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Rapid determination of natural steroidal hormones in saliva for the clinical diagnoses.

Oh JA, Shin HS - Chem Cent J (2012)

Bottom Line: Natural steroidal hormones were detected in the concentration ranges of nd to 0.2 μg/L in human saliva.The salivary testosterone values in the patients with prostatic carcinoma were significantly lower than in normal males.The method may useful in numerous clinical and health-related diagnoses.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Environmental Education, Kongju National University, Kongju, Korea 314-701, South Korea. hshin@kongju.ac.kr.

ABSTRACT

Background: Saliva samples are easily collectable and non-invasive, and the monitoring of natural steroidal hormones, such as estrone (E1), 17β-estradiol (E2), estriol (E3), progesterone (P), and testosterone (T), in saliva has attracted much attention due to its numerous potential clinical and health-related applications. Because E1, E2, E3, P and T are useful indicators in numerous clinical and health-related diagnoses, there is a need for simultaneous determination.

Results: A gas chromatography-mass spectrometric assay was developed for rapid simultaneous determination of E1, E2, E3, P and T in saliva for clinical diagnoses. Extraction was achieved with a liquid extraction using 3.0 mL of pentane. The extract was dried and silylated with N-methyl-N-(trimethylsilyl) trifluoroacetamide/NH4I (100:2) under a catalysis of 1.5% dithioerythritol for 10 min at 90°C. The accuracy of the analytes was in the range of 96% to 112% at concentrations of 0.05 and 0.10 μg/L (5.0 and 10.0 μg/L for E3), respectively, with relative standard deviations of less than 11%. The lowest quantification limits were from 0.002 to 0.6 μg/L for 1.0 mL of saliva.

Conclusion: Natural steroidal hormones were detected in the concentration ranges of nd to 0.2 μg/L in human saliva. The salivary testosterone values in the patients with prostatic carcinoma were significantly lower than in normal males. The method may useful in numerous clinical and health-related diagnoses.

No MeSH data available.


Related in: MedlinePlus

GC-MS chromatogram after the extraction and derivatization of blank and real saliva sample. The concentrations of estradiol (m/z 414), progesterone (m/z 458) and testosterone (m/z 432) were 0.008 μg/L, 0.013 μg/L and 0.035 μg/L, respectively.
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Figure 2: GC-MS chromatogram after the extraction and derivatization of blank and real saliva sample. The concentrations of estradiol (m/z 414), progesterone (m/z 458) and testosterone (m/z 432) were 0.008 μg/L, 0.013 μg/L and 0.035 μg/L, respectively.

Mentions: Figure 1 and 2 show chromatograms of the bis-TMS-E1, bis-TMS-E2, tri-TMS-E3, bis-TMS-P, bis-TMS-T and bis-TMS-T-d3 in the standard solution and a real sample. The peaks are symmetrical and no tailing can be seen. There are no extraneous peaks observed in the chromatogram of the sample at the analyte retention times. The retention times of bis-TMS-E1, bis-TMS-E2, bis-TMS-T-d3, bis-TMS-T, tri-TMS-E3 and bis-TMS-P were 11.29, 11.42, 11.44, 11.45, 12.70 and 12.79 min, respectively.


Rapid determination of natural steroidal hormones in saliva for the clinical diagnoses.

Oh JA, Shin HS - Chem Cent J (2012)

GC-MS chromatogram after the extraction and derivatization of blank and real saliva sample. The concentrations of estradiol (m/z 414), progesterone (m/z 458) and testosterone (m/z 432) were 0.008 μg/L, 0.013 μg/L and 0.035 μg/L, respectively.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3351364&req=5

Figure 2: GC-MS chromatogram after the extraction and derivatization of blank and real saliva sample. The concentrations of estradiol (m/z 414), progesterone (m/z 458) and testosterone (m/z 432) were 0.008 μg/L, 0.013 μg/L and 0.035 μg/L, respectively.
Mentions: Figure 1 and 2 show chromatograms of the bis-TMS-E1, bis-TMS-E2, tri-TMS-E3, bis-TMS-P, bis-TMS-T and bis-TMS-T-d3 in the standard solution and a real sample. The peaks are symmetrical and no tailing can be seen. There are no extraneous peaks observed in the chromatogram of the sample at the analyte retention times. The retention times of bis-TMS-E1, bis-TMS-E2, bis-TMS-T-d3, bis-TMS-T, tri-TMS-E3 and bis-TMS-P were 11.29, 11.42, 11.44, 11.45, 12.70 and 12.79 min, respectively.

Bottom Line: Natural steroidal hormones were detected in the concentration ranges of nd to 0.2 μg/L in human saliva.The salivary testosterone values in the patients with prostatic carcinoma were significantly lower than in normal males.The method may useful in numerous clinical and health-related diagnoses.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Environmental Education, Kongju National University, Kongju, Korea 314-701, South Korea. hshin@kongju.ac.kr.

ABSTRACT

Background: Saliva samples are easily collectable and non-invasive, and the monitoring of natural steroidal hormones, such as estrone (E1), 17β-estradiol (E2), estriol (E3), progesterone (P), and testosterone (T), in saliva has attracted much attention due to its numerous potential clinical and health-related applications. Because E1, E2, E3, P and T are useful indicators in numerous clinical and health-related diagnoses, there is a need for simultaneous determination.

Results: A gas chromatography-mass spectrometric assay was developed for rapid simultaneous determination of E1, E2, E3, P and T in saliva for clinical diagnoses. Extraction was achieved with a liquid extraction using 3.0 mL of pentane. The extract was dried and silylated with N-methyl-N-(trimethylsilyl) trifluoroacetamide/NH4I (100:2) under a catalysis of 1.5% dithioerythritol for 10 min at 90°C. The accuracy of the analytes was in the range of 96% to 112% at concentrations of 0.05 and 0.10 μg/L (5.0 and 10.0 μg/L for E3), respectively, with relative standard deviations of less than 11%. The lowest quantification limits were from 0.002 to 0.6 μg/L for 1.0 mL of saliva.

Conclusion: Natural steroidal hormones were detected in the concentration ranges of nd to 0.2 μg/L in human saliva. The salivary testosterone values in the patients with prostatic carcinoma were significantly lower than in normal males. The method may useful in numerous clinical and health-related diagnoses.

No MeSH data available.


Related in: MedlinePlus