Limits...
Chemical Composition and In Vitro Cytotoxic Activity of Essential Oil of Leaves of Malus domestica Growing in Western Himalaya (India).

Walia M, Mann TS, Kumar D, Agnihotri VK, Singh B - Evid Based Complement Alternat Med (2012)

Bottom Line: Seventeen compounds accounting for nearly 95.3% of the oil were characterized with the help of capillary GC, GC-MS, and NMR.Major compounds of the oil were characterized as eucalyptol (43.7%), phytol (11.5%), α-farnesene (9.6%), and pentacosane (7.6%).The highest activity showed by essential oil on C-6 cell lines (98.2%) at concentration of 2000 μg/ml compared to control.

View Article: PubMed Central - PubMed

Affiliation: Natural Plant Products Division, CSIR-Institute of Himalayan Bioresource Technology, P.O. Box No. 6, Palampur 176 061 (HP), India.

ABSTRACT
Light pale-colored volatile oil was obtained from fresh leaves of Malus domestica tree, growing in Dhauladhar range of Himalaya (Himachal Pradesh, India), with characteristic eucalyptol dominant fragrance. The oil was found to be a complex mixture of mono-, sesqui-, di-terpenes, phenolics, and aliphatic hydrocarbons. Seventeen compounds accounting for nearly 95.3% of the oil were characterized with the help of capillary GC, GC-MS, and NMR. Major compounds of the oil were characterized as eucalyptol (43.7%), phytol (11.5%), α-farnesene (9.6%), and pentacosane (7.6%). Cytotoxicity of essential oil of leaves of M. domestica was evaluated by sulforhodamine B (SRB) assays. The essential oil of leaves of M. domestica, tested against three cancer cell lines, namely, C-6 (glioma cells), A549 (human lung carcinoma), CHOK1 (Chinese hamster ovary cells), and THP-1 (human acute monocytic leukemia cell). The highest activity showed by essential oil on C-6 cell lines (98.2%) at concentration of 2000 μg/ml compared to control. It is the first paper in literature to exploit the chemical composition and cytotoxic activity of leaves essential oil of M. domestica.

No MeSH data available.


Related in: MedlinePlus

Microscopic images of cells treated with essential oil of leaves of Malus domestica. (a) C-6 cells without any treatment, (b) C-6 cells after 24 hrs of addition with 1000 μg/mL test compound, (c) C-6 cells after 48 hrs of addition with 1000 μg/mL test compound, (d) A549 cells without any treatment, (e) A549 cells after 24 hrs of addition with 2000 μg/mL test compound, (f) A549 cells after 48 hrs of addition with 2000 μg/mL test compound, (g) CHOK1 cells without any treatment, (h) CHOK1 cells after 24 hrs of addition with 2000 μg/mL test compound, (i) CHOK1 cells after 48 hrs of addition with 2000 μg/mL test compound, (j) THP-1 cells without any treatment, (k) THP-1 cells after 24 hrs of addition with 1000 μg/mL test compound, and (l) THP-1 cells after 48 hrs of addition with 1000 μg/mL test compound.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3351209&req=5

fig1: Microscopic images of cells treated with essential oil of leaves of Malus domestica. (a) C-6 cells without any treatment, (b) C-6 cells after 24 hrs of addition with 1000 μg/mL test compound, (c) C-6 cells after 48 hrs of addition with 1000 μg/mL test compound, (d) A549 cells without any treatment, (e) A549 cells after 24 hrs of addition with 2000 μg/mL test compound, (f) A549 cells after 48 hrs of addition with 2000 μg/mL test compound, (g) CHOK1 cells without any treatment, (h) CHOK1 cells after 24 hrs of addition with 2000 μg/mL test compound, (i) CHOK1 cells after 48 hrs of addition with 2000 μg/mL test compound, (j) THP-1 cells without any treatment, (k) THP-1 cells after 24 hrs of addition with 1000 μg/mL test compound, and (l) THP-1 cells after 48 hrs of addition with 1000 μg/mL test compound.

Mentions: The morphological changes of C-6, A549, CHOK1 and THP-1 cells treated with essential oil of leaves of M. domestica for 24 and 48 hours were observed by using Fluorescent microscope (Nikon Eclipse Ti) at magnification 10X (Figure 1).


