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What sequences on high-field MR best depict temporal resolution of experimental ICH and edema formation in mice?

Li M, Akhavan-Sharif RM, Friedlander RM, Du R, Thiex R - J. Biomed. Biotechnol. (2012)

Bottom Line: Values were compared with morphometric analysis of cryosections at the time of final MR imaging.For hematoma quantitation, the Spearman correlation coefficient (r) between T1 signal change and histology was 0.70 (P < 0.04) compared with r = 0.61 (P < 0.09) for T2*.The extent of perihematomal edema formation on cryosections was well reflected on T2 with r = 0.73 (P < 0.03).

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

ABSTRACT

Background and purpose: Pilot study to examine the use of T1-, T2-, and T2*-weighted images for evaluating hematoma size and extent of edema in mouse brain at high field.

Methods: Following collagenase-induced intracerebral hemorrhage, nine mice were imaged at 4.7 T using T1-, T2-, and T2*-weighted images for hematoma and edema quantitation on days 1, 3, 10, and 21 after surgery. Values were compared with morphometric analysis of cryosections at the time of final MR imaging.

Results: For hematoma quantitation, the Spearman correlation coefficient (r) between T1 signal change and histology was 0.70 (P < 0.04) compared with r = 0.61 (P < 0.09) for T2*. The extent of perihematomal edema formation on cryosections was well reflected on T2 with r = 0.73 (P < 0.03).

Conclusions: Within the limits of our pilot study, MR imaging on 4.7 T appears to approximate the temporal changes in hematoma and edema sizes in murine ICH well, thus laying the groundwork for longitudinal studies on hematoma resorption and edema formation.

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Related in: MedlinePlus

T2-weighted RARE spin-echo images at 24 hours (a) show predominantly low-signal hematoma within the left caudate/putamen, consistent with deoxyhemoglobin and intracellular methemoglobin. At 72 hours (b), there is conversion to predominantly high-signal, consistent with extracellular methemoglobin. In the course of hematoma resorption, the core hyperintensity (c) subsequently transforms to a hypointense gliotic scar at 21 days (d). The cryostat section stained with Luxol Fast Blue and cresyl violet [×10] (e) also well delineates the gliotic scar.
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fig4: T2-weighted RARE spin-echo images at 24 hours (a) show predominantly low-signal hematoma within the left caudate/putamen, consistent with deoxyhemoglobin and intracellular methemoglobin. At 72 hours (b), there is conversion to predominantly high-signal, consistent with extracellular methemoglobin. In the course of hematoma resorption, the core hyperintensity (c) subsequently transforms to a hypointense gliotic scar at 21 days (d). The cryostat section stained with Luxol Fast Blue and cresyl violet [×10] (e) also well delineates the gliotic scar.

Mentions: In mice with serial MR imaging up to 72 hours, 10 and 21 days, the predominantly low-signal hematoma within the left caudate/putamen in T1-, T2-, and T2*-weighted images were consistent with deoxyhemoglobin and intracellular methemoglobin after 24 hours. The signal converted to predominantly high intensity at 72 hours to 10 days on T2-weighted RARE spin-echo images, consistent with extracellular methemoglobin (Figure 2). The initially hypointense hematoma on T1-weighted images became hyperintense on days 3 and 10. Even on T2*-weighted images, the signal of the hematoma core became hyperintense at 10 days. On day 21, a low-signal streak was left at the site of the former space-occupying hematoma on T1-, T2-, and T2*-weighted images (Figures 3 and 4).


What sequences on high-field MR best depict temporal resolution of experimental ICH and edema formation in mice?

Li M, Akhavan-Sharif RM, Friedlander RM, Du R, Thiex R - J. Biomed. Biotechnol. (2012)

T2-weighted RARE spin-echo images at 24 hours (a) show predominantly low-signal hematoma within the left caudate/putamen, consistent with deoxyhemoglobin and intracellular methemoglobin. At 72 hours (b), there is conversion to predominantly high-signal, consistent with extracellular methemoglobin. In the course of hematoma resorption, the core hyperintensity (c) subsequently transforms to a hypointense gliotic scar at 21 days (d). The cryostat section stained with Luxol Fast Blue and cresyl violet [×10] (e) also well delineates the gliotic scar.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3351132&req=5

fig4: T2-weighted RARE spin-echo images at 24 hours (a) show predominantly low-signal hematoma within the left caudate/putamen, consistent with deoxyhemoglobin and intracellular methemoglobin. At 72 hours (b), there is conversion to predominantly high-signal, consistent with extracellular methemoglobin. In the course of hematoma resorption, the core hyperintensity (c) subsequently transforms to a hypointense gliotic scar at 21 days (d). The cryostat section stained with Luxol Fast Blue and cresyl violet [×10] (e) also well delineates the gliotic scar.
Mentions: In mice with serial MR imaging up to 72 hours, 10 and 21 days, the predominantly low-signal hematoma within the left caudate/putamen in T1-, T2-, and T2*-weighted images were consistent with deoxyhemoglobin and intracellular methemoglobin after 24 hours. The signal converted to predominantly high intensity at 72 hours to 10 days on T2-weighted RARE spin-echo images, consistent with extracellular methemoglobin (Figure 2). The initially hypointense hematoma on T1-weighted images became hyperintense on days 3 and 10. Even on T2*-weighted images, the signal of the hematoma core became hyperintense at 10 days. On day 21, a low-signal streak was left at the site of the former space-occupying hematoma on T1-, T2-, and T2*-weighted images (Figures 3 and 4).

Bottom Line: Values were compared with morphometric analysis of cryosections at the time of final MR imaging.For hematoma quantitation, the Spearman correlation coefficient (r) between T1 signal change and histology was 0.70 (P < 0.04) compared with r = 0.61 (P < 0.09) for T2*.The extent of perihematomal edema formation on cryosections was well reflected on T2 with r = 0.73 (P < 0.03).

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

ABSTRACT

Background and purpose: Pilot study to examine the use of T1-, T2-, and T2*-weighted images for evaluating hematoma size and extent of edema in mouse brain at high field.

Methods: Following collagenase-induced intracerebral hemorrhage, nine mice were imaged at 4.7 T using T1-, T2-, and T2*-weighted images for hematoma and edema quantitation on days 1, 3, 10, and 21 after surgery. Values were compared with morphometric analysis of cryosections at the time of final MR imaging.

Results: For hematoma quantitation, the Spearman correlation coefficient (r) between T1 signal change and histology was 0.70 (P < 0.04) compared with r = 0.61 (P < 0.09) for T2*. The extent of perihematomal edema formation on cryosections was well reflected on T2 with r = 0.73 (P < 0.03).

Conclusions: Within the limits of our pilot study, MR imaging on 4.7 T appears to approximate the temporal changes in hematoma and edema sizes in murine ICH well, thus laying the groundwork for longitudinal studies on hematoma resorption and edema formation.

Show MeSH
Related in: MedlinePlus