increased with the increase of grade.

View Article: PubMed Central - HTML - PubMed

Affiliation: Key Laboratory of the Ministry of Education for Biomechanics and Mechanobiology, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China. hjniu@buaa.edu.cn

ABSTRACT

Background: Early diagnosis of osteoarthritis (OA) is essential for preventing further cartilage destruction and decreasing severe complications. The aims of this study are to explore the relationship between OA pathological grades and quantitative acoustic parameters and to provide more objective criteria for ultrasonic microscopic evaluation of the OA cartilage.

Methods: Articular cartilage samples were prepared from rabbit knees and scanned using ultrasound biomicroscopy (UBM). Three quantitative parameters, including the roughness index of the cartilage surface (URI), the reflection coefficients from the cartilage surface (R) and from the cartilage-bone interface (Rbone) were extracted. The osteoarthritis grades of these cartilage samples were qualitatively assessed by histology according to the grading standards of International Osteoarthritis Institute (OARSI). The relationship between these quantitative parameters and the osteoarthritis grades was explored.

Results: The results showed that URI increased with the OA grade. URI of the normal cartilage samples was significantly lower than the one of the OA cartilage samples. There was no significant difference in URI between the grade 1 cartilage samples and the grade 2 cartilage samples. The reflection coefficient of the cartilage surface reduced significantly with the development of OA (p < 0.05), while the reflection coefficient of the cartilage-bone interface increased with the increase of grade.

Conclusion: High frequency ultrasound measurements can reflect the changes in the surface roughness index and the ultrasound reflection coefficients of the cartilage samples with different OA grades. This study may provide useful information for the quantitative ultrasonic diagnosis of early OA.

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The toluidine blue stained histological sections of (a) normal cartilage, (b) the grade 1 cartilage, (c) the grade 2 cartilage, and (d) the grade 3 cartilage. Matrix staining was homogeneously colored by toluidine blue in (a). The reduction in staining was found in (b-d). The long arrows indicate the superficial zone of articular cartilage. The short arrows show the interface between the bone and the cartilage.
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Figure 3: The toluidine blue stained histological sections of (a) normal cartilage, (b) the grade 1 cartilage, (c) the grade 2 cartilage, and (d) the grade 3 cartilage. Matrix staining was homogeneously colored by toluidine blue in (a). The reduction in staining was found in (b-d). The long arrows indicate the superficial zone of articular cartilage. The short arrows show the interface between the bone and the cartilage.

Mentions: Figure 3 shows the pathological sections (toluidine blue staining) of normal cartilage and three cartilage samples with different OA grades. The pathological sections were taken from the same sites where were scanned by UBM. Figure 3a shows a sample of normal cartilage. The cartilage surface is flat and smooth. The full cartilage matrix is homogeneously stained by toluidine blue. The OA cartilage samples with grade1, 2 and 3 are respectively showed in Figure 3b-d. The surface of the grade 1 cartilage sample became uneven with a slight appearance of fibrillation and was slightly stained by toluidine blue. The toluidine blue staining of the surface of the grade 2 cartilage sample completely disappeared, and the cartilage surface was not flat. An obvious cartilage thinning was found in the OA cartilage with grade 3. The toluidine blue staining of the superficial and mid layers disappeared and the deep tissue was involved into the degradation.


Ultrasonic reflection coefficient and surface roughness index of OA articular cartilage: relation to pathological assessment.

Niu HJ, Wang Q, Wang YX, Li DY, Fan YB, Chen WF - BMC Musculoskelet Disord (2012)

The toluidine blue stained histological sections of (a) normal cartilage, (b) the grade 1 cartilage, (c) the grade 2 cartilage, and (d) the grade 3 cartilage. Matrix staining was homogeneously colored by toluidine blue in (a). The reduction in staining was found in (b-d). The long arrows indicate the superficial zone of articular cartilage. The short arrows show the interface between the bone and the cartilage.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3351369&req=5

Figure 3: The toluidine blue stained histological sections of (a) normal cartilage, (b) the grade 1 cartilage, (c) the grade 2 cartilage, and (d) the grade 3 cartilage. Matrix staining was homogeneously colored by toluidine blue in (a). The reduction in staining was found in (b-d). The long arrows indicate the superficial zone of articular cartilage. The short arrows show the interface between the bone and the cartilage.
Mentions: Figure 3 shows the pathological sections (toluidine blue staining) of normal cartilage and three cartilage samples with different OA grades. The pathological sections were taken from the same sites where were scanned by UBM. Figure 3a shows a sample of normal cartilage. The cartilage surface is flat and smooth. The full cartilage matrix is homogeneously stained by toluidine blue. The OA cartilage samples with grade1, 2 and 3 are respectively showed in Figure 3b-d. The surface of the grade 1 cartilage sample became uneven with a slight appearance of fibrillation and was slightly stained by toluidine blue. The toluidine blue staining of the surface of the grade 2 cartilage sample completely disappeared, and the cartilage surface was not flat. An obvious cartilage thinning was found in the OA cartilage with grade 3. The toluidine blue staining of the superficial and mid layers disappeared and the deep tissue was involved into the degradation.

Bottom Line: The results showed that URI increased with the OA grade.URI of the normal cartilage samples was significantly lower than the one of the OA cartilage samples.The reflection coefficient of the cartilage surface reduced significantly with the development of OA (p < 0.05), while the reflection coefficient of the cartilage-bone interface increased with the increase of grade.

View Article: PubMed Central - HTML - PubMed

Affiliation: Key Laboratory of the Ministry of Education for Biomechanics and Mechanobiology, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China. hjniu@buaa.edu.cn

ABSTRACT

Background: Early diagnosis of osteoarthritis (OA) is essential for preventing further cartilage destruction and decreasing severe complications. The aims of this study are to explore the relationship between OA pathological grades and quantitative acoustic parameters and to provide more objective criteria for ultrasonic microscopic evaluation of the OA cartilage.

Methods: Articular cartilage samples were prepared from rabbit knees and scanned using ultrasound biomicroscopy (UBM). Three quantitative parameters, including the roughness index of the cartilage surface (URI), the reflection coefficients from the cartilage surface (R) and from the cartilage-bone interface (Rbone) were extracted. The osteoarthritis grades of these cartilage samples were qualitatively assessed by histology according to the grading standards of International Osteoarthritis Institute (OARSI). The relationship between these quantitative parameters and the osteoarthritis grades was explored.

Results: The results showed that URI increased with the OA grade. URI of the normal cartilage samples was significantly lower than the one of the OA cartilage samples. There was no significant difference in URI between the grade 1 cartilage samples and the grade 2 cartilage samples. The reflection coefficient of the cartilage surface reduced significantly with the development of OA (p < 0.05), while the reflection coefficient of the cartilage-bone interface increased with the increase of grade.

Conclusion: High frequency ultrasound measurements can reflect the changes in the surface roughness index and the ultrasound reflection coefficients of the cartilage samples with different OA grades. This study may provide useful information for the quantitative ultrasonic diagnosis of early OA.

Show MeSH
Related in: MedlinePlus