Chemical Composition and In Vitro Cytotoxic Activity of Essential Oil of Leaves of Malus domestica Growing in Western Himalaya (India).

Walia M, Mann TS, Kumar D, Agnihotri VK, Singh B - Evid Based Complement Alternat Med (2012)

Microscopic images of cells treated with essential oil of leaves of Malus domestica. (a) C-6 cells without any treatment, (b) C-6 cells after 24 hrs of addition with 1000 μg/mL test compound, (c) C-6 cells after 48 hrs of addition with 1000 μg/mL test compound, (d) A549 cells without any treatment, (e) A549 cells after 24 hrs of addition with 2000 μg/mL test compound, (f) A549 cells after 48 hrs of addition with 2000 μg/mL test compound, (g) CHOK1 cells without any treatment, (h) CHOK1 cells after 24 hrs of addition with 2000 μg/mL test compound, (i) CHOK1 cells after 48 hrs of addition with 2000 μg/mL test compound, (j) THP-1 cells without any treatment, (k) THP-1 cells after 24 hrs of addition with 1000 μg/mL test compound, and (l) THP-1 cells after 48 hrs of addition with 1000 μg/mL test compound.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3351209&req=5

fig1: Microscopic images of cells treated with essential oil of leaves of Malus domestica. (a) C-6 cells without any treatment, (b) C-6 cells after 24 hrs of addition with 1000 μg/mL test compound, (c) C-6 cells after 48 hrs of addition with 1000 μg/mL test compound, (d) A549 cells without any treatment, (e) A549 cells after 24 hrs of addition with 2000 μg/mL test compound, (f) A549 cells after 48 hrs of addition with 2000 μg/mL test compound, (g) CHOK1 cells without any treatment, (h) CHOK1 cells after 24 hrs of addition with 2000 μg/mL test compound, (i) CHOK1 cells after 48 hrs of addition with 2000 μg/mL test compound, (j) THP-1 cells without any treatment, (k) THP-1 cells after 24 hrs of addition with 1000 μg/mL test compound, and (l) THP-1 cells after 48 hrs of addition with 1000 μg/mL test compound.
Mentions: The morphological changes of C-6, A549, CHOK1 and THP-1 cells treated with essential oil of leaves of M. domestica for 24 and 48 hours were observed by using Fluorescent microscope (Nikon Eclipse Ti) at magnification 10X (Figure 1).

Bottom Line: Seventeen compounds accounting for nearly 95.3% of the oil were characterized with the help of capillary GC, GC-MS, and NMR.Major compounds of the oil were characterized as eucalyptol (43.7%), phytol (11.5%), α-farnesene (9.6%), and pentacosane (7.6%).The highest activity showed by essential oil on C-6 cell lines (98.2%) at concentration of 2000 μg/ml compared to control.

View Article: PubMed Central - PubMed

Affiliation: Natural Plant Products Division, CSIR-Institute of Himalayan Bioresource Technology, P.O. Box No. 6, Palampur 176 061 (HP), India.

ABSTRACT
Light pale-colored volatile oil was obtained from fresh leaves of Malus domestica tree, growing in Dhauladhar range of Himalaya (Himachal Pradesh, India), with characteristic eucalyptol dominant fragrance. The oil was found to be a complex mixture of mono-, sesqui-, di-terpenes, phenolics, and aliphatic hydrocarbons. Seventeen compounds accounting for nearly 95.3% of the oil were characterized with the help of capillary GC, GC-MS, and NMR. Major compounds of the oil were characterized as eucalyptol (43.7%), phytol (11.5%), α-farnesene (9.6%), and pentacosane (7.6%). Cytotoxicity of essential oil of leaves of M. domestica was evaluated by sulforhodamine B (SRB) assays. The essential oil of leaves of M. domestica, tested against three cancer cell lines, namely, C-6 (glioma cells), A549 (human lung carcinoma), CHOK1 (Chinese hamster ovary cells), and THP-1 (human acute monocytic leukemia cell). The highest activity showed by essential oil on C-6 cell lines (98.2%) at concentration of 2000 μg/ml compared to control. It is the first paper in literature to exploit the chemical composition and cytotoxic activity of leaves essential oil of M. domestica.

No MeSH data available.


Related in: MedlinePlus